SUCUKTAN İZOLE EDİLEN KOAGÜLAZ-NEGATİF

Anahtar Kelimeler:

Enterotoksin genleri, koagülaz-negatif Staphylococcus, Macrococcus caseolyticus, sucuk, multipleks-PZR

STAPHYLOCOCCUS VE MACROCOCCUS CASEOLYTICUS

The aim of this study was to investigate the presence of enterotoxin structural genes in 51 coagulasenegative Staphylococcus (CNS) and 10 Macrococcus caseolyticus strains isolated from traditionalfermented Turkish sausages (sucuk) produced without using starter culture. For this purpose, thepresence of 18 enterotoxin structural genes (sea, seb, sec, sed, see, seg, she, sei, selj, selk, sell, selm, seln,selo, selp, selq, selr and tst1) were researched by polymerase chain reaction (PCR) in a total of 61 isolatesfrom sucuk. The PCR results indicated that none of the isolates has contained enterotoxin structural gene.The results of this study showed that CNS and M. caseolyticus strains isolated from sucuk were safe interms of enterotoxin production

Keywords:

Enterotoxin genes, coagulase-negative Staphylococcus, Macrococcus caseolyticus, Turkishdry fermented sausage (sucuk), multiplex-PCR,

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