Multiple Shoot and Callus Formation in Different Explants of The Medicinally Important Peganum harmala L.

In this study, the micropropagation potential of the medicinally important Peganum harmala L. plant containing valuable metabolites has been examined. For this purpose, hypocotyl and shoot tips isolated from P. harmala plants were applied different concentrations and combinations of plant growth regulators of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indole acetic acid (IAA). Best callus growth were obtained in hypocotyl explants in Murashige and Skoog (MS) medium that contained 1 mg L-1 NAA and 1 mg L -1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contained 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to the soil with a success rate of 72%.

Tıbbi Öneme Sahip Peganum harmala L. Bitkisinin Farklı Eksplantlarında Çoklu Sürgün ve Kallus Oluşumu

Bu çalışmada içerdiği metabolitler nedeni ile tıbbi öneme sahip olan Peganum harmala L. bitkisinin mikroçoğaltım potansiyeli çalışılmıştır. Bu amaçla, P. harmala bitkilerinden izole edilen hipokotil ve sürgün ucu eksplantlarına farklı konsantrasyonlarda ve kombinasyonlarda benzil amino pürin (BAP), naftalen asetik asit (NAA) ve indol asetik asit (IAA) bitki büyüme düzenleyicileri uygulanmıştır. Yapılan uygulamalar sonucunda, en iyi kallus gelişimi hipokotil eksplantlarında 1 mg L-1 NAA ve 1 mg L-1 BAP içeren Murashige ve Skoog (MS) besin ortamında elde edilmiştir. Eksplant başına düşen sürgün sayısı (5.42±0.32 adet) ve sürgün uzunluğu (2.95±0.06 cm) en iyi 1.5 mg L-1 BAP içeren MS besin ortamında belirlenmiştir. Elde edilen sürgünlerin köklendirilmesi amacıyla IBA uygulamaları yapılmıştır. Çalışmalar sonucunda elde edilen köklenmiş bitkiler %72 başarı ile toprağa aktarılmışladır.

Kaynakça

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