An electrophoretic taxonomic study on serum proteins of Acanthobrama marmid, Leuciscus cephalus, and Chondrostoma regium

Bu calismada, Karakaya Baraj Golu (Malatya)'nden yakalanan Leuciscus cephalus, Acanthobrama marmid and Chondrostoma regium ( Cyprinidae)'nin serum proteinleri dogal poliakrilamid gel elektroforez (Native-PAGE) ve Sodyum dodeosil sulfat-poliakrilamid jel elektroforezine (SDS-PAGE) uygulandi. Elektroforegramlar uc turun serum proteinlerinin molekuler agirliklari arasinda benzerlik ve arkliliklarin varligini gostermistir. L. cephalus, A. marmid and C. regium'un total serum protein bandlari Native-PAGE'de sirasiyla 5, 8, 7 ' dir. SDS-PAGE'de L. cephalus icin 13, A. marmid icin 11 ve C. regium icin 11 protein bandi elde edildi. Native- PAGE'den elde edilen elektroforegramlarda protein bandlarinin molekuler agirliklari 80.3 kD protein bandi haric, uc balik turunde farkli bulundu. Uc balik turunde protein bandlarinin molekuler agirliklari SDS-PAGE'de 21.7 kD olarak belirlendi. 63.4, 52.3 and 49.5 kD protein bandlari sadece L. cephalus ve C. regium'da mevcuttu. Diger taraftan, diger protein bandlarinin molekuler agirliklarina yakin oldugu goruldu.

Acanthobrama marmid, Leuciscus cephalus ve Chondrostoma regium'un serum proteinleri üzerine taksonomik bir çalışma

In this study, native polyacrylamide gel electrophoresis (Native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were applied to the serum proteins of Leuciscus cephalus, Acanthobrama marmid and Chondrostoma regium (Cyprinidae) fish taken from) the Karakaya Dam (Malatya, Turkey). The electrophoregrams showed that there were similarities and differences in the molecular weight (MW) of the serum proteins among the three species. In the Native-PAGE, the total number of serum protein bands of L. cephalus, A. marmid and C. regium were 5, 8, 7, respectively. In the SDS-PAGE, 13 protein bands were obtained in L. cephalus, 11 in A. marmid and 11 in C. regium. In the electrophoregrams obtained from native-PAGE, the MW of the protein bands were found to be different in the fish species except the 80.3 kD protein band which was detected in the three fish species. In the SDS-PAGE, the protein band of 21.7 kD was observed in the three fish species. Protein bands of 63.4, 52.3 and 49.5 kD were present only in L. cephalus and C. regium. On the other hand, it was seen that other protein bands had similar molecular weights.

Kaynakça

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