Ayça GEDİKOĞLU, Andrew Douglas CLARKE, İngolf GRUEN

TURUNÇGİL LİFİNDE FLAVONOİDLERİN BELİRLENMESİ VE ÜRETİM METODUNUN TOPLAM FENOLİK MİKTARI, TOPLAM FLAVONOİD MİKTARI, TOPLAM KAROTENOİT MİKTARI VE OKSİJEN RADİKAL SOĞURMA KAPASİTESİNE ETKİSİ

Bu çalışmanın amacı turunçgil kabuğundan elde edilen liflerin ters fazlı yüksek basınçlı sıvı kromatografisi yöntemi ile flavonoidlerinin belirlenmesidir. Ayrıca, bu çalışmada turunçgil kabuklarından lif hazırlanırken kullanılan normal veya sıcak yıkama işlemlerinin toplam fenol miktarı, toplam flavonoid miktarı, toplam karotenoid miktarı ve oksijen radikal soğurma kapasitesi değerine etkisi araştırılmaktadır. Kullanılan HPLC metodu çoklu metoksiflavon ve flavanollerin ayrılmasını sağlayan ve hızlı sonuç veren bir yöntemdir. Kuersetin, kemferol, sinensetin, nobiletin, heptamethoksiflavon ve tangeretin belirlenmiştir. Nobiletin 3.33 mg/g kuru ağırlık ile en yüksek miktarda bulunan flavonoiddir. Sinensetin 1.96 mg/g kuru ağırlık ile, heptametoksiflavon ise 1.24 mg/g kuru ağırlık ile takip etmektedir. Sıcak yıkama veya normal yıkamanın toplam flavonoid içeriği ve toplam karotenoid içeriğine etkisi görülmemiştir (P >0.05). Ancak, toplam fenolik madde ve oksijen radikal soğurma kapasitesi değeri sıcak yıkama yapılan liflerde daha düşük bulunmuştur (P < 0.05).

Anahtar Kelimeler:

Turunçgil, lif, flavonoid, toplam fenol miktari, oksijen radikal soğurma kapasitesi değeri, HPLC

DETERMINATION OF FLAVONOIDS IN CITRUS FIBER AND THE IMPACT OF PROCESSING METHOD ON TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT, TOTAL CAROTENOID CONTENT AND OXYGEN RADICAL ABSORBANCE CAPACITY

The objective of this study is to evaluate the presence of flavonoids in citrus fıber using reverse-phase high-pressure liquid chromatography (RP-HPLC). In this study, we also investigate the effect of regular and hot-washing preparations on total polyphenol content (TPC), total flavonoid content (TFC), oxygen radical absorbance capacity (ORAC) and total carotenoid content (TCC) of citrus fiber. The HPLC method used provided fast results and good separation for polymethoxyflavones and flavonols. Quercetin and kaempferol (flavonols), and sinensetin, nobiletin, heptamethoxyflavone, and tangeretin (polymethoxyflavones) were identified. Nobiletin had the highest concentration with 3.33 mg/g dry weight followed by sinensetin (1.96 mg/g dry weight) and heptamethoxyflavone (1.24 mg/g dry weight), respectively. The TFC and TCC of citrus fiber were unaffected (P >0.05) by the citrus fiber production procedure; however, the TPC and the ORAC values of citrus fiber prepared using hot-washing were significantly lower (P < 0.05).

Keywords:

Citrus, fiber, flavonoid, total polyphenol content, ORAC value, HPLC,

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