OĞUZ ÖZÇELİK, SERMİN ALGÜL, Mustafa DENİZ, Fatma BAYDAŞ, Fatih TAN

Sabah ve gece yapılan aerobik egzersizin antrenmanlı deneklerde serum irisin düzeyine etkilerinin belirlenmesi

Amaç: Egzersizin enerji homeostazisinin düzenlenmesinde önemli yararlı etkileri olduğu bilinmektedir. Enerji düzenlenmesi ile ilgili hormon düzeylerinin değişiminde egzersizin etkisi araştırmacılar arasında yoğun bir ilgiye sahip olan ilginç bir konudur ve irisin bu konuda özel bir öneme sahiptir. Bu çalışmada antrenmanlılarda irisin düzeyine sabah ve gece yapılan akut egzersizin olası yararlı etkilerinin araştırılması amaçlanmıştır.Gereç ve Yöntem: Yirmi antrenmanlı erkek denek sabah ve geceaerobik egzersiz koşu egzersizine katılmışlardır. Egzersizden önce ve sonra venöz kan örnekleri alınmıştır. Serum irisin düzeyi ELISA yöntemiyle ölçülmüştür.Bulgular: Bazal irisin düzeyi her iki testte de istatistiksel olarak anlamlı değişmemiştir. Fakat irisin düzeyi sabah (262.01±37 ng/ml'den 299.74±39 ng/ml'ye) ve gece (268.57±30 ng/ml'den 316.04±33 ng/ml'ye) istatistiksel olarak anlamlı oranda artış göstermiştir(p < 0.0001). Sonuç: Bu çalışma sonuçları egzersiz antrenmanı zamanının antrenmanlı deneklerde irisin düzeyinde farklı etkilere sahip olmadığını göstermektedir.Bu deneysel düzenek egzersizin enerji düzenlenmesinde bir role sahip olan irisini artırdığını açıkça göstermektedir.

Objective: It is known that exercise has important beneficial effects on regulation of energy homeostasis. Effect of exercise on alteration of hormone levels engaged in energy regulation is an interesting topic that has obtained a lot of consideration among investigators, with a special attention on irisin. This study was aimed to investigate possible beneficial effect of acute exercise, performed at morning and at night on irisin levels in trained subjects. Materials and Methods: Twenty trained male subjects performed aerobic running exercise in morning and at night. Pre and post exercise venous blood samples were taken. Serum irisin levels were analysed using Enzyme Linked-Immunosorbent Assay. Results: Baseline irisin levels were not statistically significantly different in both tests. However, irisin levels were increased significantly in morning (from 262.01±37 ng/ml to 299.74±39 ng/ml) and night (from 268.57±30 ng/ml to 316.04±33 ng/ml) (p < 0.0001). Conclusion: This study documents that time of exercise workout has no different effects on irisin levels in trained subjects. This experimental setting is clearly showed that exercise increases in irisin levels that have roles in regulation of energy expenditure.

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1. Boström P, Wu J, Jedrychowski MP, et al. A PGC1 ? dependent myokine that drives Brown fat-like development of white fat and thermogenesis. Nature 2012;481:463-8.

2. Mahajan RD, Patra SK. Irisin, a novel myokine responsiblefor exercise induced browning ofwhite adipose tissue. Indian J ClinBiochem 2013;28:102-3.

3. Huh JY, Panagiotou G, Mougios V, et al. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism 2012;61:1725-38.

4. Zhu D, Wang H, Zhang J, et al. Irisin improves endothelial function in type 2 diabetes through reducing oxidative/nitrative stresses. J Mol Cell Cardiol 2015;87:138-47.

5. Tsuchiya Y, Ando D, Goto K, et al. High-intensity exercise causes greater irisin response compared with low-intensity exercise under similar energy consumption. Tohoku J Exp Med 2014;233:135-40.

6. Daskalopoulou SS, Cooke AB, Gomez YH, et al. Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. Eur J Endocrinol 2014;171:343-52.

7. Brenmoehl J, Albrecht E, Komolka K, et al. Irisin is elevated in skeletal muscle and serum of mice immediately after acute exercise. Int J Biol Sci 2014;10:338-49.

8. Kim HJ, So B, Choi M, Kang D, Song W. Resistance exercise training increases the expression of irisin concomitant with improvement of muscle function in aging mice and humans. ExpGerontol 2015;70:11-7.

9. Czarkowska-Paczek B, Zendzian-Piotrowska M, Gala K, Sobol M, Paczek L. One session of exercise or endurance training does not influence serum levels of irisin in rats. J Physiol Pharmacol 2014;65:449-54.

10. Pekkala S, Wiklund PK, Hulmi JJ, et al. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health? J Physiol 2013;591:5393- 5400.

11. Kurdiova T, Balaz M, Vician M, et al. Effects of obesity, diabetes and exercise on Fndc5 gene expressionand irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies. J Physiol 2014;592:1091-1107.

12. Hecksteden A, Wegmann M, Steffen A, et al. Irisin and exercise training in humans - resultsfrom a randomizedcontrolled training trial. BMC Med 2013;11:235.

13. Norheim F, Langleite TM, Hjorth M, et al. The effects of acute and chronic exercise on PGC-1alpha, irisin and browning of subcutaneous adipose tissue in humans. FEBS J 2014;281:739-49.

14. Qiu S, Cai X, Sun Z, et al. Chronic Exercise Training andCirculating Irisin in Adults: A Meta-Analysis. Sports Med 2015;45:1577-88.

15. Kaya H, Ozcelik O. Tıp Öğrencilerinde Bir Yılda Vücut Kompozisyonlarında Meydana Gelen Değişimlerin Belirlenmesi. Firat Med J 2005;10:164-8.

16. Karvonen JJ, Kentala E, Mustala O. The effects of training on heart rate: a "longitudinal" study. Ann Med Exp BiolFenn 1957;35:307-15.

17. American College of Sports Medicine (ACSM). Guidelines for ExerciseTestingand Prescription (9th ed.). Baltimore, MD: Lippincott, Williams andWilkins, 2014.

18. Ozcelik O, Ozkan Y, Algul S, Colak R. Beneficial effects of training at the anaerobic threshold in addition to pharmaco therapy on weight loss, body composition, and exercise performance in women with obesity.Patient Prefer Adherence 2015;9:999-1004.

19. Roca-Rivada A, Castelao C, Senin LL, et al. FNDC5/irisin is not only a myokine but also an adipokine. PLoS One 2013;8:60563.

20. Scalzo RL, Peltonen GL, Giordano GR, et al. Regulators of human white adipose browning: evidence for sympathetic control and sexual dimorphic responses to sprint interval training. PLoS One 2014;9:90696.

21. Swick AG, Orena S, O'Connor A. Irisin levels correlate with energy expenditure in a subgroup of humans with energy expenditure greater than predicted by fat free mass. Metabolism2013;62:1070-73.

22. Loeffler D, Mueller U, Scheuermann K, et al. Serum irisin levels are regulated by acute strenuous exercise. J ClinEndocrinolMetab 2015;100:1289-99.

23. Anastasilakis AD, Polyzos SA, Saridakis ZG, et al. Circulating irisin in healthy, young individuals: day-night rhythm, effects of food intake and exercise, and associations withgender, physical activity, diet, and body composition. J Clin Endocrinol Metab 2014;99:3247-355.

24. Tsuchiya Y, Ando D, Takamatsu K, Goto K. Resistance exercise induces a greater irisin response than endurance exercise. Metabolism 2015;64:1042-50.

25. Kurdiova T, Balaz M, Vician M, et al. Effects of obesity, diabetes and exercise on Fndc5 gene expression and irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies. J Physiol 2014;592:1091-107.

26. Irving BA, Still CD, Argyropoulos G. Does irisin have a brite future as a therapeuticagent in humans? Curr Obes Rep 2014;3:235-41.