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Salep tozunun bifidojenik etkisi

Bu araştırmanın amacı, salep tozunun B. lactis, B. infantis, B. longum ve B. bifidum’u içeren bazı Bifidobacterium türleri üzerine bifidojenik etkisini araştırmaktır. Bifidobacterium türlerinin gelişmesi, 48 saatlik fermantasyon süresince pH ve optik yoğunluk (OD) ölçülerek incelenmiştir. Bifidobacterium türleri tarafından salep tozunun fermantasyonunun etkinliğini değerlendirmek amacıyla gelişme ortamındaki laktik, asetik, bütirik ve propiyonik asit değerleri HPLC cihazı kullanılarak saptanmıştır. Bifidobacterium türlerinin karbon kaynağı olarak salep tozunu kullanabildiği ve glikoz içeren ortamda B. lactis ve salep tozu içeren ortamda B. infantis için en yüksek OD değerleri gözlenmiştir. Test edilen tüm suşların, propiyonik ve bütirik asit miktarlarından daha yüksek oranda laktik ve asetik asit üretebildiği belirlenmiştir. Sonuç olarak, dört Bifidobacterium türünün salep tozunu fermente edebildiği gözlenmiştir. Ayrıca, salep tozu Bifidobacterium türlerinin gelişmesini ve aktivitesini artırdığı için bifidojenik etkili olarak kabul edilebilir.

Anahtar Kelimeler:

Bifidobacterium, Bifidojenik etki, Salep tozu

Bifidogenic effect of salep powder

The aim of this research was to study the bifidogenic effect of salep powder on some Bifidobacterium species, namely B. lactis, B. infantis, B. longum and B. bifidum. The growth of the Bifidobacterium species was investigated by measuring the pH and optical density (OD) during 48 h-fermentation. In order to evaluate the efficiency of the fermentation of salep powder by the Bifidobacterium subsp. lactic, acetic, butyric and propionic acid values in the growth media were quantified by HPLC. It was determined that Bifidobacterium species might be used salep powder as a source of carbon and the highest OD values were found for B. lactis in media containing glucose and for B. infantis in media containing salep powder. All the tested strains observed the ability to produce higher amounts of lactic and acetic acids than amounts of propionic and butyric acids. Consequently, it was determined that all four Bifidobacterium species were able to ferment salep powder. Moreover, because salep powder enhanced the growth and activity of Bifidobacteria and it might be conferred as a bifidogenic effect.

Keywords:

Bifidobacterium, Bifidogenic effect, Salep powder,

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