Cevat NİSBET, Gül Fatma YARIM, Gülay ÇİFTCİ, Nilgün GÜLTİKEN, Sena ÇENESİZ

Laktasyon Periyodundaki Subklinik Mastitisli Koyunlarda Kkan ve Süt Laktoferrin Konsantrasyonlarının Araştırılması

Bu çalışmada, laktasyon periyodundaki subklinik mastitisli ve sağlıklı Karayaka ırkı koyunlarda süt ve kan serumlarında laktoferrin konsantrasyonlarının belirlenmesi amaçlandı. Araştırmada, ortalama 2-3 yaşlarında ve laktasyonlarının orta döneminde bulunan Karayaka ırkı koyunlar, Kaliforniya mastitis test ve sütte somatik hücre sayımı yöntemleri ile subklinik mastitis yönünden incelendi. Sütleri Kaliforniya mastitis test pozitif ve somatik hücre sayımı 300.000-1.000.000 olan 23 adet koyun subklinik mastitis grubunu, Kaliforniya mastitis test negatif ve somatik hücre sayımı 0.05). Süt serumu laktoferrin konsantrasyonları subklinik mastitisli ve kontrol gruplarında sırasıyla 23.98±3.45 μg/ml ve 16.84±1.29 μg/ml olarak bulundu. Subklinik mastitisli grubun süt serumu laktoferrin düzeylerinin kontrol grubununkine göre istatistiksel olarak yüksek olduğu belirlendi (p

Anahtar Kelimeler:

Etlik Veteriner Mikrobiyoloji Dergisi, Koyun, Laktasyon, Laktoferrin, Subklinik Mastitis, Süt, Cevat NİSBET, Gül Fatma YARIM, Gülay ÇİFTÇİ, Nilgün GÜLTEKİN, Sena ÇENESİZ, Sena ÇENESİZ, 2013

Investigation of Blood and Milk Lactoferrin Concentrations in Lactating Ewes With Subclinical Mastitis

The aim of the present study was to determine the availability of blood and milk lactoferrin concentrations in the diagnosis of subclinical mastitis in lactating Karayaka ewes. The milk samples obtained from 43 mid-lactating Karayaka ewes, aged between 2 and 3, were examined by California Mastitis Test (CMT) and somatic cell count (SCC) methods. Animals (n=23) had positive CMT score and SCC between 300.000-1.000.000 cells/ml were defined as subclinical mastitis group, whereas ewes with CMT negative and SCC between 100.000-200.000 cells/ml were described as control (healthy) group. The serum were obtained from blood and milk samples of ewes and ELISA was performed to detect blood and milk lactoferrin concentrations. The mean blood lactoferrin concentrations in subclinical mastitis group and control group were 0.90±0.08 μg/ml and 0.85±0.08 μg/ml, respectively, however the difference was not significant (p>0.05). The mean milk lactoferrin concentrations in ewes with subclinical mastitis and healthy ewes was 23.98±3.45 μg/ml and 16.84±1.29 μg/ml, respectively (p

PDF

___

Adlerova L, Bartoskova B , Faldyna M, (2008). Lactoferrin: a review.Veterinarni Medicina. 53, 457-468.
Alacam E, (1989). Diseases of cattle, Veterinary Medicine Teknografik Matbaa, İstanbul, Turkey. 459-482.
Al-Majali A, Ismail ZB, Al-Hami Y, Nour Ay, (2007). Lactoferrin concentration in milk from camels (camelus dromedarius) with and without subclinical mastitis. Int J Appl Res Vet Med. 5, 120-124.
Avci G, Sel T, (2004). Milk and serum lactoferrin levels in Angora Goats during lactation period. Ankara Üniv Vet Fak Derg, 51, 181-187.
Baker EN, (2005). Lactoferrin: a multi-tasking protein par excellence. Cell Mol Life Sci. 62 (22), 2529-2530.
Baveye S, Elas E, Mazurier J, Spik G, Legrand D, (1999). Lactoferrin: a multifunctional glycoprotein involved in the modulation of the inflammatory process. Clin. Chem. Lab. Med. 37, 281-286.
Bergonier D, Berthelot X, (2003). New advances in epizootiology and control of ewe mastitis. Livest Prod Sci. 79, 1-16.
Brock JH, (2002). The physiology of lactoferrin. Biochem Cell Biol. 80, 1-6.
Buchta R, (1991) Ovine lactoferrin: isolation from colostrum and characterization. J Dairy Res. 58(2):211-218.
Chen Gh, Yin Li, Chiang JH, Jiang ST, (2008). Cloning and expression of antibacterial goat lactoferricin from Escherichia coli AD494(DE3)pLysS expression system. J Food Prot. 71 (12), 2523-2525.
Chen PW, Shyu C, Mao FC, (2003). Antibacterial activity of short hydrophobic and basic-rich peptides. Am J Vet Res. 64 (9), 1088-1092.
Cheng JB, Wang JQ, Bu DP, Liu GL, Zhang CG, Wei HY, Zhou LY, Wan JZ, (2008). Factors Affecting the Lactoferrin Concentration in Bovine Milk. J Dairy Sci. 91, 3, 970-976.
Contreras A, Sierra D, Sanchez A, Corrale JC, Marco JC, Paape MJ, Gonzalo C, (2007). Mastitis in small ruminants. Small Rum Res. 68, 145-153.
De Jong G, Van Dijk JP, Van Eijk HG, (1990). The biology of transferrin. Clin Chim Acta. 190, 1-46.
Graaf T, Dwinge RH, (1995). Estimation of milk production losses due to subclinical mastitis in dairy cattle in Costa Rica. In proceeding of the third IDF international mastitis seminar, 11, 28 May-1 June, Tel-Aviv, Israel.
Hagiwara SI, Kawa K, Anri A, Nagahata H, (2003). Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J Vet Med Sci. 65 (3), 319-323.
Kanyshkova TG, Buneva VN, Nevinsky GA, (2001). Lactoferrin and its biological functions. Biochemistry (Moscow). 66, 1-7.
Kawai K, Hagiwara S, Anri A, Nagahata H, (1999). Lactoferrin concentration in milk of ovine clinical mastitis. Vet Res Commun. 23, 391-398.
Kilicoglu Ç, Alacam E, Izgur H, Akay O, Wiesner HU, (1989). Eutergesundheitskontrolle von Milchkülen im Gebiet von Ankara (Turkei). Dtsch Tierärztl Wschr. 96, 486-488.
Lafi SQ, (2006). Use of somatic cell counts and California Mastitis Test results from udder halves milk samples to detect subclinical intramammary infection in Awassi sheep. Small Rum Res. 62, 83-86.
Legrand D, Elass E, Carpentier M, Mazurier J, (2005). Lactoferrin: a modulator of immune and inflammatory responses. Cell Mol Life Sci. 62, 2549-2559.
Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J, (2008). Lactoferrin structure and functions. Adv Exp Med Biol. 606, 163-94.
Leitner G, Chaffer M, Shamay A, Shapiro F, Merinu E, Ezra A, Saran A, Silanikove N, (2004). Changes in milk composition as affected by subclinical mastitis in sheep. J Dairy Sci. 87, 46-52.
Levay PF, Viljoen M, (1995). Lactoferrin: a general review. Haematologica. 80, 252-267.
Mazurier J, Lhoste JM, Montreuil J, Spik G, (1983). Comparative study of the iron-binding properties of human transferrins. II. Electron paramagnetic resonance of mixed metal complexes of human lactotransferrin. Biochim Biophys Acta. 30, 745 (1), 44-49.
Moguilevsky N, Retegui La, Masson PL, (1985). Comparison of human lactoferrins from milk and neutrophilic leucocytes. Relative molecular mass, isoelectric point, iron-binding properties and uptake by the liver. Biochem J. 229 (2), 353-359.
Oberg G, Lindmar G, Moberg L, Venge P, (1983). The peroxidase activity and cellular content of granule proteins in PMN during pregnancy. Br J Haematol. 55 (4), 701-708.
Rao CR, (1973). Linear Statistical Inference and Its Applications, John&Sons, New York
Schalm OW, Carroll Ej, Jain NC, (1971). Bovine Mastitis, Lea and Febiger, Philadelphia. P, 24.
Silanikove N, Shapiro F, Leitner G, Merin U, (2005). Subclinical mastitis affects the plasmin system, milk composition and curd yield in sheep and goats: comparative aspects. In: Hogeveen, H. (Ed.), Mastitis in Dairy Production. Wageningen Academic Press Publishers, The Netherlands, 511-516.
Sordillo LM, Streicher KL, (2002). Mammary Gland Immunity and Mastitis Susceptibility. Journal of Mammary Gland Biology and Neoplasia, 7, 2, 135-146.
Teng CT, Beard C, Gladwell W, (2002). Differential expression and estrogen response of lactoferrin gene in the female reproductive tract of mouse, rat and hamster. Biol Reprod. 67, 1439-1449.
Tripathi BN, (2000). Diseases of the mammary glands of goats and sheep. Vet Bul. 70, 1117-1142.
Valenti P, Antonini G, (2005). Lactoferrin: an important host defense against microbial and viral attack. Cell Mol Life Sci. 62, 2576-2587.
Vorland LH, (1999). Lactoferrin: a multifunctional glycoprotein. APMIS. 107, 971-981.
Weinberg ED, (2003). The therapeutic potential of lactoferrin. Expert Opin Investig Drugs. 12 (5), 841-51.
Welty FK, Smith KL, Schanbacher FL, (1976). Lactoferrin concentration during involution of the bovine mammary gland. J Dairy Sci. 59, 224-231.