Feridun AKKAFA, Mehmet ARSLAN, Serdar ÖZTUZCU, H. İlyas ÖZARDALI, Avni GÖKALP, Esma ÖZKARA, Celalettin CAMCI, Alper AYTEKİN

Meme Kanserlerinde HtrA2 Gen İfadesinin Analizi

Amaç: Bu çalışmada meme kanserli hastaların tümörlü dokularında ve çevresindeki normal dokularında, High temprature requirement factor A2 HtrA2 geninin hem mRNA hem de protein ifade seviyelerinin tespitini amaçladık.Materyal ve metod: Cerrahi olarak 53 hastadan alınan dokularda mRNA ifade seviyelerinin tespiti qRTPCR ile taze dokularda çalışıldı. Aynı hastalara ait parafine gömülü dokularda ise immünohistokimyasal yöntemle protein tespiti yapıldı. HtrA2 ifade seviyeleri ile hastaların klinik ve patolojik özellikleri arasındaki ilişkiler analiz edildi.Bulgular: Tümörlü dokulardaki HtrA2 geni mRNA ifade seviyeleri, bitişik normal dokulardakiler ile karşılaştırıldığında istatistiksel olarak anlamlı bir fark bulunmadı p=0.074 . HtrA2 proteini, %90.6 hastanın 48/53 tümör dokusunda pozitif olarak tespit edilirken, %9.4 hastanın 5/53 tümör dokusunda ise tespit edilmedi. Tümörlü dokulardaki HtrA2 protein ifadesi, bitişik normal dokulardakiler ile karşılaştırıldığında istatistiksel olarak anlamlı bir fark bulundu p=0.007 .Sonuç: Meme tümör dokularında, HtrA2'nin farklı ifade seviyelerinde tespit edilmesi ile apoptotik yolağı etkinleştirebileceği söz konusu olabilir. Bu nedenle meme kanserinde özellikle IAP'lerin ifade seviyelerinin de tespit edilmesinin önemli olacağını düşünmekteyiz

Anahtar Kelimeler:

HtrA2, qRT-PCR, immunohistokimya, meme kanseri, apoptozis

Analysis of HtrA2 Gene Expression in Breast Cancers

Background: The purposes of this study were to measure both the protein and mRNA expression levels of HtrA2 gene in human with breast cancer tissues and their adjacent normal breast tissues. Methods: mRNA expression levels in tissues taken surgically from 53 patients detection of fresh tissues were studied by qRT-PCR. The protein detection is done in paraffin-embedded tissues of the same patients through immunohistochemical method. The relationships between patients clinical, pathologic features and HtrA2 expression levels are analyzed. Results: Expression levels of HtrA2 mRNA in tumor tissues was not significantly different statically compared with adjacent normal tissues p=0.074 . While HtrA2 protein level is to identified positively in tumor tissue of patients 90.6% 48/53 , there is no detection of HtrA2 protein expression in tumor tissues of patients 9.4% 5/53 . Expression of HtrA2 protein in tumor tissues was significantly different statically compared with adjacent normal tissues p=0.007 .Conclusions: It is possible that apoptotic patway may be activated by the different expression levels of HtrA2 in breast tumor tissues. Therefore, we concluded that it is particularly important to detect the expression levels of IAPs in breast cancer

Keywords:

HtrA2, qRT-PCR, immunohistochemistry, breast cancer, apoptosis,

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