Enteral beta glukanın ratlarda oluşturulan deneysel kısa barsak modelinde intestinal adaptasyon üzerindeki etkisi

Amaç: Bu çalışmanın amacı ratlarda masif ince barsak rezeksiyonu ve Anostomoz yapılarak oluşturulan deneysel kısa barsak sendromu modelinde enteral beta glukanın intestinal adaptasyona olan etkilerini araştırmaktır. Yöntem: Çalışmada 30 adet dişi Wistar rat kullanıldı. Ratlar 3 eşit gruba ayrıldı (sham, kontrol ve tedavi). Kontrol ve tedavi grubunda % 80 ince barsak rezeksiyonu yapılmıştır. Tedavi grubunda beta glukan 25 mg/gün orogastrik gavaj yöntemiyle enteral uygulandı. İki hafta sonunda ratlar sakrifiye edildi. Makroskobik ve mikoroskobik değişimleri değerlendirildi. Bulgular: Çalışma sonucunda tedavi grubunda barsak uzunluklarının anlamlı düzeyde arttığı ve villüs yüksekliği, mukozal kalınlık, kript derinliği gibi mikroskobik bulgularda da artma olduğu gözlenmiştir. Sonuç: Enteral beta glukanın masif ince barsak rezeksiyonu sonrasında kalan barsakları belirgin olarak uzattığı, mikroskobik adaptasyon parametrelerini arttırdığı görüldü. Bu etkinlikte lenfoproliferatif özelliğinin rol oynayabileceği sonucuna varıldı.

Effects of enteral beta glucan in a rat model of short bowel on intestinal adaptation

Objective: The aim of this study was to search the effects of enteral beta glucan to intestinal adaptation at rats which have been performed massive intestinal resection and anastomosis. Methods: Thirty female Wistar rats were included in this study. Rats were divided in to 3 groups (sham, control, and treatment). In control and treatment group 80% small bowell resection was performed. In treatment group, 25 mg/day beta glucan was given by orogastric feeding route. Rats were sacrificed on 14th day. Macroscopic and microscopic changes were recorded. Results: In the treatment group intestinal length, mucosal thickness, villus height and crypt depth were increased statistically significantly. Conclusion: In this study we observed the effects of beta glucan on intestinal length and microscopically changes after massive intestinal resection, as well as the effects of beta glucan on intestinal lymphoproliferation.

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