FERMENTE GIDALARDAN İZOLE EDİLEN LAKTİK ASİT BAKTERİLERİNİN ANTİFUNGAL AKTİVİTESİNİN BELİRLENMESİ

Bu çalışmada fermente ürünlerden izole edilen 45 laktik asit bakterisinin antifungal etkileri araştırılmıştır. Laktik asit bakterileri, mikotoksigenik Aspergillus flavus, Aspergillus fumigatus, Aspergillus parasiticus, Cladosporium cladosporioides, Gibberella accuminata, Gibberella intricans, Fusarium oxysporum, Fusarium incarnatum, Penicillium chrysogenum, Rhizopus stolonifer'e karşı çift tabakalı kültür yöntemi ve kuyu yöntemi kullanılarak antifungal aktivite açısından taranmıştır. Dokuz laktik asit bakteri izolatı çift tabakalı agar yöntemi ile antifungal aktivite gösterirken, on dokuz laktik asit bakterisinin süpernatantı bir veya daha fazla küfe karşı antifungal aktivite göstermiştir. Yüksek etkili olarak belirlenen L.brevis KL7'nin süt tozu ortamında A. parasiticus ve P. chrysogenum'a olan etkisi araştırılmış ve 120 saatte her iki küfü inhibe ettiği saptanmıştır. Gıda üretiminde ve gıdaların raf ömrünün uzatılmasında L. brevis KL7 ümit vaat eden bir izolat olarak belirlenmiştir.

Anahtar Kelimeler:

Laktik asit bakterileri, küf, antifungal aktivite, süpernatant

SCREENING OF ANTIFUNGAL ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM FERMENTED FOODS

In this study, we investigated the antifungal effects of 45 lactic acid bacteria (LAB) that were isolated from fermented products. Lactic acid bacteria were screened for antifungal activity by using dual agar overlay and well method against mycotoxigenic Aspergillus flavus, Aspergillus fumigatus, Aspergillus parasiticus, Cladosporium cladosporioides, Gibberella accuminata, Gibberella intricans, Fusarium oxysporum, Fusarium incarnatum, Penicillium chrysogenum, Rhizopuss stolonifer. Nine LAB isolates showed antifungal activity with dual agar overlay method. Cell free supernatant of 19 LAB isolates showed antifungal activity against one or more fungi species. The effects of L. brevis KL7, which was determined as high effective, against A. parasiticus and P. chrysogenum in the milk medium were investigated; and it was found that both fungi were inhibited by L. brevis KL7 at 120 hours. L. brevis KL7 can be a promising isolate for food production and extention of the shelf life of the food.

Keywords:

Lactic acid bacteria, mold, antifungal activity, supernatant,

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