PROBİYOTİK FIRIN ÜRÜNLERİ ÜRETİM YÖNTEMLERİ

Fonksiyonel ürünler dünya çapında tüketicilerin ilgisini çekmektedir. En bilinen fonksiyonel ürünlerden olan probiyotik gıdalar temelde süt bazlı ürünler olsa da süt bazlı olmayan probiyotik gıdalar da yavaş yavaş fonksiyonel ürünler pazarında değerli bir katkı olarak yer almaktadır. Probiyotik ürün üretirken en önemli konu, ürünün üretiminden tüketimine kadar geçen süre içinde canlı probiyotik sayısının üründe en az 106 kob/g veya kob/ml düzeyinde kalmasını sağlamaktır. Birçok kişi tarafından sevilerek tüketilen fırın ürünlerinin üretiminde uygulanan pişirme işlemi, probiyotik mikroorganizmaların hasar görmesine ve canlı probiyotik sayısının üründen beklenen yararlı etkileri sağlayamayacak düzeylere kadar düşmesine neden olmaktadır. Canlı probiyotik sayısını en üst seviyede tutmak için uygulandığı bilinen yöntemler probiyotiklerin mikroenkapsülasyonu, probiyotiklerin yenilebilir film ve kaplamalara ilave edilmesi ve sporlu probiyotik mikroorganizmaların kullanılmasıdır. Bu derlemede probiyotik fırın ürünü üretmek üzere uygulanan yöntemler, bilimsel literatürde konu ile ilgili yapılmış olan çalışmalarla birlikte sunulmuştur.

PRODUCTION METHODS FOR PROBIOTIC BAKERY PRODUCTS

Functional foods attract consumer interest worldwide. Although probiotic foods -one of the most known functional foods- are predominantly based on milk matrices, non-dairy probiotic foods are slowly emerging as a valuable addition to the functional foods market.  The most important issue when producing probiotic foods is to ensure the number of viable probiotics is at least 106 cfu/g or cfu/ml, from production to consumption. The baking process applied in the production of bakery products, which are consumed enjoyably by many people, can cause significant losses of probiotic viability, such that the product does not deliver the expected health benefits.  Several techniques that have been applied to maintain the number of live probiotics at maximum level are microencapsulation, edible films and coatings and using sporulating probiotic microorganisms. This review describes the methods used for producing probiotic bakery products, with reference to relevant studies in the scientific literature.

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  • Altamirano-Fortoul, R., Moreno-Terrazas, R., Quezada-Gallo, A., Rosell, C.M. (2012). Viability of some probiotic coatings in bread and its effect on the crust mechanical properties. Food Hydrocoll 29:166-174, doi: 10.1016/j.foodhyd.2012.02.015.
  • Anonim (2017). Türk Gıda Kodeksi. Beslenme ve sağlık beyanları yönetmeliği. Ek 2: Hastalık riskinin azaltılmasına, çocukların gelişimi ve sağlığına ilişkin beyanlar dışındaki sağlık beyanları listesi. Gıda Tarım ve Hayvancılık Bakanlığı. 26 Ocak 2017 tarih ve 29960 sayılı Resmî Gazete, Ankara.
  • Arslan-Tontul, S., Erbas, M. (2017). Single and double layered microencapsulation of probiotics by spray drying and spray chilling. LWT-Food Sci Technol 81:160-169, doi: 10.1016/j.lwt.2017.03.060.
  • Arslan-Tontul, S., Erbas, M., Gorgulu, A. (2018). The Use of probiotic-loaded single- and double-layered microcapsules in cake production. Probiotics & Antimicro Prot doi: 10.1007/s12602-018-9467-y.
  • Bampi, G.B., Backes, G.T., Cansian, R.L., Matos Jr, F.E., Ansolin, I.M.A., Poleto, B.C., Corezzolla, L.R., Favaro-Trindade, C.S. (2016). Spray chilling microencapsulation of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis and its use in the preparation of savory probiotic cereal bars. Food Bioprocess Technol 9:1422-1428, doi: 10.1007/s11947-016-1724-z.
  • Bansal, S., Mangal, M., Sharma, S.K., Gupta, R.K. (2016). Non-dairy based probiotics: a healthy treat for intestine. Crit Rev Food Sci Nutr 56:1856-1867, doi: 10.1080/10408398.2013.790780.
  • Călinoiu, L.F., Vodnar, D.C., Precup, G. (2016). The probiotic bacteria viability under different conditions. Bulletin UASVM Food Science and Technology 73(2): 55-60, doi: 10.15835/buasvmcn-fst:12448.
  • Cencic, A., Chingwaru, W. (2010). The role of functional foods, nutraceuticals, and food supplements in intestinal health. Nutrients 2:611-625, doi: 10.3390/nu2060611.
  • Coman, M.M., Cecchini, C., Verdenelli, M.C., Silvi, S., Orpianesi, C., Cresci, A. (2012). Functional foods as carriers for SYNBIO®, a probiotic bacteria combination. Int J Food Microbiol 157:346-352, doi: 10.1016/j.ijfoodmicro.2012.06.003.
  • Corona-Hernandez, R.I., Álvarez-Parrilla, E., Lizardi-Mendoza, J., Islas-Rubio, A.R., de la Rosa, L.A., Wall-Medrano, A. (2013). Structural stability and viability of microencapsulated probiotic bacteria: A Review. Compr Rev Food Sci Food Saf 12:614-628, doi: 10.1111/1541-4337.12030.
  • Côté, J., Dion, J., Burguière, P., Casavant, L., Van Eijk, J. (2013). Probiotics in bread and baked products: A new product category. Cereal Food World 58(6):293-296, doi: 10.1094/CFW-58-6-0293.
  • Cutting, S.M. (2011). Bacillus probiotics. Food Microbiol 28:214-220, doi: 10.1016/j.fm.2010.03.007.
  • De Prisco, A., Mauriello, G. (2016). Probiotication of foods: A focus on microencapsulation tool. Trends Food Sci Technol 48:27-39, doi: 10.1016/j.tifs.2015.11.009.
  • Dianawati, D., Mishra, V., Shah, N.P. (2016) Survival of microencapsulated probiotic bacteria after processing and during storage: A review. Crit Rev Food Sci Nutr, 56(10):1685-1716, doi: 10.1080/10408398.2013.798779.
  • Dias, D.R., Botrel, D.A., Victoria, R., Fernandes, D.B., Borges, S.V. (2017). Encapsulation as a tool for bioprocessing of functional foods. Curr Opin Food Sci 13:31-37, doi: 10.1016/j.cofs.2017.02.001.
  • Ebrahimi, B., Mohammadi, R., Rouhi, M., Mortazavian, A.M., Shojaee-Aliabadi, S., Koushki, M.R. (2018). Survival of probiotic bacteria in carboxymethyl cellulose-based edible film and assessment of quality parameters. LWT-Food Sci Technol 87:54-60, doi: 10.1016/j.lwt.2017.08.066.
  • Erem, F., Küçükçetin, A., Certel, M. (2013). Bacillus türlerinin probiyotik olarak değerlendirilmesi. Gıda 38(4):247-254, doi: 10.5505/gida.2013.32042.
  • Ergin, F., Çomak Göçer, E.M., Aşçı Arslan, A., Küçükçetin, A. (2015). Probiyotikler ile ilgili yasal düzenlemeler. Akademik Gıda 13(3):229-236.
  • Espitia, P.J.P., Batista, R.A., Azeredo, H.M.C., Otoni, C.G. (2016). Probiotics and their potential applications in active edible films and coatings. Food Res Int 90:42-52, doi: 10.1016/j.foodres.2016.10.026.
  • Etchepare, M.D.A., Raddatz, G.C., Cichoski, A.J., Flores, E.M.M., Barin, J.S., Zepka, L.Q., Jacob-Lopes, E., Grosso, C.R.F., De Menezes, C.R. (2016a). Effect of resistant starch (hi-maize) on the survival of Lactobacillus acidophilus microencapsulated with sodium alginate. J Funct Foods 21:321-329, doi: 10.1016/j.jff.2015.12.025 1.
  • Etchepare, M.D.A., Raddatz, G.C., Flores, E.M.M., Zepka, L.Q., Jacob-Lopes, E., Barin, J.S., Grosso, C.R.F., de Menezes, C.R. (2016b). Effect of resistant starch and chitosan on survival of Lactobacillus acidophilus microencapsulated with sodium alginate. LWT-Food Sci Technol 65:511-517, doi: 10.1016/j.lwt.2015.08.039 0.
  • FAO/WHO (2002). Report of a joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. London, Ontario, Canada, April 30 and May 1, 2002.
  • FAO/WHO (2006). Probiotics in Food. Health and nutritional properties and guidelines for evaluation. World Health Organization/Food and Agriculture Organization of the United Nations, Rome.
  • Grahn Håkansson, E., Ekbeck, J. (2006). Thermostable Lactobacillus strains. European Patent, EP 1 996 696 B1.
  • Guimarães, A., Abrunhosa, L., Pastrana, L.M., Cerqueira, M.A. (2018). Edible films and coatings as carrier for living microorganisms: a new strategy towards biopreservation and healthier foods. Compr Rev Food Sci Food Saf 17:594-614, doi: 10.1111/1541-4337.12345.
  • Gülbandılar, A., Okur, M., Dönmez, M. (2017). Fonksiyonel gıda olarak kullanılan probiyotikler ve özellikleri. Türk Bilimsel Derlemeler Dergisi 10(1):44-47.
  • Holkem, A.T., Raddatz, G.C., Nunes, G.L., Cichoski, A.J., Jacob-Lopes, E., Grosso, C.R.F., de Menezes, C.R. (2016). Development and characterization of alginate microcapsules containing Bifidobacterium BB-12 produced by emulsification/internal gelation followed by freeze drying. LWT-Food Sci Technol 71:301-308, doi: 10.1016/j.lwt.2016.04.012 0.
  • Holkem, A.T., Raddatz, G.C., Barin, J.S., Flores, E.M.M., Muller, E.I., Codevilla, C.F., Jacob-Lopes, E., Grosso, C.R.F., de Menezes, C.R. (2017). Production of microcapsules containing Bifidobacterium BB-12 by emulsification/internal gelation. LWT-Food Sci Technol 76:216-221, doi: 10.1016/j.lwt.2016.07.013 0.
  • Jao, C.L., Huang, S.L., Wu, S.C., Kuo-Chiang, H. (2011). The study on SFLAB GanedenBC30 viability on baking products during storage. 11th International Congress on Engineering and Food (ICEF11), Procedia Food Sci 1:1601-1609, doi: 10.1016/j.profoo.2011.09.237.
  • Kamarlı Altun, H., Akal Yıldız, E. (2017). Prebiyotikler ve probiyotiklerin diyabet ile ilişkisi. Türk Yaşam Bilimleri Dergisi 2(1):149-156.
  • Kanmani, P., Lim, S.T. (2013). Development and characterization of novel-probiotic-residing pullulan/starch edible films. Food Chem 141:1041-1049, doi: 10.1016/j.foodchem.2013.03.103.
  • Kechagia, M., Basoulis, D., Konstantopoulou, S., Dimitriadi, D., Gyftopoulou, K., Skarmoutsou, N., Fakiri, E.M. (2013). Health benefits of probiotics: A review. ISRN Nutrition 2013:1-7, doi: 10.5402/2013/481651.
  • Kerry, R.G., Patra, J.K., Gouda, S., Park, Y., Shin, H.S., Das, G. (2018). Benefaction of probiotics for human health. J Food Drug Anal 26:927-939, doi: 10.1016/j.jfda.2018.01.002.
  • Konuray, G., Erginkaya, Z., Koç, G. (2017). Bacillus spp.’nin et ürünlerinde probiyotik olarak kullanımları. Çukurova Tarım Gıda Bil Der 32(2):47-60.
  • Kumar, B.V., Vijayendra, S.V.N., Reddy, O.V.S. (2015). Trends in dairy and non-dairy probiotic products-a review. J Food Sci Technol 52(10):6112-6124, doi: 10.1007/s13197-015-1795-2.
  • Lai, H.M., Lin, T.C. (2006). Bakery products: science and technology. In: Bakery products: science and technology, Hui, Y.H. (ed.), Blackwell Publishing, USA, pp. 3-68.
  • Longoria-García, S., Cruz-Hernández, M.A., Flores-Verástegui, M.I.M., Contreras-Esquivel, J.C., Montañez-Sáenz, J.C., Belmares-Cerda, R.E. (2018). Potential functional bakery products as delivery systems for prebiotics and probiotics health enhancers. J Food Sci Technol 55(3):833-845, doi: 10.1007/s13197-017-2987-8.
  • López de Lacey, A.M., López-Caballero, M.E., Gómez-Estaca, J., Gómez-Guillén, M.C, Montero, P. (2012). Functionality of Lactobacillus acidophilus and Bifidobacterium bifidum incorporated to edible coatings and films. Innov Food Sci Emerg Technol 16:277-282, doi: 10.1016/j.ifset.2012.07.001.
  • Lönner, C. (2005). Probiotic bread and method of its production. European Patent, EP1971231A1.
  • Majeed, M., Majeed, S., Nagabhushanam, K., Natarajan, S., Sivakumar, A., Ali, F. (2016). Evaluation of the stability of Bacillus coagulans MTCC 5856 during processing and storage of functional foods. Int J Food Sci Technol 51:894-901, doi: 10.1111/ijfs.13044.
  • Malmo, C., La Storia, A., Mauriello, G. (2013). Microencapsulation of Lactobacillus reuteri DSM 17938 cells coated in alginate beads with chitosan by spray drying to use as a probiotic cell in a chocolate soufflé. Food Bioprocess Tech 6(3):795-805, doi: 10.1007/s11947-011-0755-8.
  • Mansouripour, S., Esfandiari, Z., Nateghi, L. (2013). The effect of heat process on the survival and increased viability of probiotic by microencapsulation: A review. Ann Biol Res 4(4):83-87.
  • Markowiak, P., Śliżewska, K. (2017). Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients 9(1021):1-30, doi: 10.3390/nu9091021.
  • Martín, M.J., Lara-Villoslada, F., Ruiz, M.A., Morales, M.E. (2015). Microencapsulation of bacteria: a review of different technologies and their impact on the probiotic effects. Innov Food Sci Emerg Technol 27:15-25, doi: 10.1016/j.ifset.2014.09.010.
  • Mohajeri, M.H., La Fata, G., Steinert, R.E., Weber, P. (2018). Relationship between the gut microbiome and brain function. Nutr Rev 76(7):481-496, doi: 10.1093/nutrit/nuy009.
  • Muzzafar, A., Sharma, V. (2018). Microencapsulation of probiotics for incorporation in cream biscuits. J Food Meas Charact 12:2193-2201, doi: 10.1007/s11694-018-9835-z.
  • Nunes, G.L., Etchepare, M.D.A., Cichoski, A.J., Zepka, L.Q., Jacob-Lopes, E., Barin, J.S., Flores, E.M.M., da Silva, C.B., de Menezes, C.R. (2018). Inulin, hi-maize, and trehalose as thermal protectants for increasing viability of Lactobacillus acidophilus encapsulated by spray drying. LWT-Food Sci Technol 89:128-133, doi: 10.1016/j.lwt.2017.10.032 R.
  • Pandey, K. R., Naik, S.R., Vakil, B.V. (2015). Probiotics, prebiotics and synbiotics-a review. J Food Sci Technol 52(12):7577-7587, doi: 10.1007/s13197-015-1921-1.
  • Panghal, A., Janghu, S., Virkar, K., Gat, Y., Kumar, V., Chhikara, N. (2018). Potential non-dairy probiotic products-A healthy approach. Food Biosci 21:80-89, doi: 10.1016/j.fbio.2017.12.003.
  • Pereira, J.O., Soares, J., Sousa, S., Madureira, A.R., Gomes, A., Pintado, M. (2016). Edible films as carrier for lactic acid bacteria. LWT-Food Sci Technol 73:543-550, doi: 10.1016/j.lwt.2016.06.060.
  • Permpoonpattana, P., Hong, H.A., Khaneja, R., Cutting, S.M. (2012). Evaluation of Bacillus subtilis strains as probiotics and their potential as a food ingredient. Benef Microbes 3(2):127-135, doi: 10.3920/BM2012.0002.
  • Rampelli, S., Candela, M., Severgnini, M., Biagi, E., Turroni, S., Roselli, M., Carnevali, P., Donini, L., Brigidi, P. (2013). A probiotics-containing biscuit modulates the intestinal microbiota in the elderly. J Nutr Health Aging 17(2):166-172, doi: 10.1007/s12603-012-0372-x.
  • Rasmussen, B., Tolstoy, A. (1992). Baked product containing viable microorganisms and process for preparing same. WO1994000019A1.
  • Romano, N., Tavera-Quiroz, M.J., Bertola, N., Mobili, P., Pinotti, A., Gómez-Zavaglia, A. (2014). Edible methylcellulose-based films containing fructo-oligosaccharides as vehicles for lactic acid bacteria. Food Res Int 64:560-566, doi: 10.1016/j.foodres.2014.07.018.
  • Sanders M.E., Morelli L, Tompkins T.A. (2003). Sporeformers as human probiotics: Bacillus, Sporolactobacillus and Brevibacillus. Compr Rev Food Sci, F 2:101-110, doi: 10.1111/j.1541-4337.2003.tb00017.x.
  • Sarao, L.K., Arora, M. (2017). Probiotics, prebiotics, and microencapsulation: A review. Crit Rev Food Sci Nutr 57(2):344-371, doi: 10.1080/10408398.2014.887055.
  • Seyedain-Ardabili, M., Sharifan, A., Tarzi, B.G. (2016). The production of synbiotic bread by microencapsulation. Food Technol Biotechnol 54(1):52-59, doi: 10.17113/ftb.54.01.16.4234.
  • Shori, A.B. (2017). Microencapsulation improved probiotics survival during gastric transit. HAYATI J Biosci 24:1-5, doi: 10.1016/j.hjb.2016.12.008.
  • Siró, I., Kápolna, E., Kápolna, B., Lugasi, A. (2008). Functional food. Product development, marketing and consumer acceptance—A review. Appetite 51:456-467, doi: 10.1016/j.appet.2008.05.060.
  • Song, D., Ibrahim, S., Hayek, S. (2012). Recent application of probiotics in food and agricultural science. In: Probiotics, Rigobelo, E.C. (ed.), InTechOpen, doi: 10.5772/50121.
  • Soukoulis, C., Behboudi-Jobbehdar, S., Yonekura, L., Parmenter, C., Fisk, I.D. (2014a). Stability of Lactobacillus rhamnosus GG in prebiotic edible films. Food Chem 159:302-308, doi: 10.1016/j.foodchem.2014.03.008.
  • Soukoulis, C., Singh, P., Macnaughtan, W., Parmenter, C., Fisk, I.D. (2016). Compositional and physicochemical factors governing the viability of Lactobacillus rhamnosus GG embedded in starch-protein based edible films. Food Hydrocoll 52:876-887, doi: 10.1016/j.foodhyd.2015.08.025.
  • Soukoulis, C., Yonekura, L., Gan, H.H., Behboudi-Jobbehdar, S., Parmenter, C., Fisk, I. (2014b). Probiotic edible films as a new strategy for developing functional bakery products: The case of pan bread. Food Hydrocol 39:231-242, doi: 10.1016/j.foodhyd.2014.01.023.
  • Soyuçok, A., Başyiğit Kılıç, G. (2017). Süt kaynaklı olmayan probiyotik gıdalar. Türk Tarım-Gıda Bilim ve Teknoloji Dergisi 5(12):1615-1625, doi: 10.24925/turjaf.v5i12.1615-1625.1449.
  • Tanner, G., Matthews, K., Roeder, H., Konopasek, M., Bussard, A., Gregory, T. (2018). Current and future uses of probiotics. JAAPA 31(5):29-33, doi: 10.1097/01.JAA.0000532117.21250.0f.
  • Tripathi, M.K., Giri, S.K. (2014). Probiotic functional foods: survival of probiotics during processing and storage. J Funct Foods 9:225-241, doi: 10.1016/j.jff.2014.04.030.
  • Yonekura, L., Sun, H., Soukoulis, C., Fisk, I. (2014). Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: Technological characterization, storage stability and survival after in vitro digestion. J Funct Foods 6:205-214, doi: 10.1016/j.jff.2013.10.008.
  • Zanjani, M.A.K., Tarzi, B.G., Sharifan, A., Mohammadi, N., Bakhoda, H., Madanipour, M.M. (2012). Microencapsulation of Lactobacillus casei with calcium alginate-resistant starch and evaluation of survival and sensory properties in cream-filled cake. Afr J Microbiol Res 6(26):5511-5517, doi: 10.5897/AJMR12.972.
  • Zhang, L., Chen, X.D., Boom, R.M., Schutyser, M.A.I. (2018a). Survival of encapsulated Lactobacillus plantarum during isothermal heating and bread baking. LWT-Food Sci Technol 93:396-404, doi: 10.1016/j.lwt.2018.03.067.
  • Zhang, L., Huang, S., Ananingsih, V.K., Zhou, W., Chen, X.D. (2014). A study on Bifidobacterium lactis Bb12 viability in bread during baking. J Food Eng 122:33-37, doi: 10.1016/j.jfoodeng.2013.08.029.
  • Zhang, L., Taal, M., Boom, R.M., Chen, X.D., Schutyser, M.A.I. (2015). Viability of Lactobacillus plantarum P8 in bread during baking and storage. The 20th International Drying Symposium (IDS 2016), 07-10 August 2015, Gifu, Japan.
  • Zhang, L., Taal, M.A., Boom, R.M., Chen, X.D., Schutyser, M.A.I. (2018b). Effect of baking conditions and storage on the viability of Lactobacillus plantarum supplemented to bread. LWT-Food Sci Technol 87:318-325, doi: 10.1016/j.lwt.2017.09.005.
  • Zhang, N., Ju, Z., Zuo, T. (2018c). Time for food: The impact of diet on gut microbiota and human health. Nutrition 51-52:80-85, doi: 10.1016/j.nut.2017.12.005.