Suna ÖZDEMİR, Osman BALCI, Halime GÖKTEPE, Hüseyin GÖRKEMLİ, Elmas TAŞÇI, Hasan ACAR

Tekrarlayan gebelik kaybı olan hastalarda trombofili mutasyon sıklığının değerlendirilmesi

Amaç: Çalışmada daha önce en az üç tane gebelik kaybı olan hastalarda bazı trombofilik faktörlerin sıklığının sağlıklı kontrol grubu ile karşılaştırılarak değerlendirilmesi amaçlandı. Yöntem: Çalışmaya 2005-2009 yılları arasında kliniğimize başvuran toplam 301 hasta alındı. Bu hastalar iki gruba ayrıldı. Grup 1 daha önce üç veya daha fazla 20 haftanın altında gebelik kaybı olan 251 hastadan oluştu. Grup 2 aha önce en az 1 tane sorunsuz gebeliği olan ve hiç düşük yapmamış 50 kadın hastayı kapsadı. Tüm kadınlar Faktör V Leiden (FVL), protrombin (G20210A) ve metilentetrahidrofolat redüktaz (MTHFR) C677T varlığı açısından PCR-RFLP yöntemi ile test edildi. Veriler istatiksel açıdan ki-kare testi, student-t ve Mann-Whitney testleri kullanılarak analiz edildi. Bulgular: Her iki grubun yaş ortalaması benzer olarak saptandı. Her üç trombofilik faktör heterozigot ve homozigot olarak incelendi. Değerlendirme sonucunda her üç mutasyon FVL, protrombin, MTHFR bölgesi için de çalışma ve kontrol arasında anlamlı olarak fark tespit edilemedi (p>0.05). Çalışma grubunda 134 (% 53.3) hastada, kontrol grubunda ise 23 (% 46) hastada en az bir trombofilik faktör saptandı. Protrombin homozigot mutasyon her iki grubta da bulunamadı. FVL homozigot mutasyon kontrol grubundaki hiçbir hastada tespit edilmedi. Sonuç: Çalışmadan elde edilen veriler FVL, protrombin ve MTHFR mutasyonları ile tekrarlayan gebelik kayıpları arasında anlamlı bir ilişki olmadığını desteklemektedir.

Evaluation of the frequency of thrombophilic mutations in patients with recurrent pregnancy loss

Objective: The present study was undertaken to evaluate the frequency of some thrombophilic factors in patients with recurrent pregnancy loss (RPL) by comparing with healthy controls. Methods: A total of 301 patients admitted to our clinic between 2005-2009 was included in this study. These patients were divided into two groups. Group 1 consisted of 251 patients with at least three pregnancy loss < 20 week. Group 2 included 50 women with at least one uneventful pregnancy and no previous history of miscarriage. All women were tested for Factor V Leiden (FVL), prothrombin (G20210A), and methylenetetrahydrofolate reductase (MTHFR) C677T mutations. Data were analyzed using chi-square and student-t tests. Results: The mean age in each group was similar. Thrombophilic factors were investigated as heterozygote and homozygote mutations. Similar prevalences of FVL, prothrombin and MTHFR mutations were found in both groups (p>0.05). At least one inherited thrombophilic factor was detected in 134 (53.3%) women in the study group, and 23 (46%) women in controls. Prothrombin homozygote mutation was not determined in both groups. FVL homozygote mutation was not found in controls. Conclusion: The results of the present study suggest that there is no significant relation between RPL and the mutations of FVL, prothrombin and MTHFR.

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