Bülent BAŞYİĞİT, Mustafa ÇAM, Betül AKYURT

YEDİ YENİLEBİLİR YAPRAĞIN FENOLİK BİLEŞİK İÇERİĞİ, ANTİOKSİDAN VE ANTİDİYABETİK POTANSİYELİ

Bu çalışmanın amacı seçilmiş yedi tane yenilebilir yaprağın etanolik ve sulu ekstrelerinin antioksidan ve enzim inhibitör aktivitelerinin belirlenmesidir. Yaprakların antioksidan kapasiteleri DPPH ve β-Karoten ağartma yöntemleri kullanılarak analiz edilmiştir. Enzim inhibitör aktivite analizinde ise yaprakların α-glukozidaz enzimini inhibe etme özelliği tespit edilmiştir. Yaprakların antioksidan (EC50) ve α-glukozidaz (IC50) aktiviteleri sırasıyla 8.29-238.8 g örnek/g DPPH ve 35.59-620.29 μg/mL arasında bulunmuştur. İncelenen yapraklar arasında, üzüm yaprağı (Vitis vinifera), ayva (Cydonia oblonga) yaprağı ve ısırgan otunun (Urtica dioica) diğer yapraklardan daha yüksek antioksidan ve a-glukosidaz inhibisyon aktivite gösterdiği belirlenmiştir. Yaprakların toplam fenolik madde miktarı (TPC), toplam flavonoid madde miktarı (TFC) ve toplam hidrolize edilebilir tanin miktarı (TTC) sırasıyla 0.22-16.37 mg gallik asit eşdeğeri (GAE), 0.07-9.32 mg kateşin eşdeğeri (CE) ve 0.49-16.38 tannik asit eşdeğeri (TAE)/g örnek arasında olduğu tespit edilmiştir. Bu çalışma, seçilmiş yedi tane yenilebilir yaprağın biyoaktif bileşenleri ile ilgili doğrudan karşılaştırmalı veriler sunmaktadır.

Anahtar Kelimeler:

Antioksidan aktivite, yenilebilir yapraklar, enzim inhibitör aktivitesi, α-glukosidaz aktivitesi

PHENOLIC COMPOUNDS CONTENT, ANTIOXIDANT AND ANTIDIABETIC POTENTIALS OF SEVEN EDIBLE LEAVES

The objectives of this study were to investigate antioxidant and enzyme inhibitory activity of ethanolic and aqueous extracts of seven leaves. The antioxidant activity of the leaves was determined by two different methods: DPPH and β-carotene- bleaching test (βC). The enzyme inhibitory activity was evaluated against α-glucosidase. Antioxidant (EC50) and α-glucosidase (IC50) activity were determined as 8.29-238.8 g of leaf/g of DPPH and 35.59-620.29 μg/mL respectively. Among seven leaves studied, leaf of grape (Vitis vinifera), quince (Cydonia oblonga) and nettle (Urtica dioica) exhibited higher antioxidant and α-glucosidase inhibition activity than the rest of the leaves. Total phenolic content (TPC), total flavonoid content (TFC) and total hydrolyzable tannin content (TTC) of the leaves were between 0.22-16.37 mg gallic acid equivalents (GAE), 0.07-9.32 mg catechin equivalents (CE) and 0.49-16.38 tannic acid equivalent (TAE) per g of leaf, respectively.

Keywords:

Antioxidant activity, edible leaves, enzyme inhibitory activity, α-glucosidase activity,

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