Zymomonas mobilis LEVANSUKRAZ ENZİMİNİN LEVAN ÜRETİMİNDE KULLANILMASI

Levan gıda, kozmetik ve ilaç sanayi gibi birbirinden farklı endüstrilerde kullanım alanına sahip bir fruktoz polimeridir. Levansukrazlar sakkarozu substrat olarak kullanarak fruktoz polimerleri oluşumunu katalizleyen enzimlerdir. Bakteriler tarafından hücre dışına salgılanan levansukrazlar bakterinin yüksek sakkaroz konsantrasyonunda levan ve/veya fruktooligosakkarit polimerlerini sentezlemesini sağlar. Bu çalışmada Zymomonas mobilis NRRL B-14023 kullanılarak levansukraz enziminin üretilmesi ve levan polimeri üretme koşulları incelenmiştir. Yapılan çalışmada Z. mobilis levansukrazı üretilmiş ve ham enzimin levan aktivitesi gösterilmiştir. Levansukraz, Z. mobilis B-14023 hücreleri tarafından 36 saat boyunca 30.3 °C’de 159 g/L sakkaroz, pH 4.91 ortamında statik kültürde üretilmiştir. Levansukrazın levan üretimi için optimum inkübasyon süresi ve sıcaklığı 24 saat ve 25 °C olarak bulunduktan sonra aktiviteye etki eden faktörler araştırılmıştır. Artan NaCl konsantrasyonunda levan üretiminde azalma olduğu görülmüştür. Ayrıca 10 mM EDTA ve MgCl2 varlığında enzimin levan üretim aktivitesinde azalma olduğu bulunmuştur. Sonuç olarak Z. mobilis NRRL B-14023 levansukrazı ile ortalama 62.42 g/L levan üretilmiştir.

USE of Zymomonas mobilis LEVANSUCRASE IN LEVAN PRODUCTION

Levan has a wide range of uses in different industries such as food, cosmetics and pharmaceutical. Levansucrases are enzymes, which catalyze the formation of fructose polymers using sucrose as substrate. Levansucrases are produced and secreted by bacteria and enable them to synthesize levan/fructooligosaccharides in medium containing high sucrose concentrations. In this study, Zymomonas mobilis levansucrase was produced and its levan activity was investigated. Levansucrase was produced by Z. mobilis NRRL B-14023 cells in static culture in medium with 159 g/L sucrose, pH 4.91 at 30.3 °C for 36 h. The optimum incubation time, temperature of crude enzyme for levan production were found as 24 h and 25 °C. The levan production of levansucrase decreased with increasing NaCl concentrations. Furthermore the presence of 10 mM EDTA and MgCl2 showed an inhibitory effect on levan production. As a result, on average 62.42 g/L levan was produced using Z. mobilis NRRL B-1423 levansucrase.

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Gıda-Cover
  • ISSN: 1300-3070
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1976
  • Yayıncı: Prof. Dr. İbrahim ÇAKIR
Sayıdaki Diğer Makaleler

FARKLI TİP VE DÜZEYLERDE HİDROKOLLOİD KULLANIMININ SUFLE KEK KALİTESİNE ETKİSİ

Büşra ÇINAR, Halef DİZLEK

ÖN İŞLEMSİZ DONMUŞ DEPOLANAN (-22± 1 ºC) HAMSİ (Engraulis encrasicolus, Linnaeus 1758) VE PALAMUT (Sarda sarda, Bloch 1793) BALIKLARININ DUYUSAL, BESİNSEL, KİMYASAL VE MİKROBİYOLOJİK ÖZELLİKLERİ

Bengunur ÇORAPCI

BERGAMUT UÇUCU YAĞININ ARAP ZAMKI VE MALTODEKSTRİN KARIŞIMLARI KULLANILARAK MİKROENKAPSÜLASYONU: UÇUCU YAĞ BİLEŞENLERİNİN STABİLİTE VE SALINIM ÖZELLİKLERİNİN BELİRLENMESİ

Kübra Sultan ÖZDEMİR, Negin AZARABADİ, Ayhan Topuz

HİNDİSTAN CEVİZİ EKSTRAKTI İÇEREN SOĞUK KAHVE İÇECEĞİNİN GELİŞTİRİLMESİ VE RAF ÖMRÜNÜN BELİRLENMESİ

Davut KARAHAN, Nene Meltem KEKLİK

GIDA VE YEMLERDE GENETİK MODİFİYE MISIRIN PCR METOTLARI İLE TESPİTİ

Melike BARAN EKİNCİ, Filiz ÖZÇELİK

Zymomonas mobilis LEVANSUKRAZ ENZİMİNİN LEVAN ÜRETİMİNDE KULLANILMASI

Güler SÖZGEN, Gökçenaz ÖZDOĞAN, Burcu KAPLAN TÜRKÖZ

FARKLI KAYISI ÇEŞİTLERİNDE SUDA ÇÖZÜNÜR BAZI PARAMETRELERİN ARAŞTIRILMASI

Ahmet BAYSAR, Fikret KARATAŞ

KOCAYEMİŞ (DAĞ ÇİLEĞİ-ARBUTUS UNEDO L.) MEYVELİ DONDURMA ÜRETİMİ, FİZİKSEL, KİMYASAL VE DUYUSAL PARAMETRELER AÇISINDAN İRDELENMESİ

Doç.dr. Hatice Şanlidere ALOĞLU, Yeşim GÖKGÖZ, Müzeyyen BAYRAKTAR

OKRATOKSİN A'NIN EKMEKTE BULUNMA MİKTARINDA FERMANTASYON VE PİŞİRMENİN ETKİSİ

İşıl VAR, Özlem ATASEVER SAVAŞ, Selin SAĞLAM, Okşan Uçkun

ATIMLI UV IŞIĞIN BEYAZ ŞAPKALI KÜLTÜR MANTARI (AGARICUS BISPORUS) YÜZEYİNDE LISTERIA MONOCYTOGENES’İN İNAKTİVASYONU ÜZERİNE ETKİLERİ

Gamze KOÇER, Nene Meltem KEKLİK