Bir Ekmeklik Buğday Çeşidinde (Triticum aestivum L. cv. Ceyhan 99) Krom Stresine Karşı Fulvik Asitin Etkileri

Bu çalışmada ekmeklik buğdayda krom stresine karşı fulvik asitin (FA) fotosentetik pigment ve malondialdehid (MDA) içeriği üzerine etkileri araştırıldı. Bitki materyali olarak Amasya iline bağlı Suluova ilçesinde yetişen ekmeklik buğday çeşidi Ceyhan 99 kullanıldı. Buğday bitkileri çimlendikten sonra saksılara aktarılarak, laboratuvarda 18/6 saat ışık/karanlık rejimi altında yetiştirildi. Buğday fideleri iki gruba ayrılarak birinci gruba 0.10, 0.20, 0.30, 0.50 mM krom çözeltisi, ikinci gruba ise aynı konsantrasyonlarda krom çözeltisi ve 1.5 mg L-1 FA çözeltisi uygulandı. Yapılan çalışmada krom stresi uygulamasına bağlı olarak klorofil a, klorofil b, toplam klorofil ve karotenoid içeriğinde yalnızca krom uygulanan grupta, FA+krom uygulanan gruba göre azalma tespit edilmiştir. Buna karşın yalnızca krom uygulanan grupta, FA+krom uygulanan gruba göre Klorofil a b-1 oranı ve MDA içeriğinde artış belirlenmiştir. Bu çalışma FA’in buğday bitkilerinde krom stresine karşı önemli bir role sahip olduğunu göstermektedir. Elde edilen verilere göre, buğday bitkilerine FA uygulamasının kromun zararlı etkilerini azaltabileceği görülmüştür.

Anahtar Kelimeler:

Ağır metal, fotosentetik pigment, karotenoid, klorofil, malondialdehid

The Effects of Fulvic Acid Against on Chromium Stress in a Bread Wheat Variety (Triticum aestivum L. cv. Ceyhan 99)

In this study, effects of fulvic acid (FA) on photosynthetic pigment and malondialdehyde (MDA) content against chromium stress were investigated in Ceyhan 99, a bread wheat variety. As a plant material, a kind of bread wheat (Ceyhan 99) which is grown in Suluova, Amasya was used. After the wheat plants were germinated, they were transferred to a pot and grown under the light/dark regime for 18/6 hours at the laboratory. The wheat seedlings was divided into two groups and the first group was treated with 0.10, 0.20, 0.30, 0.50 mM chromium solution, the second group in the same concentrations with chromium solution and 1.5 mg L-1 FA solution. In this study chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were found to be decreased in the chromium-treated group compared to FA + chromium treated group depending on the chromium stress application. However, only Chlorophyll a b-1 ratio and MDA content were increased in chromium treated group compared to FA + chromium treated group. This study shows that FA has an important role against chromium stress in wheat plants. According to the obtained data, it was observed that the application of FA to wheat plants could reduce the harmful effects of chromium.

Keywords:

Heavy metal, photosynthetic pigment, carotenoid, chlorophyll, malondialdehyde,

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