2,6- Kinolindiol Bileşiğinin Antioksidan Kapasitesinin Belirlenmesi

Kinolin grubu bileşikleri, canlı metabolizması, boya ve ilaç sektöründe geniş çapta kullanılmaya başlamıştır. Bu nedenle çeşitli kinolin türevleri sentezlenmekte ve sentez yöntemleri geliştirilmektedir. Endüstriel açıdan kinolin bileşiklerinin sentezi ayrı bir alan oluşturmaktadır. 2,6-kinolindiol bir kinolin türevidir. Bu çalışmada; Fe3+-Fe2+ transformasyonu metoduna göre indirgeme kapasitesi, CUPRAK metoduna göre Cu2+-Cu+ indirgeme kapasitesi, FRAP metoduna göre Fe3+-TPTZ indirgeme kapasitesi, bipiridil reaktifi kullanarak ferröz iyonları (Fe2+) şelatlama aktivitesi, DPPH, ABTS, DMPD radikal giderme aktiviteleri ile süperoksit anyon radikalleri (O2.-) giderme aktivitesi gibi farklı biyoanalitikal metotlar kullanıldı. Ayrıca BHA, BHT, α-tokoferol ve troloks referans antioksidan olarak kullanıldı. Bu madde için ABTS radikal giderme IC50 inhibisyon değeri 3.39 μg mL-1 olarak hesaplandı. BHA, α-Tocopherol, trolox, BHT ve gibi standart antioksidanlar, sırasıyla 2.59, 4.44, 7.07 ve 32.36 μg mL-1 olarak ABTS radikal giderme inhibisyonları sergiledi. Çalışmalar 2,6-kinolindiolün, etkili bir antioksidana sahip olduğunu açıkça göstermiştir.

Anahtar Kelimeler:

2.6-kinolindiol, Antioksidan aktivite, Radikal giderme

Determination of Antioxidant Capacity of 2,6-Quinolinediol

The compounds of the quinolone group have been widely used in the alive metabolism, paint and pharmaceutical industry. Therefore, various quinoline derivatives are synthesized and synthesis methods are developed. The synthesis of the quinoline compounds in the industrial field is formed in a separate area. 2,6-quinolinediol is derivative a quinoline. In this study, different bioanalytical methods such as reducing capacity by Fe3+-Fe2+ transformation method, Fe3+-TPTZ reducing capacity by FRAP method, Cu2+-Cu+ reducing capacity by CUPRAC method, the ferric ions (Fe2+) chelating activity by using bipyridyl reagent, DPPH, ABTS, DMPD radical scavenging activities, superoxide anion radicals (O2.-) scavenging activity have been used. Also, Trolox, α-Tocopherol, BHA and BHT have been used as reference antioxidants. The IC50 inhibition value of the ABTS radical removal activity for this substance was calculated as 3.39 μg mL-1. Reference antioxidants such as trolox, BHA, α-Tocopherol and BHT exhibited ABTS radical removal inhibitions at 2.59, 4.44, 7.07 and 32.36 μg mL-1, respectively. Studies have shown that 2,6-quinolinediol is an effective antioxidant.

Keywords:

Antioxidant activity, Radical scavenging, 2.6-quinolinediol,

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