ORTAGONAL MERKEZİ KOMPOZİT TASARIM VE YÜZEY CEVAP METODOLOJİSİNDEN FAYDALANARAK BALIK YAĞI GIDA TAKVİYELERİNİN YAĞ ASİDİ KOMPOZİSYONLARININ GAZ KROMATOGRAFİSİ İLE TAYİNİ İÇİN YENİ BİR ESTERLEŞTİRME METODUNUN GELİŞTİRİLMESİ

Bu çalışmada kemometrik tekniklerden faydalanılarak MO'ların yağ asidi kompozisyonlarının tayini için etkili, uygulanabilir ve özel bir esterleştirme yöntemi geliştirilmiştir. Esterifikasyon parametrelerinden esterleşme sıcaklığı (A), esterleşme süresi (B) ve hekzan miktarı (C) ortagonal merkezi kompozit tasarım (OCCD) kullanılarak optimize edilmişlerdir. İlgili parametrelerin birbirleriyle ve cevap değeri (R) ile ilişkileri cevap yüzey metodolojisinden (RSM) faydalanılarak yorumlanmıştır. İlgili parametrelerin tespit edilen optimum değerleri esterleşme sıcaklığı için 60°C, esterleşme süresi için 40 dakika ve hekzan miktarı için 4.3 mL olarak tespit edilmiştir. Gaz kromatografisi ve geliştirilen yeni metot ile esterleştirilmesi kolay olmayan MO numuneleri kolaylıkla esterleştirilebilmiş ve başta eikosapentanoik asit (20:5) ve dokosahekzaenoik asit (22:6) olmak üzere tüm önemli yağ asitleri analiz edilebilmiştir

Anahtar Kelimeler:

Kemometri, esterleştirme, yağ asidi kompozisyonu, gaz kromatografisi, balık yağı gıda takviyesi

DEVELOPING A NEW ESTERIFICATION METHOD FOR THE DETERMINATION OF FATTY ACID COMPOSITIONS OF MARINE OIL SUPPLEMENTS BY GAS CHROMATOGRAPHY WITH ORTHOGONAL CENTRAL COMPOSITE DESIGN AND RESPONSE SURFACE METHODOLOGY

In this study, an efficient, applicable, and special esterification method for the quantification of fatty acid compositions of MOs has been created with chemometric techniques. The esterification variables which are the temperature of esterification (A), the time of esterification (B), and the amount of hexane (C), were optimized by using the orthogonal central composite design (OCCD). The relationships of the variables with each other and with the response value (R) are interpreted with response surface methodology (RSM). The optimal conditions were as follows: the temperature of esterification, 60°C; the time of esterification, 40 min; and the amount of hexane, 4.3 mL. Even MOs that are difficult to esterify can be easily esterified and the fatty acid compositions of all MOs, especially important fatty acids such as eicosapentaenoic acid (20:5) and docosahexaenoic acid (22:6), have been successfully analyzed by gas chromatography with the new method developed.

Keywords:

Chemometrics, esterification, fatty acid composition, gas chromatography, marine oil supplements,

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