Senem ŞANLI, Elvan ÖZSOY, Nurullah ŞANLI

Sertralin’in Farmasötik Dozaj Formlarında Yeni, Basit, Çevreci Kapiler Elektroforez ile Tayini

S ertralinin ilaç formülasyonlarından tayini için kolay, seçici, kesin ve ekonomik kapiler elektroforez yöntemi geliştirilmiştir. Tayin 205 nm’de pH’sı 4.0 olan fosfat tamponunda gerçekleştirilmiştir. Geliştirilen yöntemde, doğrusal aralık 1-12 µg.mL-1 arasında bulunmuştur. Korelasyon katsayısı 0.999’dur. Analiz sonuçları istatistiksel olarak valide edilmiş ve geri kazanım çalışmaları yöntemin doğruluğunu onaylamıştır. Yöntemin kesinliği gün içi ve günler arası belirlenen sınırlar içerisinde bulunmuştur RSD

Anahtar Kelimeler:

Psikiyatrik hastalıklar, depresyon, sertralin, kapiler elektroforez

A Novel, Simple and Green Capillary Electrophoresis Determınation of Sertraline in Pharmaceutical Dosage Forms

Asimple, accurate, precise and economical capillary electrophoresis method has been developed for estimation of Sertraline in pharmaceutical dosage form. The estimation of Sertraline was done at 205 nm in pH 4.0 phosphate buffer. In the developed method, linearity over the concentration range of 1-12 μg.mL-1 of Sertraline was observed. The linear regression was found to be 0.999. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method. The precision intra-day & inter-day of method was found within limits RSD

Keywords:

Psychiatric illnesses, depression, sertraline, Capillary electrophoresis,

PDF

___

1. L.L. Judd, Mood disorders in the general population represent an important and worldwide public health problem, Int. Clin. Psychopharmacol., 10 (1995) 5-10.
2. M.J. Burke, S.H. Preskorn, F.E. Bloom, D.J. Kupfer, (eds.), Psychopharmacology: the fourth generation of progress, Raven Press, New York, (1995) 1053.
3. M.E. Bosch, A.J. Ruiz Sanchez, F. Sanchez Rojas, C.B Ojeda, Analytical methodologies for the determination of sertraline, J. Pharm. Biomed. Anal., 48 (2008) 1290–1302.
4. M.X. Zhou, J.P. Foley, Analytical method for the quantitation of sertraline hydrochloride stereoisomers by electrokinetic chromatography, J. Chromatogr. A,15 (2004) 13-23.
5. A. Khraiwesh, I. Papoutsis, P. Nikolaou, C. Pistos, C. Spiliopoulou, S.J. Athanaselis, Development and validation of an EI-GC/MS method for the determination of sertraline and its major metabolite desmethyl-sertraline in blood, J. Chromatogr. B, 879 (2011) 2576–2582.
6. J.P. Lamas, C. Salgado-Petinal, C. Garcia-Jares, M. Llompart, R. Cela, M. Gomez, Solid-phase m i c ro ex tr a c ti o n - g a s c h ro m a to g r a p hy - m a s s spectrometry for the analysis of selective serotonin reuptake inhibitors in environmental water, J. Chromatogr. A, 1046 (2004) 241–247.
7. L.P. Melo, A.M. Nogueira, F.M. Lancas, M.E.C. Queiroz, Polydimethylsiloxane/polypyrrole stir bar sorptive extraction and liquid chromatography (SBSE/LC-UV) analysis of antidepressants in plasma samples, Anal. Chim. Acta, 633 (2009) 57–64
8. A.R. Chaves, F.Z. Leandro, J.A. Carris, M.E.C. Queiroz, Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection, J. Chromatogr. B, 878 (2010) 2123–2129.
9. G. Bahrami, B. Mohammadi, A. Farshchi, G. Ghiasi, Quantitative analysis of sertraline in human serum by LC with fluorescence detection after pre-column derivatization with 4-chloro-7-nitrobenzofurazan, Chromatographia, 70 (2009) 323–327.
10. N.Y. Khalil, A.M. Mahmoud, I.A. Darwish, A.A. Al-Majed, Highly sensitive LC method with automated co-sense system and fluorescence detection for determination of sertraline in human plasma, Chromatographia, 71 (2010) 825–831.
11. M.M. Zheng, S.T. Wang, W.K. Hu, Y.Q. Feng, Intube solid-phase microextraction based on hybrid silica monolith coupled to liquid chromatography– mass spectrometry for automated analysis of ten antidepressants in human urine and plasma, J. Chromatogr. A, 1217 (2010) 7493–7501.
12. J.M. Conley, S.J. Symes, S.A. Kindelberger, S.M. Richards, Rapid liquid chromatography-tandem mass spectrometry method for the determination of a broad mixture of pharmaceuticals in surface water, J. Chromatogr. A, 1185 (2008) 206–215.
13. Guideline, ICH Harmonized Tripartite, Validation of analytical procedures: text and methodology, Q2 (R1) 2005; Available from URL: http://www.ich. org/products/guidelines/quality/article/qualityguidelines.html