Ahmet Kağan KARABULUT, İSMİHAN İLKNUR UYSAL, Ahmet Levent BAŞ, Nadire ÜNVER DOĞAN, ZELİHA FAZLIOĞULLARI, Hasan ACAR

Nerium oleander'in liyofilize sıvı distilatının rat embriyoları gelişimi üzerine toksik ve teratojen etkilerinin in vitro kültür ortamında araştırılması

Amaç: Nerium oleander, her mevsimde yeşil kalan bir çalı türüdür. Halk arasında antidiabetik, antilipidemik ve geniş spektrumlu bir ilaç olarak kullanılır. Bu çalışmada Nerium oleander sıvı distilatının kültür ortamında gelişen rat embriyolarına etkilerinin araştırılması planlandı. Gereç ve yöntem: 9,5 günlük rat embriyoları, değişik konsantrasyonlarda (0.2 - 8 mg/ml) Nerium oleander ilave edilen rat serumunda 48 saat kültüre edildi. Her bir konsantrasyon için en az 10 embriyo kullanıldı. İlacın sıvı distilatı hidrodistilasyon metodu ile elde edildi. Nerium oleander'in toplam morfolojik skor, yolk sak çapı, tepe-kıç mesafesi ve somit sayısı gibi embriyonik gelişimsel parametreler üzerine doz bağımlı etkileri morfolojik metod ile karşılaştırıldı. Embriyolar malformasyonlar bakımından da değerlendirildi. İlacın genotoksisitesi DNA fragmentasyon metodu ile değerlendirildi. Bulgular: Değişik konsantrasyonlardaki Nerium oleander ilavesi ile kültüre edilen embriyolar kontrol grubu ile karşılaştırıldığında ilacın herhangi bir büyüme geriliği ya da malformasyona sebep olmadığı gözlendi. Sonuç: Bu sonuçlar Nerium oleander'in gebelikte, gelişen embriyo üzerine negatif etkisi olmadığını göstermiştir. Ancak bu veriler ileri in vivo deneyler ve hücre hattı çalışmaları ile desteklenmelidir.

Investigation of developmental toxicity and teratogenicity of lyophylised aqueous distillate of Nerium oleander on rat embryos cultured in vitro

Objectives: Nerium oleander, an evergreen shrub, is used in folklore medicine as an antidiabetic and antilipidemic and exhibits a wide spectrum of bioactivities. This study was planned to investigate the effects of Nerium osleander lyophilised distillate on developing rat embryos in culture. Materials and methods: Postimplantation rat embryos of day 9.5 were cultured for 48 h in whole rat serum with the addition of various concentrations of Nerium oleander distillate (0.2- 8 mg/ml). At least 10 embryos were used for each con- centration. Aqueous distillate of Nerium oleander was obtained by hydrodistillation method. Dose-dependent effects of Nerium ole- ander on embryonic developmental parameters such as total morphological score, yolk sac diameter, crown-rump length and somite number were compared using morphological method. Embryos were also evaluated for the presence of any malformations. Results: Compared with the controls, the addition of different concentrations of Nerium oleander did not cause any growth retardation and/ or malformations. Conclusions: These results indicate that the use of Nerium oleander in pregnancy may not affect the developing embryos negatively. However, these findings should be supported by further in vivo experiments as well as cell-line studies.

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