Spinal kord travma modelinde aminoguanidin ile N-Asetilsistein'in ayrı ayrı ve birlikte nöroprotektif etkilerinin incelenmesi

Çalısmada omurilik yaralanmasından sonra verilen N-Asetilsistein ve Aminoguanidin'in nöroprotektif etkinliği klinik ve laboratuar olarak incelenmiştir. Ratlar; kontrol grubu, travma grubu, Aminoguanidin grubu, N-Asetilsistein grubu, Aminoguanidin+N-Asetilsistein grubu olmak üzere 5 gruba ayrılmıştır. Tüm ratlara Torakal 9 veya Torakal10 laminasına total laminektomi yapıldı. Kontrol grubu dışındaki gruplara ağırlık düşürme modeli ile spinal travma oluşturuldu. Travma grubuna; günde 2 kez 1mL %0.9'luk NaCl çözeltisi, Aminoguanidin grubuna; günde 2 kez 100 mg/kg, N-Asetilsistein grubuna; günde 2 kez 100 mg/kg 5 gün boyunca uygulandı. Aminoguanidin+N-Asetilsistein grubuna; her iki ilaç aynı pozolojide 5 gün boyunca uygulandı. Tüm ratlar 1. 3. ve 5.gün Tarlov'un tanımladığı nörolojik muayene derecelendirmesi ile Rivlin ve Tator'un tanımladığı eğik düzlem dereceleri ile klinik olarak değerlendirildi. Ratlardan 6. günde travma bölgesini içeren omurilik alındı. Dokulardan malondialdehit düzeyi, superoksid dismutaz, glutatyon peroksidaz aktivitesi bakıldı. Bulguların analizi SPSS 15.0 programıyla yapılmış kontrol grubuna gore Aminoguanidin, N-Asetilsistein, Aminoguanidin+N-Asetilsistein gruplarında klinik nörolojik muayene ve eğik düzlem derecelendirmesinde artış ile malondialdehit düzeyi, süperoksid dismutaz ve glutatyon peroksidaz aktivitelerinde istatistiki anlamlı düşme görüldü. Sonuç olarak Aminoguanidin ve N-Asetilsistein ayrı ayrı ve birlikte kullanıldıklarında ratlarda oluşturulan omurilik yaralanmasında nöroprotektif etkinliği olduğu fakat birlikte kullanıldıklarında additif etkinlik göstermedikleri söylenebilir

The Investigation of Neuroprotective Effects of Aminoguanidin and N-Acetylcystein administration alone and combination in Spinal Cord Trauma mode

In this study the neuroprotective efficacy of N-Acetylcystein and Aminoguanidin administered after spinal cord injury was examined regarding clinical and laboratory outcome. Rats were divided into 5 groups: Control group, trauma group, Aminoguanidin group, N-Acetylcystein group, and Aminoguanidin+N- Acetylcystein group. Total laminectomy of thoracal 9th or 10th vertebrae was performed on all rats. Spinal trauma was formed by weight drop model for all groups except the control group. 1 ml of 0.9% NaCl solution was administered twice daily to control group. 100 mg/kg Aminoguanidin and N-Acetlysistein was given twice daily for 5 days to own group, Both drugs were administered to Aminoguanidin+N-Acetylcystein group in the same dose. Neurological examination results were graded according to Tarlov's chart and all the rats were clinically assesed by Rivlin and Tator's inclined plane degrees. Traumatized spinal cord segments were obtained. Malondialdehyte, superoxide dismutase, glutathion peroxidase activity of these tissue specimens were measured. Results revealed an increase in the inclined plane degrees and neurological examination grading along with statistically significant decrease in Malondialdehyte level, superoxide dismutase and glutathion peroxidase activities in Aminoguanidin, N-Acetylcystein and Aminoguanidin+N-Acetylcystein groups as compared to control group. We propose, Aminoguanidin and N-Acetylcystein can show neuroprotection when used in combination or alone but there is no additive effect of combined use.

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