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BROİLER ETLERİNDEKİ TETRASİKLİN VE FLORFENİKOL ANTİBİYOTİK KALINTILARININ ELISA İLE GÖRÜNTÜLENMESİ VE SIVI KROMATOGRAFİSİ KÜTLE/ KÜTLE SPEKTROMETRESİ İLE DOĞRULANMASI

Bu çalışmada Aydın ilinde tüketime sunulan broiler etlerindeki tetrasiklin ve florfenikol antibiyotik kalıntılarının araştırılması amaçlanmıştır. Bu amaçla farklı satış yerlerinden farklı zamanlarda toplanan 80 adet broiler örneği kullanılmıştır. Antibiyotik kalıntı varlığı ELISA test kitleriyle belirlenmiş ve LC-MS/MS cihazı ile doğrulama yapılmıştır. ELISA test sonuçlarına göre incelenen örneklerin 24’ünün (%30) tetrasiklin pozitif olduğu ve örneklerin hiçbirinde florfenikole rastlanılmadığı gözlenmiştir. Tetrasiklin içeren ve LC-MS/MS’de doğrulaması yapılan 24 örnekteki antibiyotik düzeyi ortalama 30 ppm olarak bulunmuştur. Bulunan değerin maksimum kalıntı düzeyinin (MRL) altıda olduğu görülmüştür.

Anahtar Kelimeler:

tetrasiklin, ELISA, Florfenikol, LC-MS/MS

SCREENING OF TETRACYCLINE AND FLORFENICOL ANTIBIOTIC RESIDUES IN BROILER MEAT USING ELISA AND CONFIRMATION BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY

The aim of this study was to investigate the presence of tetracycline and florfenicol residues in broiler meat that sailed in Aydın city, Turkey. For this purpose, 80 broiler meats that brands of local and national commercial were used. ELISA technique was used to investigate the presence of antibiotic residues and liquid chromatography/ tandem mass spectrometry (LC-MS/MS) technique was used to confirm the residue. As result of the ELISA, 24 samples 30%) were positive for tetracycline. Florfenicol residues were found in any of the samples. 24 samples that is confirmed by LC-MS/MS were included an average of 30 ppb level. Residual amounts of the samples were found to be below the maximum residue limit (MRL) values.

Keywords:

Florfenicol, tetracycline, ELISA, LC-MS/MS,

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1. Aarestrup, F. (2012). Sustainable farming: Get pigs off antibiotics. Nature, 486: 465–466. 2. Cháfer-Pericás, C., Maquieira, A., Puchades, R. (2010). Fast screening methods to detect antibiotic residues in food samples TrAC Trends in Analytical Chemistry 29: 9,1038-1049. 3. Passantino, A. and Russo, C. (2008). Maximum Residue Levels of Veterinary Medicines in Relation to Food Safety: European Community Legislation and Ethical Aspects J. Verbr. Lebensm, 3: 351 – 358. 4. Gomes, E. R. and Demoly, P. (2005). Epidemiology of hypersensitivity drug reactions. Curr Opin Allerg Clin Immunol, 5: 309 – 316. 5. Martínez, U. Z. (2005). Health official: clenbuterol cases rising. Miami Herald, Mexico Edition, 28 November 2005. Available at: http:// www.eluniversal.com.mx/miami/15989.html 6. Raison-Peyron, N., Messaad, D., Bousquet, J., and Demoly, P. (2001). Anaphylaxis to beef in penicillin-allergic patient. Allergy, 56: 796 – 797. 7. Carrique-Mas, J.,J., Trung, N., V., Hoa, N.,T., Mai, H.,H., Thanh, T.,H., James. Campbell, J., A., et al. (2015). Antimicrobial Usage in Chicken Production in the Mekong Delta of Vietnam. Zoonoses and Public Health, 1: 70-78. 8. European Commission (2002). Commission Decision 2002/657/EC of 14 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and interpretation of results. Off J L, 221: 8-36. 9. Acet, A., Ates, M., Erganis, O. (1998). Determination of antimicrobial residues in animal tissues by agar diffusion technique. Journal of Selcuk University Faculty of Veterinary Medicine, 3: 197-205. 10. Akar F. (1991). Investigating some antibiotic residues with thin layer chromatography methods in chicken meat and liver that was sold in Ankara. The Scientific and Technical Research Council of Turkey. 11. Obekci J. (2002). Determination of the tetracycline group of antibiotics in chicken meat and liver by HPLC. Ministry of Agriculture and Rural Affairs. 12. Bergner-Lang, B., Bourgeois, B., Edelhauser, M., Klein, E., Lippold, R., Mollers, M., Pletscher, D. (1993). Chemical Drug Residue Analysis of Inhibitor-positive Samples of Meat, Kidney and Liver. Editors: Haagsma N, Ruıter A, Eysenberg PBC. Euro Residue II, Conferance of Residues of Veterinary Drugs in Food. Veldhoven, The Netherlands, 186-191 p. 13. Onal, A., Aydın, N., Ayaz, Y., Iscan, D., Savas, N. (1993). To be detected some antibiotics present in milk and meat by various methods. Journal of Veterinary Microbiology Institute, 7: 48-51. 14. Lee, J.B., Chung, H..H, Chung, Y.H., Lee, K.G. (2005). Development of an analytical protocol for detecting antibiotic residues in various foods. Food Chem., 105: 1726-1731.