AHMET BOZDAĞ, YAVUZ SELİM İLHAN, İbrahim ÖZERCAN, Süleyman AYDIN

Ratlarda Parsiyel Hepatik Rezeksiyon Sonrası Ghrelin'in Karaciğer Rejenerasyonu Üzerine Etkisi

ÖZET Amaç: Karaciğer, organizmanın en büyük organı olup, metabolik fonksiyonların düzenlenmesinde büyük rol oynar. Karaciğer rejenerasyonunun kontrol edilebilir yaygın bir işlem haline gelmesi modern cerrahideki en önemli aşamalardan birisidir. Ratlarda growth hormon uyarımı, karaciğerde kitlesel büyüme, hücre sayısında ve ekstraselüler doku miktarında artış sonucunda büyümeye ve vücut ağırlığında artışa neden olur. Ghrelin büyüme hormonunun salgılanması, enerji metabolizması ve iştah regülasyonu üzerine etkilidir. Bizim bu çalışmadaki amacımız parsiyel hepatektomi yapılan ratlarda Ghrelinin karaciğer rejenerasyonu üzerine olan etkilerini araştırmaktır. Gereç ve Yöntem: Bu çalışmada 30 adet Wistar Albino türü rat kullanıldı. Ratlara genel anestezi altında parsiyel hepatektomi yapılıp, grup Ideki ratlara intraperitoneal serum fizyolojik, grup IIdeki ratlara intraperitoneal ghrelin uygulandı. Gruplar 24 saat, 48 saat, 7 gün yaşatılan ve beşer rattan oluşan alt gruplara ayrıldı. Belirtilen süre sonunda ratlar eter anestezisi sonrası dekapite edilip kan örnekleri alındı ve serum AST, ALT, ALP, GGT, ghrelin, growth hormon, PTZ düzeyleri ölçüldü. Sonra relaparatomi uygulanıp total hepatektomi yapıldı ve alınan karaciğerden doku ghrelin düzeyi ve Ki-67 ekspresyon oranı değerlendirildi. Bulgular: Grup IIdeki elde edilen morfolojik ve biyokimyasal parametreler grup I ile karşılaştırıldığında rölatif karaciğer ağırlığı, AST, ALT, ALP, GGT, albumin, PTZ, serum açile ve doku desaçile ghrelin düzeylerinde anlamlı farklılık gözlendi (p0,05). Sonuç: Çalışmamızda karaciğer rejenerasyonunu gösteren indirekt bulgularda pozitif sonuç elde edilmişken direkt bulgularda anlamlı bir sonuç bulunamamıştır. Bu ghrelinin antiproliferatif etkisinden kaynaklanmış olabileceği düşünülmüştür. Ghrelinin karaciğer rejenerasyonuna katkısı çalışmamızda tespit edilmemiştir.

The Effect of Ghrelin on Hepatic Regeneration After Partial Hepatectomy in Rats

The Effect of Ghrelin on Hepatic Regeneration After Partial Hepatectomy in Rats Objective: Liver is the largest solid organ in the body having major roles in metabolic functions. Control of the hepatic regenaration is an important step in modern surgery. Growth hormone has a positive effect on cellular and extracellular growth and volume of the liver. Ghrelin has effects on hormone release and energy metabolism and appetite regulation. The aim of this study is to evaluate the effects of ghrelin on hepatic regenaration. Material and Method: Thirty Wistar Albino rats were used in this study. Partial hepatectomy was performed to rats, and serum saline was given intraperitoneally to Group I (n=15) rats, ghrelin to group II (n=15) rats. Group II was divided to subgroups containing 5 rats in each group according to postoperative time, 24 hours, 48 hours and 7 days respectively. Under general anesthesia rats were decapitised and serum samples were taken to detect AST, ALT, ALP, GGT, ghrelin, growth hormone and PTT. After relaparotomi livers were examined for ghrelin levels and Ki-67 expressions. Results: When Group II was compared with Group I the morphological and biochemical parameters, relative liver weight, AST, ALT, ALP, GGT, albumin, PTT, acile and deacile tissue ghrelin levels were significantly different in Group II (p<0,05). But the Ki-67 and growth hormone levels were not signifacantly high in Group II (p>0,05) Conclusion: We concluded that the indirect markers of hepatic regeneration with ghrelin were positively correlated but the direct signs of the regeneration were not significantly related. This effect was atributed to the antiproliferative effect of ghrelin. In this study we concluded that ghrelin was not effective on hepatic regenaration.

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