Bir inekte Ovine Herpesvirüs-2’nin neden olduğu Sığır Gangrenli Nezlesi olgusu

Sığır gangrenli nezlesi (SGN) (malign kataral ateş), evcil sığır ve yabani geviş getirenlerin önemli viral bir hastalığıdır. Sunulan olguda, 4 yaşlı Holştayn ırkı bir inekte Ovine Herpesvirüs-2’nin (OvHV-2) neden olduğu SGN enfeksiyonu patolojik ve PCR bulgularıyla birlikte tanımlanmıştır. Mikroskopik incelemede, tüm doku ve organlarda fibrinoid nekrotik vaskülitis, beynin damar duvarlarında yangısal değişiklikler (pons, serebrum ve meninksler), oral mukozada ülseratif değişiklikler, böbreğin korteksinde glomerüllerin çevresinde interstisyumda hafif yangısal hücre infiltrasyonları ve kalpteki kas demetleri arasında yaygın ödem görüldü. OvHV-2 nükleik asitlerinin varlığını belirlemek amacıyla PCR yöntemi uygulandı ve 386 bp ürünü, OvHV-2'nin Pol gen bölgesini kopyalayan primer sekansları ile elde edildi. Bu bulgular ile sunulan olguda koyunla ilişkili SGN tanısı doğrulandı.

Anahtar Kelimeler:

Sığır gangrenli nezlesi, Ovine herpesvirüs-2, Patoloji, PCR

Malignant Catarrhal Fever caused by Ovine Herpesvirus-2 in a cow

Malignant catarrhal fever (MCF) has been defined as a viral disease of domestic cattle and wild ruminants. We report a case of MCF caused by ovine herpesvirus-2 (OvHV-2) in a 4-year-old female Holstein cow. Microscopic examination showed that fibrinoid necrotic vasculitis in all tissues and organs; inflammatory changes in the vessel walls of the brain (pons, cerebrum and meninges); ulcerative changes in the oral mucosa; mild inflammatory cell infiltrations around the glomeruli and interstitium in the cortex of the kidney; widespread edema between the muscle bundles in the heart. PCR was performed to determine the presence of ovine herpesvirus-2 nucleic acids and 386 bp product was obtained by primer sequences that replicated the Pol gene region of OvHV-2. This PCR finding confirmed our diagnosis as sheep-associated MCF (SA-MCF).

Keywords:

Malignant catarrhal fever, Ovine herpesvirus-2, Pathology, PCR,

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