Türkiye’de Mersin İlinde Köpeklerde Canine Parvovirus 2’nin Moleküler Karakterizasyonu ve Filogenetik Analizi

Canine parvovirus 2 (CPV-2) genç köpeklerde akut kanlı gastroenteritis ile seyreden oldukça bulaşıcı ve ölümcül bir viral hastalıktır. 1978’de ABD’de ilk ortaya çıktıktan sonra, virus genomunun sürekli evrimi, tüm dünyada CPV2a, CPV-2b, new CPV-2a, new CPV-2b ve CPV-2c gibi yeni genetik ve antijenik varyantların ortaya çıkmasına yol açmıştır. Bu çalışmanın amacı, Mersin (Türkiye) ilinde klinik olarak hasta köpeklerin kan örneklerinde CPV -2’yi tespit etmek ve dolaşımda olan CPV- 2 genetik varyatlarının karakterizasyonunu sağlamaktır. Bunun için 2017 Nisan ve 2017 Eylül ayları arasında, yerel kliniklerden 20 adet kan örneği gastroenteritisli köpeklerden toplandı. 20 örneğin 7’si PZR ile CPV- 2 yönünden pozitif olarak test edildi. Klinik CPV-2b örneklerinin VP2’sindeki amino asit karşılaştırmaları sonucunda daha önce çalışılan Türk soylarından farklı olarak Ile-324 mutasyonu tespit edildi. VP2 geni dizinlerinden köken alarak yapılan filogenetik ağaç, Mersin ilinden gelen CPV-2b dizilerinin Hindistan genetik varyantlarıyla ilişkili olduğunu göstermiştir. Aynı zamanda Mersin ilinden elde edilen CPV-2b dizinleri daha önce tespit edilen Türk genetik varyantlarından farklı bir dal şekillendirmiştir. Bu sonuçlar Mersin ilinde CPV-2b genetik varyantının köpekler arasında dolaştığını göstermiştir.

Molecular Characterization and Phylogenetic Analysis of Canine Parvovirus 2 in Dogs, Mersin Province, Turkey

Canine parvovirus type 2 (CPV-2) is a highly contagious and lethal viral disease with acute gastroenteritis in young dogs. After it first appeared in USA in 1978, continuous evolution of virus genome has arisen new genetic and antigenic variants such as CPV-2a, CPV-2b, new CPV-2a, new CPV-2b and CPV-2c throughout the world. The aim of the present study was to detect CPV-2 genetic variants from blood samples of clinically ill dogs and to characterize CPV-2 genetic variants circulating in Mersin province (Turkey), by partial sequence analysis of VP2 gene. A total of 20 blood samples were collected from dogs with gastroenteritis from local veterinary clinics between April 2017 to September 2017. Out of 20, 7 samples were positive for CPV-2 genomic DNA using conventional PCR. Amino acid comparisons showed that Ile-324 mutation was detected in the VP2 of clinical CPV-2b samples, as differ previously studies Turkish genetic variants. A phylogenetic tree constructed based on VP2 gene sequences indicated that CPV-2b sequences from Mersin province were related to Indian genetic variants. Moreover, CPV-2b sequences from Mersin have clustered separately from other Turkish CPV-2b genetic variants previously reported. The results show that CPV-2b genetic variant is circulating among in dogs in Mersin province.

Kaynakça

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Kaynak Göster

APA Dinçer, E . (2017). Molecular Characterization and Phylogenetic Analysis of Canine Parvovirus 2 in Dogs, Mersin Province, Turkey . Etlik Veteriner Mikrobiyoloji Dergisi , 28 (2) , 96-100 . DOI: 10.35864/evmd.523432