Akut yüzme egzersizi yaptırılan diyabetik sıçanlarda intraperitoneal çinko sülfat uygulamasının kas dokusundaki lipid peroksidasyonuna etkisi

Amaç: Bu çalışmanın amacı, streptozotosin ile diyabet oluşturulmuş akut yüzme egzersizi yaptırılan sıçanlarda çinko uygulamasının kas dokusundaki lipid peroksidasyonu ve antioksidan kapasite üzerindeki etkisinin araştırılmasıdır. Gereç ve Yöntem: Spraque-Dawley cinsi 80 adet erişkin erkek ratlar kullanılan çalışmada. deney hayvanları eşit sayıda 8 gruba ayrıldı: Grup 1, genel kontrol. Grup 2, çinko uygulanan kontrol. Grup 3, çinko uygulanan diyabetli kontrol. Grup 4, yüzme kontrol. Grup 5, çinko uygulanan yüzme. Grup 6, çinko uygulanan diyabetli yüzme. Grup 7, diyabetli yüzme. Grup 8, diyabet grubu. Diyabet oluşturmak için hayvanlara 40 mg/kg dozunda intraperitoneal (ip) streptozotosin (STZ) enjekte edildi. Enjeksiyonlar 24 saat sonra aynı dozda tekrarlandı. Son enjeksiyonlardan 6 gün sonra kan glukozu 300 mg/dl ve üzerinde olan hayvanlar diyabetik olarak kabul edildi. Hayvanlara 4 hafta boyunca 6 mg/kg/gün ip çinko sülfat verildi. Dört hafta süren çalışmanın bitiminde deney hayvanlarından dekapitasyonla alınan kas doku örneklerinde MDA (nmol/gram/ protein) ve GSH (mg/dl/gram protein) düzeyleri tayin edildi. Bulgular: Kas dokusundaki en yüksek MDA değerleri grup 4 ve 7’de elde edildi. Grup 5 ve 6’nın kas MDA seviyeleri grup 4 ve 7’den düşük, diğer grupların tamamından yüksekti. Grup 5 ve 6 en yüksek kas GSH değerlerine sahipti. Grup 4’ün aynı parametresi grup 5 ve 6’dan düşük diğer grupların tamamından daha yüksekti. Kas dokusundaki en düşük GSH düzeyleri Grup 7 ve 8’de elde edildi. Sonuç: Çalışmanın sonuçları diyabetik sıçanlarda zorlu yüzme egzersizinin yol açtığı kas dokusundaki lipit peroksidasyonu üzerinde intraperitoneal çinko sülfat uygulamasının koruyucu rolü olduğunu göstermektedir.

Objective: The objective of the present study is to explore the effect of zinc supplementation on lipid peroxidation and antioxidant capacity in the muscle tissue of rats which were induced diabetes with streptozotocin and subjected to acute swimming exercise. Material and Method: The study included 80 adult, male, Spraque-Dawley type rats, which were equally allocated to 8 groups. Group 1, general control. Group 2, zinc-supplemented control. Group 3, zinc-supplemented diabetic control. Group 4, swimming control. Group 5, zinc-supplemented swimming. Group 6, zinc-supplemented diabetic swimming. Group 7, diabetic swimming. Group 8, diabetes group. The animals were injected with 40 mg/kg intraperitoneal (ip) streptozotocin (STZ) to induce diabetes. The injections were repeated after 24 hours. The animals whose blood glucose stood at or over 300 mg/dl on day 6 following the last injection were considered diabetic. The animals were supplemented with 6 mg/kg/day ip zinc sulfate for 4 weeks. At the end of the 4-week study, the animals were decapitated to collect muscle tissue samples, which were analyzed in terms of MDA (nmol/gram/protein) and GSH (mg/dl/gram protein) levels. Results: The highest MDA values in the muscle tissue were established in groups 4 and 7. Muscle MDA levels in groups 5 and 6 were lower than those in groups 4 and 7, but higher than the levels in all other groups. Groups 5 and 6 had the highest muscle GSH values. Group 4 had this parameter lower than groups 5 and 6, but higher than others. The lowest GSH levels in the muscle tissue were obtained in groups 7 and 8. Conclusion: Results of the study demonstrate that zinc sulfate supplementation has a protective role against lipid peroxidation caused by strenuous swimming exercise in the muscle tissue of diabetic rats.

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