Hasan Basri ULUSOY, Hülya GÜLTEKİN, Nuran KÜÇÜK

Hidrojen peroksidin dana koroner arterini gevşetici etkisinde L-tipi voltaja bağlı kalsiyum kanallarının rolünün araştırılması

Amaç: Bu in vitro çalışmada, hidrojen peroksidin L-tipi voltaja bağımlı kalsiyum kanallarını etkileyip etkilemediğinin araştırılması amaçlandı. Yöntem: Dana sol koroner arterinin inen ön dalından elde edilen halkalar rastlantısal şekilde bir kontrol ve iki deneme grubuna ayrıldı. Tüm halkalar 37 oC derecede Krebs-Henseleit solüsyonu içeren ve % 95 O2 - % 5 CO2 karışımı ile sürekli olarak gazlandırılan 10 ml hacminde organ banyosu içine alındı. 1,5 g gerim altında 90 dakika dinlenme periyodunu takiben halkalara ilaç ve maddeler uygulandı. Bulgular: Tüm gruplarda, kümülatif tarzda uygulanan hidrojen peroksit (10-7 – 10-2 M), serotonin (10-6 M) ile ön kasılma oluşturulan halkalarda doza bağlı gevşemeler oluşturdu. Deneme gruplarındaki gevşeme cevaplarının, L-tipi voltaja bağlı kalsiyum kanal blokeri nifedipin (10-6 M) ve L-tipi voltaja bağlı kalsiyum kanal açıcı BAY K-8644 (10-7 M) ile değiştirilmediği gözlendi (P>0.05). Sonuç: Bu veriler ile hidrojen peroksidin L-tipi voltaja bağımlı kalsiyum kanalları üzerine etkisinin olmadığı söylenebilir.

Evaluation of the role of L-type voltage dependent calcium channels on relaxing effect of hydrogen peroxide in bovine coronary artery

Objective: In this study, it was aimed to investigate whether hydrogen peroxide effect L-type voltage-gated calcium channels. Methods: The rings obtained from bovine left anterior descending coronary artery randomly assigned into one control (n=6) and two experimental groups (n=6). All of the rings were placed into 10 ml organ baths containing Krebs-Henseleit solution at 37 oC continuously bubbled with 95% O2 - 5% CO2 gas mixtures. Following 90 minutes resting period under 1,5 g tension, drugs and chemicals were applied to the rings. Results: In all groups, hydrogen peroxide, in a cumulative manner (10-7 – 10-2 M), induced dose-dependent relaxations in the rings precontracted with serotonin (10-6 M). It was observed that the relaxation responses in experimental groups were not changed by L- type calcium channel blocker, nifedipine or L-type voltage dependent calcium channel opener, BAY K-8644 (10-7 M). Conclusion: According to these data it may suggest that hydrogen peroxide has no effect on L-type voltage dependent calcium channels.

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