MEHMET SALİH KAYA, MEHMET KARA, HANEFİ ÖZBEK

Çörek otu (Nigella sativa) tohumunun insan hücresel bağışıklık sisteminin CD3+, CD4+, CD8+ hücreleri ve toplam lökosit sayısı üzerine etkileri

Amaç: Çörek otu (Nigella sativä) tohumunun insanlarda hücresel bağışıklık sisteminin T lenfosit alt grupları ve toplam lökosit sayısı üzerine etkisinin araştırılması. Yöntem: Çalışma, yaş ortalaması 24 olan 30 gönüllü erkek üzerinde yapıldı. Uygulama öncesinde her bir gönüllüden EDTA'lı standart kan sayım tüplerine 2 ml venöz kan örneği alınarak Coulter Epics XL Flow Sitometri cihazında CDS+ (olgun T lenfosit), CD4+ (yardımcı T lenfosit) ve CD8+ (süpresör-sitotoksik T lenfosit) hücreleri, Coulter STKS cihazında ise toplam lökosit sayımı yapıldı ve sonuçlar kontrol olarak kullanıldı. Deneklere 4 hafta süresince her gün 30 mg/kg çörek otu tohumu oral yoldan verildi ve 4. hafta sonunda tekrar kan örnekleri alınarak analiz edildi. Bulgular: CD3+ ve total lökosit değerlerinde istatistiksel açıdan anlamlı bir artış olduğu (sırasıyla P

Anahtar Kelimeler:

Nigella sativa, Bağışıklık sistemi, Bağışıklık, hücresel, Lökositler, Lenfosit alt grupları, CD8-pozitif T-lenfositler, CD4-pozitif T-lenfositler

The effects of black cumin (Nigella sativa) seeds on CD3+, CD4+, CD8+ cells of human immune system and total number of leucocytes

Objective: To investigate the effect of black cumin (Nigella sativa) seeds on T lymphocyte subsets of human immune system and total leukocytes. Methods: This study was conducted on 30 volunteered males with a mean age of 24. At the beginning, 2 ml of venous blood samples were taken from the volunteers and kept in standard glass test tubes with EDTA. CDS+, CD4+ and CD8+ cells in samples were counted with Coulter Epics XL flow cytometry equipment and total leukocytes with Coulter STKS; this data was used as control. The volunteers were asked to have 30 mg/kg of black cumin seeds every day for 4 weeks. At the end of fourth week, the blood samples were also taken from the volunteers and were analyzed. Results: It was observed that CDS+ and total leukocytes were increased significantly (P<0.01 and P<0.001, respectively). But the changes in other parameters were increased insignificant. Conclusion: In conclusion, it may be assumed that black cumin seeds may increase activity of human immune system.

Keywords:

Nigella sativa, Immune System, Immunity, Cellular, Leukocytes, Lymphocyte Subsets, CD8-Positive T-Lymphocytes, CD4-Positive T-Lymphocytes,

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