Güzin İPLİKÇİOĞLU ÇİL, Fatma Seda BİLİR ORMANCI

Piliç Boyun Derilerinden İzole Edilen C. perfringens’lerin Antibiyotik Direnç Profillerinin Belirlenmesi

Mikroorganizmaların antibiyotiklere karşı geliştirdikleri direnç, halen tüm dünyada önemini koruyan bir halk sağlığı sorunudur. Antibiyotik direnç ile mücadelede patojen mikroorganizmaların, gıdalarda güvenilir metotlarla tespiti, identifikasyonu ve antibiyotik direnç profillerinin belirlenmesi büyük önem taşımaktadır. Bu çalışmanın amacı, üç farklı kanatlı kesimhanesinden alınan 180 piliç boyun derisi örneğinden izole edilen 35 Clostridium perfringens suşunun antibiyotik direnç profillerinin belirlenmesidir. İzolatların antibiyotik direnç profillerinin tespitinde CLSI (Clinical and Laboratory Standards Institute) tarafından bildirilen disk difüzyon metodu kullanılmıştır. İzolatların 35’inin de (%100) en az bir antibiyotiğe dirençli olduğu, ayrıca 31 (% 88,5) C. perfringens izolatının 1’den fazla antibiyotiğe direnç gösterdiği tespit edilmiştir. İzolatların tamamının (% 100) dirençli olduğu antibiyotik trimetoprim olarak belirlenmiş, bu antibiyotik yanında, % 65,7 ile tetrasiklin, % 62,8 oranında gentamisin direncin yüksek oranda tespit edildiği diğer antibiyotikler olmuştur. Elde edilen veriler izolatların hepsinin vankomisine duyarlı olduğunu da ortaya koymuştur.

Anahtar Kelimeler:

Antibiyotik direnç, C. perfringens, disk difüzyon, piliç eti

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