Derya DENİZ ŞİRİNYILDIZ, Ecem AYDIN, Yeliz ÖZTÜRK, Tuğçe AVCI, Aslı YILDIRIM, Aslı YORULMAZ

TÜRK PİYASASINDAN TOPLANAN BİTKİSEL YAĞLAR VE MARGARİNLERDE 3-MCPD YAĞ ASİDİ ESTERLERİNİN DÜZEYİ

Çalışmanın amacı Türkiye piyasasında satışa sunulan bitkisel yağlar (fındık yağı, ayçiçek yağı, kanola yağı ve mısır yağı) ve margarinlerde 3-MCPD ester içeriklerinin belirlenmesidir. Çalışmada marketlerden alınan toplam 27 adet margarin ve bitkisel yağ DGF C VI 18 (10) metoduna göre analiz edilmiştir. Bitkisel yağlar arasında en yüksek 3-MCPD ester miktarı (2.49 mg/kg) mısır yağında tespit edilmiş olup, bunu sırasıyla kanola yağı (0.95 mg/kg), ayçiçek yağı (0.79 mg/kg) ve fındık yağı (0.45 mg/kg) izlemiştir. Analiz edilen örnekler arasında en yüksek 3-MCPD ester miktarı margarinlerde (4.54 mg/kg) saptanmıştır. Çalışma sonuçları Türkiye piyasasında yer alan çeşitli bitkisel yağlar ve margarinlerde farklı miktarlarda 3-MCPD esterlerinin bulunabileceğini göstermektedir.

Anahtar Kelimeler:

3-MCPD esterleri, bitkisel yağ, margarin, pazar araştırması

THE LEVEL OF 3-MCPD FATTY ACID ESTERS IN VEGETABLE OILS AND MARGARINES COLLECTED FROM TURKISH MARKET

The aim of the present research was to investigate the content of 3-MCPD esters in vegetable oils (hazelnut, sunflower, rapeseed and corn oil) and margarines sold out in Turkish local markets. A series of 27 margarines and vegetable edible oil collected from markets were analyzed in the study according to DGF C VI 18 (10) method. Among vegetable oils, corn oil presented highest amount of 3-MCPD esters (2.49 mg/kg) followed by rapeseed oil (0.95 mg/kg), sunflower oil (0.79 mg/kg) and hazelnut oil (0.45 mg/kg), respectively. Highest amount of 3-MCPD esters was detected for margarines (4.54 mg/kg) among analyzed samples. The results demonstrate that 3-MCPD esters can occur at different levels in various vegetable oils and margarines available on Turkish markets.

Keywords:

3-MCPD esters, margarine, market survey, vegetable oil,

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Abraham, K., Appel, K. E., Berger-Preiss, E., Apel, E., Gerling, S., Mielke, H., Creutzenberg, O., Lampen, A. (2013). Relative oral bioavailability of 3-MCPD from 3-MCPD fatty acid esters in rats. Archives of Toxicology, 87(4): 649-659, doi:10.1007/s00204-012-0970-8.
Appel, K. E., Abraham, K., Berger-Preiss, E., Hansen, T., Apel, E., Schuchardt, S., Vogt, C., Bakhiya, N., Lampen, A. (2013). Relative oral bioavailability of glycidol from glycidyl fatty acid esters in rats. Archives of Toxicology, 87(9): 1649–1659, doi:10.1007/s00204-013-1061-1.
Bakhiya, N., Abraham, K., Gürtler, R., Appel, K. E., Lampen, A. (2011). Toxicological assessment of 3‐chloropropane‐1, 2‐diol and glycidol fatty acid esters in food. Molecular Nutrition & Food Research, 55(4): 509-521, doi:10.1002/mnfr.201000550.
Becalski, A., Feng, S., Lau, B. P., & Zhao, T. (2015). A pilot survey of 2-and 3-monochloropropanediol and glycidol fatty acid esters in foods on the Canadian market 2011–2013. Journal of Food Composition and Analysis, 37, 58-66, doi:10.1016/j.jfca.2014.09.002.
Beljaars, P. R., Houwen-Claassen, A. A. (1994). Determination of polymerized triglycerides in frying fats and oils by gel permeation chromatography: interlaboratory study. Journal of AOAC International, 77(3): 667-671.
Bockisch M. 1993. Nahrungsfette und-öle, Handbuch der Lebensmitteltechnologie. Eugen Ulmer GmbH & Co, Stuttgart Hohenheim.
Breitling-Utzmann, C. M., Hrenn, H., Haase, N. U., Unbehend, G. M. (2005). Influence of dough ingredients on 3-chloropropane-1, 2-diol (3-MCPD) formation in toast. Food Additives and Contaminants, 22(2): 97-103, doi:10.1080/02652030500037936.
Calta, P., Velíšek, J., Doležal, M., Hasnip, S., Crews, C., Réblová, Z. (2004). Formation of 3-chloropropane-1, 2-diol in systems simulating processed foods. European Food Research and Technology, 218(6): 501-506, doi:10.1016/j.fct.2008.07.003.
Chung, C., Chan, S. W., Chung, B. T., Xiao, Y., Ho, Y. Y. (2013). Occurrence of bound 3-monochloropropan-1, 2-diol content in commonly consumed foods in Hong Kong analysed by enzymatic hydrolysis and GC-MS detection. Food Additives & Contaminants: Part A, 30(7): 1248-1254, doi:10.1080/19440049.2013.800996.
Craft, B. D., Nagy, K., Seefelder, W., Dubois, M., Destaillats, F. (2012). Glycidyl esters in refined palm (Elaeis guineensis) oil and related fractions. Part II: practical recommendations for effective mitigation. Food Chemistry, 132(1): 73-79, doi:10.1016/j.foodchem.2011.10.034.
Crews C. 2012. Fatty acid esters of chloropropanols and glycidol in foods – analysis and exposure. 1–29.
Crews, C., Brereton, P., Davies, A. (2001). The effects of domestic cooking on the levels of 3-monochloropropanediol in foods. Food Additives & Contaminants, 18(4): 271-280, doi:10.1080/02652030120064.
Davidek, J., Velíšek, J., Kubelka, V., Janíček, G., Šimicová, Z. (1980). Glycerol chlorohydrins and their esters as products of the hydrolysis of tripalmitin, tristearin and triolein with hydrochloric acid, Zeitschrift für Lebensmittel-Untersuchung und Forschung, 171(1): 14-17. doi:10.1007/BF01044410.
Destaillats, F., Angers, P. (2005). On the mechanisms of cyclic and bicyclic fatty acid monomer formation in heated edible oils. European Journal of Lipid Science and Technology, 107(10): 767-772, doi:10.1002/ejlt.200501159.
Destaillats, F., Craft, B. D., Dubois, M., Nagy, K. (2012). Glycidyl esters in refined palm (Elaeis guineensis) oil and related fractions. Part I: Formation mechanism. Food Chemistry, 131(4): 1391-1398, doi:10.1016/J.FOODCHEM.2011.10.006.
Deutsche Gesellschaft für Fettwissenschaft (DGF). 2010. DGF-Standard method C-VI 18 (10) Fatty-acid-bound 3-chloropropane-1,2-diol (3-MCPD) and 2,3-epoxi-propane-1-ol (glycidol) Determination in oils and fats by GC/MS.
Dijkstra AJ, Segers JC. 2007. Production and refining of oils and fats. In The Lipid Handbook with CD-ROM, Third Edition (pp. 150-269). CRC Press.
Doležal, M., Chaloupská, M., Divinová, V., Svejkovská, B., Velišek, J. (2005). Occurrence of 3-chloropropane-1, 2-diol and its esters in coffee. European Food Research and Technology, 221(3-4): 221-225, doi:10.1007/s00217-004-1118-8.
Ermacora, A., Hrncirik, K. (2013). A novel method for simultaneous monitoring of 2‐MCPD, 3‐MCPD and glycidyl esters in oils and fats. Journal of the American Oil Chemists' Society, 90(1): 1-8, doi:10.1007/s11746-012-2132-9.
Franke, K., Strijowski, U., Fleck, G., Pudel, F. (2009). Influence of chemical refining process and oil type on bound 3-chloro-1, 2-propanediol contents in palm oil and rapeseed oil. LWT-Food Science and Technology, 42(10): 1751-1754, doi:10.1016/j.lwt.2009.05.021.
Haines, T. D., Adlaf, K. J., Pierceall, R. M., Lee, I., Venkitasubramanian, P., Collison, M. W. (2011). Direct determination of MCPD fatty acid esters and glycidyl fatty acid esters in vegetable oils by LC–TOFMS. Journal of the American Oil Chemists' Society, 88(1): 1-14, doi:10.1007/s11746-010-1732-5.
Hamlet, C. G., Sadd, P. A., Crews, C., Velíšek, J., Baxter, D. E. (2002). Occurrence of 3-chloro-propane-1, 2-diol (3-MCPD) and related compounds in foods: a review. Food Additives & Contaminants, 19(7): 619-631, doi:10.1080/0265203021013239.
Hamlet, C. G., Sadd, P. A. (2004). Chloropropanols and their esters in cereal products. Czech Journal of Food Sciences, 22(1): 259.
Hrncirik, K., van Duijn, G. (2011). An initial study on the formation of 3‐MCPD esters during oil refining. European Journal of Lipid Science and Technology, 113(3): 374-379, doi:10.1002/ejlt.201000317.
Jędrkiewicz, R., Głowacz, A., Gromadzka, J., Namieśnik, J. (2016). Determination of 3-MCPD and 2-MCPD esters in edible oils, fish oils and lipid fractions of margarines available on Polish market. Food Control, 59: 487-492, doi:10.1016/j.foodcont.2015.05.039.
Karl, H., Merkle, S., Kuhlmann, J., Fritsche, J. (2016). Development of analytical methods for the determination of free and ester bound 2‐, 3‐MCPD, and esterified glycidol in fishery products. European Journal of Lipid Science and Technology, 118(3): 406-417, doi:10.1002/ejlt.201400573.
Kuhlmann, J. (2011). Determination of bound 2, 3‐epoxy‐1‐propanol (glycidol) and bound monochloropropanediol (MCPD) in refined oils. European Journal of Lipid Science and Technology, 113(3): 335-344, 335–344. doi:10.1002/ejlt.201000313.
Lee, B. Q., Khor, S. M. (2015). 3‐Chloropropane‐1, 2‐diol (3‐MCPD) in soy sauce: A review on the formation, reduction, and detection of this potential carcinogen. Comprehensive Reviews in Food Science and Food Safety, 14(1): 48-66, doi:10.1111/1541-4337.12120.
MacMahon, S., Begley, T. H., Diachenko, G. W. (2013). Occurrence of 3-MCPD and glycidyl esters in edible oils in the United States. Food Additives & Contaminants: Part A, 30(12): 2081-2092, 2081–2092. doi:10.1080/19440049.2013.840805.
Raczyk, M., Bonte, A., Matthäus, B., Rudzińska, M. (2018). Impact of Added Phytosteryl/Phytostanyl Fatty Acid Esters on Chemical Parameters of Margarines upon Heating and Pan‐Frying. European Journal of Lipid Science and Technology, 120(2): 1700281, doi:10.1002/ejlt.201700281.
Razak, R. A. A., Kuntom, A., Siew, W. L., Ibrahim, N. A., Ramli, M. R., Hussein, R., Nesaretnam, K. (2012). Detection and monitoring of 3-monochloropropane-1, 2-diol (3-MCPD) esters in cooking oils. Food Control, 25(1): 355-360, doi:10.1016/j.foodcont.2011.10.058.
Schilter, B., Scholz, G., Seefelder, W. (2011). Fatty acid esters of chloropropanols and related compounds in food: Toxicological aspects. European Journal of Lipid Science and Technology, 113(3): 309-313, doi:10.1002/ejlt.201000311.
Velíšek, J., Davidek, J., Hajšlová, J., Kubelka, V., Janíček, G., Mánková, B. (1978). Chlorohydrins in protein hydrolysatesChlorhydrinein Eiweißhydrolysaten. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 167(4): 241-244, doi:10.1007/BF01135595.
Weißhaar, R. (2008). Determination of total 3‐chloropropane‐1, 2‐diol (3‐MCPD) in edible oils by cleavage of MCPD esters with sodium methoxide. European Journal of Lipid Science and Technology, 110(2): 183-186, doi:10.1002/ejlt.200700197.
Weißhaar, R., Perz, R. (2010). Fatty acid esters of glycidol in refined fats and oils. European Journal of Lipid Science and Technology, 112(2): 158-165, doi: 10.1002/ejlt.200900137.
Weißhaar, R. (2011). Fatty acid esters of 3‐MCPD: Overview of occurrence and exposure estimates. European Journal of Lipid Science and Technology, 113(3): 304-308, doi:10.1002/ejlt.200900137.
Wöhrlin, F., Fry, H., Lahrssen-Wiederholt, M., Preiß-Weigert, A. (2015). Occurrence of fatty acid esters of 3-MCPD, 2-MCPD and glycidol in infant formula. Food Additives & Contaminants: Part A, 32(11): 1810-1822, doi:10.1080/19440049.2015.1071497.
Zelinková, Z., Svejkovská, B., Velíšek, J., Doležal, M. (2006). Fatty acid esters of 3-chloropropane-1, 2-diol in edible oils. Food Additives and Contaminants, 23(12): 1290-1298, doi:10.1080/02652030600887628.