MUSTAFA YAŞAR ÖZDAMAR, Müslim YURTÇU, Hatice TOY, MEHMET AKÖZ, ENGİN GÜNEL

Renal iskemi-reperfüzyon hasarında üzüm çekirdeği proantosiyanidin ekstresinin etkisi

Amaç: Bu çalışmada üzüm çekirdeği proanthocyanidin ekstresinin (GSPE) renal iskemi-reperfüzyon (I-R) hasarında profilaktik etkisini araştırmak amaçlandı. Yöntem: Kırk adet sıçan her biri 10 sıçandan oluşan 4 gruba ayrıldı: Kontrol (K), sham (S), iskemi-reperfüzyon (I-R) ve I-R+ GSPE. K grubunda her iki renal pedikül eksplore edildi. S grubunda sol renal pedikül eksplore edildi ve sağ nefrektomi yapıldı. I-R ve I-R+GSPE gruplarında sağ nefrektomi sonrası, sol renal pedikülde oklüzyon (45 dakika) ve reperfüzyon (60 dakika) gerçekleştirildi. Bununla birlikte, I-R+GSPE grubuna cerrahiden önce 15 gün süre ile 15 mg/kg GSPE verildi. Dokuda malondialdehid (MDA) ve glutatyon redüktaz (GR) seviyesi; serumda üre, kreatinin, aspartat aminotransferaz (AST) ve tümör nekroz faktor-α (TNF-α) değerlendirildi. Bulgular: I-R grubundaki MDA seviyeleri S ve I-R+GSPE gruplarındaki MDA seviyelerinden anlamlı olarak yüksekti. I-R grubundaki GR seviyeleri, S ve I-R+GSPE gruplarındaki GR seviyelerinden anlamlı olarak daha düşüktü. I-R grubundaki üre, kreatinin, AST ve TNF-α seviyeleri K, S ve IR+ GSPE gruplarındaki seviyelerden daha yüksekti. Sonuç: Profilaktik GSPE tedavisi renal I-R hasarını önemli oranda azaltır.

The effect of grape seed proanthocyanidin in renal ischemia-reperfusion injury

Objective: This study aimed to determine the prophylactic effect of grape seed proanthocyanidin extract (GSPE) in ischemia-reperfusion (I-R) injury-induced renal tissue damage. Methods: Forty rats were divided into 4 groups each containing 10 rats: control (C), sham (S), I-R, and I-R+GSPE. Both renal pedicles were explored in the C group and the left renal pedicle was explored and right nephrectomy was performed in the S group. Occlusion (45 minutes) and reperfusion (60 minutes) of the left renal pedicle were performed after right nephrectomy in the I-R and I-R+GSPE groups. However, 15 mg/kg GSPE was given to the I-R+GSPE group for 15 days before surgery. The levels of malondialdehyde (MDA) and glutathione reductase (GR) in tissue and urea, and levels of creatinine, aspartate aminotransferase (AST), and tumor necrosis factor-α (TNF-α) in serum were evaluated. Results: MDA levels in the I-R group were significantly higher than those in the S and I-R+GSPE groups. GR levels in the I-R group were significantly lower than those in the S and I-R+GSPE groups. Urea, creatinine, AST, and TNF-α levels in the I-R group were higher than those in C, S, and I-R+GSPE groups. Conclusion: Prophylactic GSPE treatment significantly decreases renal I-R injury.

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