Cerebrospinal fluid and plasma free amino acid levels in multilple sclerosis

Multipl sklerozun (MS) patofizyolojisiyle bazı peptidler ve aminoasitlerin ilişkili olduğu öne sürülmüştür. Dolayısıyla, beyin omurilik sıvısı (BOS) ve plazma amino asit (a.a) düzeylerini MS hastalarında ve sağlıklı bireylerde incelemeyi ve iki grubu karşılaştırmayı amaçladık. 10 MS hastası ile 10 sağlıklı birey çalışmaya alındı. Plazma ve BOS a.a düzeyleri Pickering post kolon derivatizasyon HPLC yöntemiyle tayin edildi. BOS için, total, esansiyel, glukoketojenik, standart ve türev a.a miktarları MS ve kontrol grupları arasında anlamlı farklılık göstermezken, nonesansiyel (p

Multipl sklerozda beyin omirilik sıvısı ve plazma serbest aminoasit düzeyleri

Some peptides and excitatory amino acids are claimed to be involved in the pathophysiology of multiple sclerosis (MS). Thus, we aimed to investigate cerebrospinal fluid (CSF) and plasma amino acid (a.a.) levels in MS patients and healthy subjects and to compare the two groups of subjects in terms of a.a content of CSF and plasma. Ten MS patients and 10 healthy subjects were included in this study. Plasma and CSF a.a. levels were determined by HPLC method with Pickering post-column derivatization. For CSF, while there was no significant difference between MS and control groups in terms of total, essential, glucoketogenic, standard and derivated a.a. contents, nonessential (p<0.05) and glucogenic (p<0.05) a.a. contents were found higher in the patients. For plasma, while no significant difference was present with respect to essential, glucoketogenic and derivated a.a. contents, total (p<0.001), nonessential (p<0.001), glucogenic (p<0.001) and standard (p<0.001) a.a. contentswere found increased in MS patients. Plasma/CSF a.a. ratios were 1.51 and 1.99 in the control and MS groups, respectively. MS patients had significantly higher CSF Thr, Gln, Tyr and Phe and plasma Thr, Ser, Gln, and Gly levels, and plasma/CSF a.a. ratio when compared to healthy controls.

Kaynakça

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