Ayşenur ÇETİN, Yavuz ULUSOY, Ayşe ÖĞÜTÇÜ, Fatma Gökçe UZUN, Filiz DEMİR

Dichlorvos’un Ratların İnce Bağırsak Dokusu Üzerine Etkisi ve Vitamin C ve E’nin Koruyucu Rolü

Organofosforlu bir pestisit olan dichlorvos zirai zararlıların kontrolünde yaygın olarak kullanılmaktadır. Bu çalışmada vitamin C (200mg/kg)+vitamin E (200mg/kg), dichlorvos (1,6mg/kg) ve vitamin C (200mg/kg)+vitamin E (200mg/kg)+dichlorvos erkek ratlara oral olarak 7 hafta süre ile uygulandı. Muameleden 24 saat, 4 hafta ve 7 hafta sonra ratların ince bağırsak dokularındaki histopatolojik değişiklikler ışık mikroskobu kullanılarak, kontrol grubu ile mukayeseli olarak incelendi. Dichlorvos uygulandıktan 4 ve 7 hafta sonra ince bağırsakta villuslarda kısalma ve genişleme tespit edildi. Vitamin C+vitamin E+dichlorvos uygulanan ratların ince bağırsak dokularında da benzer patolojik değişiklikler gözlendi. Sonuç olarak düşük doz dichlorvos ratların ince bağırsak dokularında patolojik değişikliklerin oluşmasına yol açmaktadır. Vitamin C ve vitamin E’nin, dichlorvosun ince bağırsak dokusunda meydana getirdiği patolojik değişiklikleri önleyemediği tespit edildi.

Anahtar Kelimeler:

Etlik Veterinerlik Mikrobiyoloji Dergisi, Dichlorvos, İnce Bağırsak, Doku, Vitamin C, Vitamin E, Ayşenur ÇETİN, Yavuz ULUSOY, Ayşe ÖĞÜTÇÜ, Fatma Gökçe UZUN, Filiz DEMİR, 2008

Effects of Dichlorvos in Small Intestine Tissue of Rats and Protective Role of Vitamins C and E

Dichlorvos, an organophosphorus pesticide, is widely used in agricultural control. In the present study, vitamin C (200mg/kg)+vitamin E (200mg/kg), dichlorvos (1,6mg/kg) and vitamin C (200mg/kg)+vitamin E (200mg/kg)+dichlorvos were given to male rats orally for 7 weeks. Histopathological changes in the small intestine tissues were investigated using light microscope at the end of 24h, 4th and 7th weeks comparatively with control group. After 4 and 7 weeks of dichlorvos exposure shortening and dilatation were observed in intestinal villi. Same histopathological changes in the small intestine tissues were observed at the end of 24h, 4th and 7th weeks. As a result, lower doses of dichlorvos caused histopathological changes in the rat small intestine tissues. It’s determined that vitamin C and vitamin E haven’t prevented the pathological changes caused by dichlorvos in small intestine tissue.

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Carr RL, Richardson JR, Guarisco JA, Kachroo A, Chambers JE, Couch TA, Durunna GC, Meek EC, (2002). Effect of PBC exposure on the toxic impact of organophosphorus insecticides. Toxicol Sci 67, 311-321.
Choudhary S, Gill KD, (2001). Protective effect of nimodipine on dichlorvos-induced delayed neurotoxicity in rat brain. Biochem Pharmacol. 62, 1265–1272
Desi I, Nagymajtenyi L, (1999). Electrophysiological biomarkers of an organophosphorus pesticide, dichlorvos. Toxicol Lett 1, 55-64.
Kalender S, Kalender Y, Ateş A, Yel M, Olcay E, Candan S, (2002). Protective role of antioxidant vitamin E and catechin on idarubicin-induced cardiotoxicity in rats. Braz J Med Biol Res. 35, 1379-1387.
Kalender S, Kalender Y, Ogutcu A, Uzunhisarcikli M, Durak D, Acikgoz F, (2004). Endosulfan-induced cardiotoxicity and free radical metabolism in rats: The protective effect of vitamin E. Toxicology 3, 227-235.
Kalender S, Ogutcu A, Uzunhisarcikli M, Acikgoz F, Durak D, Ulusoy Y, Kalender Y, (2005a). Diazinoninduced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology 211, 197-206.
Kalender, Y., Yel, M., Kalender, S., (2005b). Doxorubicin hepatotoxicity and hepatic free radical metabolism in rats. The effects of vitamin E and catechin. Toxicology. 209 (1), 39-45.
Kalender Y, Uzunhisarcıklı M, Ogutcu A, Acikgöz F, Kalender S, (2006). Effects of diazinon pseudocholinesterase activity and haematological indicates in rats:The protective role of vitamin E. Environ Toxicol Phar. 22,46– 51.
Kalender S, Kalender Y, Durak D, Ogutcu A, Uzunhisarcikli M, Cevrimli BS, Yildirim M, (2007). Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E. Pestic Biochem Phys. 88, 213– 218.
Maitra SK, Mitra A, (2008). Testicular functions and serum titers of LH and testosterone in methyl parathion-fed roseringed parakeets. Ecotoxicol Environ Safe. 71, 236- 244.
Ogutcu A, Uzunhisarcikli M, Kalender S, Durak D, Bayrakdar F, Kalender Y, (2006). The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E. Pestic Biochem Phys. 86, 93-98.
Ogutcu A, Suludere Z, Kalender Y, (2008). Dichlorvosinduced hepatotoxicity in rats and the protective effects of vitamins C and E. Environ Toxicol Pharm. 26, 355–361.
Okamura A, Kamijima M, Shibata E, Ohtani K, Takagi K, Ueyama J, Watanabe Y, Omura M, Wang H, Ichihara G, Kondo T, Nakajima T, (2005). A coprehensive evaluation of the testicular toxicity of dichlorvos in Wistar rats. Toxicology 213, 129-137.
Öğütcü A, Ulusoy Y, Kahraman K, Uzunhisarcıklı M, Uzun FG, Taştan H, (2007). Metil parathion’un sıçanların ince bağırsak dokusu üzerine etkisi ve vitamin C ve E’nin koruyucu rolü. Etlik Vet Mikrobiyol Derg 18, 21-26.
Raheja G, Gill KD, (2002). Calcium homeostasis and dichlorvos induced neurotoxicity in rat brain. Mol Cell Biochem. 232, 13-18 (2002).
Sarin S, Gill KD, (1999). Dichlorvos induced alterations in glucose homeostasis: possible implications on the state of neuronal function in rats. Mol Cell Biochem. 199, 87-82.
Senthil Kumar J, Banudevi S, Sharmila M, Murugesan P, Srinivasan N, Balasubramanian K, Aruldhas MM, Arunakaran J, (2004). Effects of Vitamin C and Vitamin E on PCB (Aroclor 1254) induced oxidative stress, androgen binding protein and lactate in rat Sertoli cells. Reprod Toxicol. 19, 201-208.
Ulusoy Y, Toprak B, Uzunhisarcıklı M, Öğütcü A, (2004). Diazinonun sıçan hepatositleri üzerine etkisinin electron mikroskobu ile incelenmesi. Etlik Vet Mikrobiyol Derg 15, 1-2.
Ural MŞ, Köprücü SŞ, (2006). Acute toxicity of dichlorvos on Fingerling European Catfish, Silurus glanis. Bull Environ Contam Toxicol 76, 871-876.
Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Buyukkomurcu F, (2007). Acute, subacute and subchronic administration of methyl parathioninduced testicular damage in male rats and protective role of vitamins C and E. Pestic Biochem Phys. 87, 115– 122.
Yarsan E, Cakir O, (2006). Effects of dichlorvos on lipit peroxidation in mice on subacute and subchronic periods. Pestic Biochem Physiol 86, 106-109.
Young, IS, Woodside JV, (2001). Antioxidants in health and disease. J Clin Pathol. 54,76–86.