Emel ÜNAL TURHAN, Zerrin ERGİNKAYA, Ezgi SARIKODAL, Serya Tülin ÖZKÜTÜK, Gözde KONURAY

PROBİYOTİK BİTTER ÇİKOLATA ÜRETİMİNDE MİKROENKAPSÜLE LACTOBACILLUS RHAMNOSUS KULLANIMI

Bu çalışmanın amacı, probiyotik bitter çikolata üretiminde mikroenkapsüle Lactobacillus rhamnosus kullanımının farklı sıcaklıklardaki depolama süresince canlı probiyotik hücre sayısı ve duyusal özellikler üzerindeki etkisini araştırmaktır. Çalışmada probiyotik çikolata üretiminde kullanılan L. rhamnosus ekstrüzyon tekniği ile mikroenkapsüle edilmiş ve probiyotik çikolatalar 2 farklı sıcaklıkta (4oC ve 25oC) 60 gün boyunca depolanmıştır. Mikroenkapsülasyon işlemi çikolatanın erime sıcaklıklarında probiyotik kültürlere dayanım kazandırmıştır. 4oC’de depolamanın canlı hücre sayısını koruma yönünden daha elverişli olduğu tespit edilirken, 25oC’de depolama sonucunda çikolatalardaki probiyotik hücre sayısında ciddi düzeyde düşüş gözlenmiştir. Sonuç olarak bitter çikolata üretiminde mikroenkapsülasyon işlemi ve 4oC’de buzdolabı koşullarında depolama probiyotik hücre dayanımını arttırdığı için önerilmiştir.

Anahtar Kelimeler:

Çikolata, probiyotik, mikronkapslülasyon, L.rhamnosus

THE USE OF MICROENCAPSULATED LACTOBACILLUS RHAMNOSUS IN PROBIOTIC BITTER CHOCOLATE PRODUCTION

The aim of this study was to investigate the effects of the use of microencapsulated Lactobacillus rhamnosus in the production of probiotic bitter chocolate on probiotic viable cell count and sensory properties during storage at different temperatures. L. rhamnosus used in the production of probiotic chocolate was microencapsulated by extrusion technique and probiotic chocolate was stored at two different temperature (4oC and 25oC) throughout 60 days. The microencapsulation provided resistance to probiotic cultures at chocolate melting temperatures. While storage at 4oC was found more convenient to protect the survival of probiotic cells, storage at 25oC caused a serious decline in probiotic viability of tested microorganism in chocolates. As a result, microencapsulation technique and storage under refrigerator conditions (at 4oC) has been suggested to ensure the stability of probiotic cells in bitter chocolate production.

Keywords:

Chocolate, probiotic, microencapsulation, L. rhamnosus,

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