Remazol Ultra Kırmızı RGB reaktif boyar maddesinin metabolik olarak aktif kefir biyokütlesi ile biyosorpsiyonu

Bu çalışmada, canlı formda kefir biyokütleleri ile sulu çözeltilerden Remazol ultra Kırmızı RGB boyar maddesinin biyosorpsiyon yöntemi ile giderimi incelenmiştir. Biyosorpsiyon prosesini optimize etmek ve modellemek amacı ile deneyler kesikli olarak, farklı pH (2 - 2,5 - 3 - 3,5 - 4 - 5 - 6 - 7 - 8), sıcaklık (18- 25 - 32 - 39°C), çalkalama hızı (0 - 140 - 300 rpm) ve başlangıç boyar madde konsantrasyonlarında (50 - 75 - 100 - 200 - 400 mg/L) gerçekleştirilmiştir. pH 2’de, 25°C sıcaklıkta, 100 mg/L başlangıç boyar madde konsantrasyonunda, 2,4 g/L kefir biyokütlesi kullanılarak gerçekleştirilen 180 dakikalık biyosorpsiyon işlemi sonunda % 93 oranında boyar madde giderimi elde edilmiştir. Kefir biyokütlesi için maksimum adsorpsiyon kapasitesi değeri 112 mg/g olarak 400 mg/L başlangıç boyar madde konsantrasyonunda elde edilmiştir. Ardışık olarak gerçekleştirilen biyosorpsiyon deneyleri kefir biyokütlelerinin tekrar kullanılabilir olduğunu göstermiştir. Gerçekleştirilen modelleme çalışmaları sonucunda, kefir biyokütleleri ile boyar madde biyosorpsiyon kinetiğinin yalancı birinci derece kinetik modeli ile, biyosorpsiyon dengesinin ise Temkin izoterm modeli ile ifade edilebileceği belirlenmiştir.

Anahtar Kelimeler:

Remazol Kırmızı RGB, biyosorpsiyon, kefir biyokütlesi, kinetik, izoterm

Biosorption of reactive dye Remazol Ultra Red RGB by metabolically active kefir biomass

In the present study biosorptive removal of Remazol Ultra Red RGB textile dye from aqueous solutions with living kefir biomass was investigated. The experiments were carried out at batch operation mode under different process conditions such as initial pH (2 - 2.5 - 3 - 3.5 - 4 -5- 6 - 7 - 8), temperature (18 - 25 - 32 - 39°C), shaking rate (0 - 140 - 300 rpm) and initial dye concentration (50 - 75 - 100 - 200 - 400 mg/L) to optimize and model the biosorption process. 93% dye removal was attained in 180 min processing time by using 2.4 g/ kefir biomass for 100 mg/L initial dye concentration at pH 2 and 25°C. The maximum adsorption capacity for kefir biomass was obtained as 112 mg/g at 400 mg/L dye concentration. Successively performed biosorption experiments showed that the kefir biomass is reusable. As a result of the modelling studies performed the biosorption kinetics and equilibrium by kefir biomass were best described respectively with pseudo-first-order and Temkin models.

Keywords:

Remazol Red RGB, biosorption, kefir biomass, kinetic, isotherm,

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