Bazı biber hat ve çeşitlerinin kontrollü şartlar altında Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 ırk1’e karşı reaksiyonu
Birçok sebzede verim ve kaliteyi olumsuz yönde etkileyen Kök-ur nematodları biberde de önemli zararlar oluşturmaktadır. Bu zararlılarla mücadelede kullanılan kimyasalların, çevreye ve insan sağlığına yapmış olduğu olumsuz etkiler ,mücadelede alternatif kontrol yaklaşımlarından biri olan dayanıklı çeşitlerin kullanımı seçeneğini önemli kılmaktadır. Dayanıklı çeşit kullanımının ekonomik ve çevreye duyarlı olması, nematodlarla mücadelede bu yöntemi cazip hale getirmektedir. Bu çalışmada, Poliakrilamid jel elektroforez (PAGE) ve morfolojik karakterlerden biri olan anal kesit yöntemi ile tür teşhisi yapılan Meloidogyne incognita ırk1’e karşı, baharat yapımına uygun bazı kırmızıbiber hat ve çeşitlerinin reaksiyonları incelenmiştir. Kök-ur nematodlarının ırk belirlemesi, Kuzey Karolina Konukçu Testi ile yapılmıştır. Reaksiyon çalışması, tesadüf parselleri deneme desenine göre 4 tekerrürlü olacak şekilde yürütülmüştür. Denemede toplam 16 hat ve 2 biber çeşidi kullanılmıştır. Yumurta kümesi-reaksiyon skalasına (0-5) göre reaksiyonuna bakılan hat ve çeşitler M. incognita ırk1’e karşı hassas bulunmuştur. Kontrol olarak kullanılan dayanıklı CM334 bitkisi 0 (nematodsuz) skala değerini almıştır.
Reactions of Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 race 1 against some pepper lines and varieties in controlled conditions
Affecting yield and quality of many vegetable varieties across to the world, root knot nematodes can also cause severe damages on many pepper varieties. The adverse effects of environment and human health triggered by the chemical use make researchers to find an alternative to this management treatment for nematode control. Use of resistant varieties drove enormous attention as one of important management strategies recently. Use of resistant varieties is a preferable cheap and environmentally friendly method that has a potential to be an alternative to the chemical control methods. In this study, the reactions of some spice production appropriate red pepper varieties and lines against Meloidogyne incognita race 1 was determined. Root knot nematode species confirmation was made by Polyacrylamide Gel Electrophoresis (PAGE), and perineal pattern morphology. Race determination of nematode was made by North Carolina host differential test. The experiment was designed as a randomized complete block design with 4 replications. Total of 16 lines and 2 varieties were used in the study. All lines and varieties were evaluated by egg mass-reaction scale (0-5) index. The study revealed that all lines and varieties of pepper were sensitive to M. incognita race 1.The resistant control variety, CM334, presented 0 egg mass (no nematode) indices in the study.
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