FINDIĞIN POLİFENOLİK MADDELERİNİN ADSORBAN ÖZELLİKTE KOLON DOLGU MADDESİ KULLANILARAK FRAKSİYONLARINA AYRILMASI VE ELDE EDİLEN FRAKSİYONLARIN KARAKTERİZASYONU

Bu çalışmanın amacı, fındığın içerdiği polifenolik maddelerin fraksiyonlarKına ayrılması ve elde edilen fraksiyonların karakterizasyonlarının gerçekleştirilmesidir. Bu amaçla, fındığın fenolik maddeleri %80 (v/v) asetonla ekstrakte edilerek kolona verilmiştir. Düşük molekül ağırlıklı (LMW) polifenolik maddeler etanol, yüksek molekül ağırlıklı (HMW) polifenolik maddeler ise aseton kullanılarak fraksiyonlanmıştır. Ayrıca LMW fraksiyonu, UV’de (280 nm’de) verdiği pik noktalarına göre dört ayrı fraksiyona ayrılmıştır. Sonuçlar değerlendirildiğinde, tüm analizlerde en yüksek değerlere sahip olan Fraksiyon V’in toplam fenolik madde ve kondense tanen miktarları sırasıyla, 77.9 mg gallik asit eşdeğer (GAE)/g ekstre ve 227 mg kateşin eşdeğer (KE)/ g ekstre; antioksidan kapasite değerleri DPPH, ABTS ve FRAP yöntemleri için sırasıyla 0.047 mg/mL IC50 değeri, 1.442 mmol Troloks Eşdeğer (TE)/ g ekstre, 307 mg FeSO4.7H2O/g ekstre olarak tespit edilmiştir. Çalışma sonucunda, LMW fraksiyonlarının ayrı fraksiyonlar olarak toplanmasına gerek olmadığı, HMW fraksiyonundan ayrılmasının yeterli olduğu tespit edilmiştir. HMW fraksiyonu, yüksek tanen içeriğiyle polifenolik maddeler açısından önemli bir kaynak olarak değerlendirilebilir.

Anahtar Kelimeler:

Fraksiyonlama, fındık, antioksidan aktivite, kondense taninler, toplam fenolik madde

FRACTIONATION OF HAZELNUT POLYPHENOLIC COMPOUNDS BY USING ADSORBENT FEATURED COLUMN PACKING MATERIAL AND CHARACTERIZATION OF OBTAINED FRACTIONS

The aim of this study is fractionation of hazelnut polyphenols and their characterization. For this aim, phenolic compounds were extracted with 80 % (v/v) acetone and loaded to column. Low molecular weight (LMW) and high molecular weight fractions (HMW) were fractionated with ethanol and acetone, respectively. Furthermore, LMW fraction was fractionated into four parts according to peaks observed at UV (280nm). FractionV had the highest contents/activities; total phenolics and condensed tannins contents were 77.9 mg gallic acid equivalent (GAE)/g extract and 227 mg catechin equivalent (CE)/ g extract, respectively. Antioxidant activities of FractionV were 0.047 mg/mL IC50 value, 1.442 mmol Trolox equivalent (TE)/g extract, 307 mg FeSO4.7H2O/g extract for DPPH, ABTS and FRAP assays, respectively. As a result, it is seen that further fractionation of LMW is not needed and separation of LMW from HMW fraction is enough. HMW fraction could be utilized as a potential source of polyphenols.

Keywords:

Antioxidant activity, hazelnut, fractionation, condensed tannins, total phenolics content,

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Alasalvar C., Bolling B.W. (2015). Review of nut phytochemicals, fat-soluble bioactives, antioxidant components and health effects. Brit J Nutr, 113: 68-78, doi: 10.1017/S0007114514003729.
Alasalvar C., Karamac´ M., Amarowicz R., Shahidi F. (2006). Antioxidant and antiradical activities in extracts of hazelnut kernel (Corylus avellana L.) and hazelnut green leafy cover. J Agric Food Chem, 54: 4826-4832, doi: https://doi.org/10.1021/jf0601259.
Alasalvar C., Karamac´, M., Kosińska A., Rybarczyk A., Shahidi F., Amarowicz, R. (2009). Antioxidant activity of hazelnut skin phenolics. J Agric Food Chem, 57: 4645-4650, doi: https://doi.org/10.1021/jf900489d.
Alaşalvar C., Pelvan E. (2009). Günümüzün ve geleceğin gıdaları fonksiyonel gıdalar. Bilim ve Teknik, 501: 26-29.
Amarowicz R., Estrella I., Hernández T., Dueñas M., Troszyńska, A., Kosińska A., et al. (2009). Antioxidant activity of a red lentil extract and its fractions. Int J Mol Sci, 10: 5513-5527, doi: 10.3390/ijms10125513.
Amarowicz R., Troszyńska A., Shahidi F. (2005). Antioxidant activity of almond seed extract and its fractions. J Food Lipids, 12: 344-358, doi: https://doi.org/10.1111/j.1745-4522.2005.00029.x.
Arapitsas P. (2012). Hydrolyzable tannin analysis in food. Food Chem, 135: 1708-1717, doi: https://doi.org/10.1016/j.foodchem.2012.05.096. Armada P.L., Rivas S., Gonzalez B., Moure A. (2019). Extraction of phenolic compounds from hazelnut shells by green processes. J Food Eng, 255: 1-8, doi: https://doi.org/10.1016/j.jfoodeng.2019.03.008.
Barreira J.C.M., Ferreira I.C.F.R., Oliveira M.B.P.P., Pereira J.A. (2008). Antioxidant activities of the extracts from chestnut flower, leaf, skins and fruit. Food Chem, 107: 1106–1113, doi: https://doi.org/10.1016/j.foodchem.2007.09.030.
Benzie I.F.F., Strain J.J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol, 299: 15-27, doi: 10.1016/s0076-6879(99)99005-5.
Contini M, Baccelloni S, Massantini R, Anelli G. (2008). Extraction of natural antioxidants from hazelnuts (Corylus avellana L.) shell and skin wastes by long maceration at room temperature. Food Chem, 110: 659-669, doi: https://doi.org/10.1016/j.foodchem.2008.02.060.
de la Rosa L.A., Alvarez-Parrilla E, Shahidi F. (2011). Phenolic compounds and antioxidant activity of kernels and shells of Mexican pecan (Carya illinoinensis). J Agric Food Chem, 59: 152-62, doi: 10.1021/jf1034306.
Delgado T., Malheiro R., Pereira J.A., Ramalhosa, E. (2010). Hazelnut (Corylus avellana L.) kernels as a source of antioxidants and their potential in relation to other nuts. Ind Crops Prod, 32: 621–626, doi: https://doi.org/10.1016/j.indcrop.2010.07.019.
Ghirardello D., Bertolino M., Belviso S., Belloa B.D., Giordano M., Rolle L., Gerbi V., Antonucci M., Spigolon N., Zeppa G. (2016). Phenolic composition, antioxidant capacity and hexanal content of hazelnuts (Corylus avellana L.) as affected by different storage conditions. Postharvest Biol Tech, 112: 95-104, doi: https://doi.org/10.1016/j.postharvbio.2015.09.039.
Gini T.G., Jothi G.J. (2018). Column chromatography and HPLC analysis of phenolic compounds in the fractions of Salvinia molesta Mitchell. Egypt J Basic Appl Sci, 5: 197–203, doi: https://doi.org/10.1016/j.ejbas.2018.05.010.
Gu L, Kelm M.A., Hammerstone J.F., Beecher G., Holden J., Haytowitz D., Gebhardt S., Prior R.L. (2004). Concentrations of proanthocyanidins in common foods and estimations of normal consumption. J Nutr, 134: 613−617, doi: 10.1093/jn/134.3.613.
International Nut & Dried Fruit Council Foundation (INC), International Nut & Dried Fruits Statistical Year Book 2018/2019, https://www.nutfruit.org/files/tech/1553521370_INC_Statistical_Yearbook_2018.pdf. (Erişim tarihi: 01 Temmuz 2019).
Kamiloglu S., Pasli A.A., Ozcelik B., Capanoglu E. (2014). Evaluating the in vitro bioaccessibility of phenolics and antioxidant activity during consumption of dried fruits with nuts. LWT - Food Sci Technol, 56: 284-289, doi: https://doi.org/10.1016/j.lwt.2013.11.040.
Karamać M., Kosińska A., Rybarczyk A., Amarowicz R. (2007). Extraction and chromatographic separation of tannin fractions from tannin-rich plant material. Pol J Food Nutr Sci, 57: 471-474.
Koroleva O., Torkova A., Nikolaev I., Khrameeva E., Fedorova T., Tsentalovich M., et al. (2014). Evaluation of the antiradical properties of phenolic acids. Int J Mol Sci, 15: 16351-16380, doi: 10.3390/ijms150916351.
Lainas K., Alasalvar C., Bolling B.W. (2016). Effects of roasting on proanthocyanidin contents of Turkish Tombul hazelnut and its skin. J Funct Foods, 23: 647-653, doi: https://doi.org/10.1016/j.jff.2016.03.029.
Locatelli M., Travaglia F., Coïsson J.D., Martelli A., Stévigny C., Arlorio M. (2010). Total antioxidant activity of hazelnut skin (Nocciola Piemonte PGI): Impact of different roasting conditions. Food Chem, 119: 1647-1655, doi: https://doi.org/10.1016/j.foodchem.2009.08.048.
Milevskaya V.V., Prasad S., Temerdashev Z.A. (2019). Extraction and chromatographic determination of phenolic compounds from medicinal herbs in the Lamiaceae and Hypericaceae families: A review. Microchem J, 145: 1036–1049, doi: https://doi.org/10.1016/j.microc.2018.11.041.
Monagas M., Garrido I., Lebron-Aguilar R., Gomez-Cordoves M.C., Rybarczyk A., Amarowicz R., et al. (2009). Comparative flavan-3-ol profile and antioxidant capacity of roasted peanut, hazelnut, and almond skins. J Agric Food Chem, 57: 10590-10599, doi: 10.1021/jf901391a.
Mueller-Harvey I. (2001). Analysis of hydrolysable tannins. Anim Feed Sci Technol, 91: 3-20, doi: https://doi.org/10.1016/S0377-8401(01)00227-9.
Naczk M., Shahidi F. (2004). Extraction and Analysis of Phenolics in Food. J Chromatogr, 1054: 95-111, doi: https://doi.org/10.1016/j.chroma.2004.08.059.
Napolitano A., Cerulli A., Pizza C., Piacente S. (2018). Multi-class polar lipid profiling in fresh and roasted hazelnut (Corylus avellana cultivar “Tonda di Giffoni”) by LC-ESI/LTQOrbitrap/MS/MSn. Food Chem, 269: 125-135, doi: 10.1016/j.foodchem.2018.06.121.
Pelvan E., Alasalvar C., Uzman S. (2012). Effects of roasting on the antioxidant status and phenolic profiles of commercial Turkish hazelnut varieties (Corylus avellana L). J Agric Food Chem, 60: 1218-1223, doi: 10.1021/jf204893x.
Pelvan Pelitli E., Janiak M.A., Amarowicz R., Alasalvar C. (2017). Protein precipitating capacity and antioxidant activity of Turkish Tombul hazelnut phenolic extract and its fractions. Food Chem, 218: 584-590, doi: https://doi.org/10.1016/j.foodchem.2016.09.070.
Price M.L., Scoyoc S.V., Butler L.G. (1978). A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem, 26: 1214-1218, doi: https://doi.org/10.1021/jf60219a031.
Shahidi F, Alasalvar C, Liyana-Pathirana C.M. (2007). Antioxidant phytochemicals in hazelnut kernel (Corylus avellana L.) and hazelnut byproducts. J Agric Food Chem, 55: 1212-1220, doi: https://doi.org/10.1021/jf062472o.
Taş N.G., Gökmen V. (2015). Bioactive compounds in different hazelnut varieties and their skins. J Food Comp Anal, 43: 203-208, doi: https://doi.org/10.1016/j.jfca.2015.07.003.
Taş N.G., Yılmaz C., Gökmen V. (2019). Investigation of serotonin, free and protein-bound tryptophan in Turkish hazelnut varieties and effect of roasting on serotonin content. Food Res Int, 120: 865-871, doi: https://doi.org/10.1016/j.foodres.2018.11.051.
United States Department of Agriculture (USDA). Database for the Proanthocyanidin Content of Selected Foods, http://www.nal.usda.gov/fnic/foodcomp. (Erişim tarihi: 15 Aralık 2011).
Yuan B., Lu M., Eskridge K.M., Isom L.D., Hanna M.A. (2018). Extraction, identification, and quantification of antioxidant phenolics from hazelnut (Corylus avellana L.) shells. Food Chem, 244: 7-15, doi: https://doi.org/10.1016/j.foodchem.2017.09.116.