STREPTOCOCCUS THERMOPHILUS 231-X10 FAJININ KISMİ GENOMİK KARAKTERİZASYONU

Bu çalışmada, daha önce yapılmış çalışmalarla morfolojik olarak tanımlanmış, konakçı spektrumu ve yapısal proteinleri belirlenmiş, restriksiyon fragment analizleri yapılmış 231-X10 fajının belirli bir genom bölgesinin dizi analizi yapılmıştır. Dizi analizi yapılmış olan bu bölgede 13 adet açık okuma çerçevesinin yani 13 farklı fonksiyonu kodlayan gen bölgesinin olabileceği ortaya çıkarılmıştır. Bu gen bölgelerinin faj DNA’sının replikasyonu, DNA’nın paketlenmesi, kapsid ve kuyruk yapısının oluşumundan sorumlu proteinleri kodladığı belirlenmiştir. 231-X10 fajına ait bu genom bölgesi tarafından kodlanan proteinlerin aminoasit bazında cos-tipi DNA paketleme mekanizmasına sahip fajlarla yüksek benzerlik gösterdiği tespit edilmiş ve dolayısıyla bu fajın da cos-tipi olabileceği sonucuna varılmıştır. Yapılan bu incelemeler sonucunda S. thermophilus fajlarının ortak bir atadan geldiği, nokta mutasyonlar, küçük eklemeler ve kayıplar ile evrimleştikleri düşüncesi desteklenmiştir.

Anahtar Kelimeler:

S. thermophilus fajı, dizi analizi, genomik organizasyon

PARTIAL GENOMIC CHARACTERIZATION OF STREPTOCOCCUS THERMOPHILUS PHAGE 231-X10

In this study, the partial genomic sequencing of S. thermophilus 231-X10 phage was carried out. 231-X10 was identified morphologically, and its host spectrum, structural proteins and restriction analysis have also been completed in previous studies. Thirteen different open reading frames that was coded by thirteen genes were detected in that partial genome. These genes are responsible for DNA replication, DNA packaging and the formation of capsid and tail structure. The proteins that are encoded by the genomic region of 231-X10 phage have been found to show high similarities between the phages which have cos-type DNA packaging mechanism on the basis of amino acids. Thus, it is concluded that the 231-X10 phage might also be cos-type. As a result of these investigations, it is supported the idea that S. thermophilus phages come from a common ancestor and evolved with point mutations, small additions and deletions.

Keywords:

S. thermophilus phage, sequence analysis, genomic organisation,

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