ERKEN VE GEÇ BAŞLANGIÇLI ALZHEIMER HASTALARININ PERİFERİK KANLARINDA SEÇİLMİŞ miRNA’LARIN ANLATIM DÜZEYİ

Amaç: Enflamasyon ve ilişkili mikro RNA’lar (miRNA), Alzheimer hastalığının (AH) patogenezinde önemli rol oynamaktadırlar. AH beyininde görülen miRNA’ların anlatım profilindeki değişikliklerin periferal kan mononükleer hücrelerine de yansımış olması, AH sırasında görülen enflamatuar değişikliklerin bir göstergesi olabilir. Bu çalışmada, Alzheimer hastalarının hem periferal lökositlerinde hem de plazmalarında belirli enflamatuvar miRNA’ların (mir-146a, mir-144, mir-34a) anlatım düzeylerindeki değişiklikleri araştırmayı ve ve AH tanısında biyobelirteç olarak potansiyel kullanılabilirliklerini değerlendirmek. Ayrıca, bu miRNA’ların anlatımlarının erken ve geç başlangıçlı AH arasında farklılık gösterip göstermediğini belirlemeyi amaçladık. Gereç ve Yöntem: Gerçek zamanlı polimeraz zincir reaksiyonu yöntemini kullanarak 16 erken başlangıçlı ve 26 geç başlangıçlı AH hastası ve yaş-uyumlu kontrollerinde miRNA’ların anlatım düzeylerini araştırdık. Bulgular: Plazma mir-144 anlatım düzeyinin erken ve geç başlangıçlı AH hastaları arasında anlamlı farklılık gösterdiğini saptadık (p = 0.015). Ek olarak, lökosit mir-34a anlatım düzeyi erken başlangıçlı AH’de geç başlangıçlı AH hastalarına göre anlamlı derecede düşük bulundu (p = 0.027). Sonuçlarımız ayrıca, yaş ve plazma mir-144 (r=0.319, p=0.02) ile lökosit mir-34a (r=0.414, p=0.001) anlatım düzeyleri arasında anlamlı pozitif korelasyon olduğunu gösterdi. Sonuç: Bulgularımız erken ve geç başlangıçlı AH hastaları arasında plazma mir-144 ve lökosit mir-34a anlatımlarında görülen farklılığın miRNA ekspresyonundaki yaşa bağlı değişiklikleri yansıttığını ve hastalık durumundan bağımsız olabileceğini düşündürmektedir.

EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS

Objective: Inflammation and associated microRNAs (miRNA) both play essential roles in the pathogenesis of Alzheimer’s disease (AD). The expression profile of miRNA’s in an AD brain, also reflected in the peripheral blood mononuclear cells, may give support to the inflammatory changes seen in the course of AD. We aimed to investigate the expression levels of specific inflammatory miRNAs (mir-146a, mir-144, mir-34a) in both blood leukocytes and plasma of AD patients and to evaluate their potential usability as biomarkers in AD diagnosis and to also demonstrate whether the expression of these miRNAs differ between early and late-onset AD patients. Methods: We investigated the expression levels of miRNAs in 16 early-onset and 26 late-onset AD patients and in their respective controls by using qRT-PCR. Results: Plasma mir-144 levels were significantly different between EOAD and LOAD patients (p=0.015). In addition, levels of leukocyte mir-34a were significantly down-regulated in EOAD compared to LOAD patients (p=0.027). Our results also showed significant positive correlations between age and plasma mir- 144 and leukocyte mir-34a expressions. Conclusion: The differential expression of plasma mir-144 and leukocyte mir-34a between EOAD and LOAD patients might reflect age-dependent changes in miRNA expression and might be independent of the disease status.

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Kaynak Göster

Bibtex @araştırma makalesi { iuitfd894678, journal = {Journal of Istanbul Faculty of Medicine}, issn = {}, eissn = {1305-6441}, address = {}, publisher = {İstanbul Üniversitesi}, year = {2021}, volume = {84}, pages = {165 - 174}, doi = {10.26650/IUITFD.2020.0061}, title = {EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS}, key = {cite}, author = {Güven, Gamze and Lohmann, Ebba and Güleç, Çağrı and Bilgiç, Başar and Özer, Ebru and Hanağası, Haşmet and Gürvit, Hakan and Ünaltuna, Nihan} }
APA Güven, G , Lohmann, E , Güleç, Ç , Bilgiç, B , Özer, E , Hanağası, H , Gürvit, H , Ünaltuna, N . (2021). EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS . Journal of Istanbul Faculty of Medicine , 84 (2) , 165-174 . DOI: 10.26650/IUITFD.2020.0061
MLA Güven, G , Lohmann, E , Güleç, Ç , Bilgiç, B , Özer, E , Hanağası, H , Gürvit, H , Ünaltuna, N . "EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS" . Journal of Istanbul Faculty of Medicine 84 (2021 ): 165-174 <https://dergipark.org.tr/tr/pub/iuitfd/issue/62206/894678>
Chicago Güven, G , Lohmann, E , Güleç, Ç , Bilgiç, B , Özer, E , Hanağası, H , Gürvit, H , Ünaltuna, N . "EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS". Journal of Istanbul Faculty of Medicine 84 (2021 ): 165-174
RIS TY - JOUR T1 - EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS AU - Gamze Güven , Ebba Lohmann , Çağrı Güleç , Başar Bilgiç , Ebru Özer , Haşmet Hanağası , Hakan Gürvit , Nihan Ünaltuna Y1 - 2021 PY - 2021 N1 - doi: 10.26650/IUITFD.2020.0061 DO - 10.26650/IUITFD.2020.0061 T2 - Journal of Istanbul Faculty of Medicine JF - Journal JO - JOR SP - 165 EP - 174 VL - 84 IS - 2 SN - -1305-6441 M3 - doi: 10.26650/IUITFD.2020.0061 UR - https://doi.org/10.26650/IUITFD.2020.0061 Y2 - 2020 ER -
EndNote %0 İstanbul Tıp Fakültesi Dergisi EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS %A Gamze Güven , Ebba Lohmann , Çağrı Güleç , Başar Bilgiç , Ebru Özer , Haşmet Hanağası , Hakan Gürvit , Nihan Ünaltuna %T EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS %D 2021 %J Journal of Istanbul Faculty of Medicine %P -1305-6441 %V 84 %N 2 %R doi: 10.26650/IUITFD.2020.0061 %U 10.26650/IUITFD.2020.0061
ISNAD Güven, Gamze , Lohmann, Ebba , Güleç, Çağrı , Bilgiç, Başar , Özer, Ebru , Hanağası, Haşmet , Gürvit, Hakan , Ünaltuna, Nihan . "EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS". Journal of Istanbul Faculty of Medicine 84 / 2 (Nisan 2021): 165-174 . https://doi.org/10.26650/IUITFD.2020.0061
AMA Güven G , Lohmann E , Güleç Ç , Bilgiç B , Özer E , Hanağası H , Gürvit H , Ünaltuna N . EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS. İst Tıp Fak Derg. 2021; 84(2): 165-174.
Vancouver Güven G , Lohmann E , Güleç Ç , Bilgiç B , Özer E , Hanağası H , Gürvit H , Ünaltuna N . EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS. Journal of Istanbul Faculty of Medicine. 2021; 84(2): 165-174.
IEEE G. Güven , E. Lohmann , Ç. Güleç , B. Bilgiç , E. Özer , H. Hanağası , H. Gürvit ve N. Ünaltuna , "EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS", Journal of Istanbul Faculty of Medicine, c. 84, sayı. 2, ss. 165-174, Nis. 2021, doi:10.26650/IUITFD.2020.0061

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