Akut Tolüen Maruziyetinin Tavşan Beyin Korteksinde Yol Açtığı Toksik Etkiler
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Bu çalışmada yüksek dozda tolüenin tavşan beyin korteksi üzerine akut dönemdeki histopatolojik ve biyokimyasal etkilerinin araştırılması amaçlandı. On yedi adet Yeni Zelanda cinsi tavşan (4-6 kg) iki guruba ayrıldı. Grup I kontrol grubu (n=7) olarak kullanılırken Grup II’ye (n=10) yüksek doz Tolüen verildi (876 mg/kg/ip). Üç saatlik deney periyodunun sonunda öldürülen hayvanlardan biyokimyasal analizler için kan serumu ve beyin doku örnekleri % 10’luk nötral formaline alınıp parafine gömülerek kesildi (5 μm). H&E (hematoksilen-eozin), Crystal violet boyamaları, Na, K, GSH, MDA ve NO kan serum seviyeleri analiz edildi. Bu çalışma sonucunda Tolüen verilen tavşanlarda önemli ölçüde histopatolojik bozukluklar ve ciddi amiloit plak oluşumu gözlemlenmiştir. Tolüen grubunda Na, K, MDA, kan serumu seviyelerinde önemli ölçüde artış görülmüşken, GSH ve NO seviyelerinde anlamlı derecede azalma izlenmiştir. Bu çalışma sonucunda yüksek doz tolüenin beyin korteksinde akut dönemde ciddi hasarlar oluşturabileceği gösterilmiştir.
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- 1. Agency for Toxic Substances and Disease Registry
(ATSDR), (2000). Toxicological Profile for Tolüene. http://
www.atsdr.cdc.gov/toxprofiles/tp56.pdf. Erişim tarihi:
25.10.2016.
2. Altındag A, Ozkan M, Oto R, (2001). İnhalanla ilişkili bozukluklar.
Klinik Psikofarmokoloji Bülteni. 11:143-148.
3. Arends YM, Duyckaerts C, Rozemuller JM, Eikelenboom
P, Hauw JJ, (2000). Microglia, amyloid and dementia in
alzheimer disease. A correlative study. Neurobiol Aging.
21:39-47.
4.Argo A, Bongiorno D, Bonifacio A, Pernice V, Liotta R,
Indelicato S, Zerbo S, Fleres P, Ceraulo L, Procaccianti P,
(2010). A fatal case of a paint thinner ingestion: comparison
between toxicological and histological findings. Am J
Forensic Med Pathol. Jun;31(2), 186-91.
5. ATSDR (Agency for Toxic Substances and Disease Registry),
(2006). Case Studies in Environmental Medicine. Tolüene
Toxicity. http://www.atsdr.cdc.gov/csem/Tolüene/.
Erişim tarihi: 25.10.2016.
6. Balster RL, (1998). Neural basis of inhalant abuse. Drug
Alcohol Depend. 51,207-14.
7. Baydas G, Reiter RJ, ve ark., (2003). Melatonin protects the
central nervous system of rats against Tolüene-containing
thinner intoxication by reducing reactive gliosis. Toxicol
Lett. 137, 169-174.
8. Bowen SE, Batis JC, Mohammadi MH, Hannigan JH,
(2005). Abuse pattern of gestational Tolüene exposure and
early postnatal development in rats. Neurotoxicol Teratol.
27, 105-116.
9. Calderon-Guzman D, Espitia-Vazquez I, Lopez-Dominguez
A, Hernandez-Garcia E, Huerta-Gertrudis B, Coballase-
Urritia E, ve ark., (2005). Effect of Tolüene and nutritional
status on serotonin, lipid peroxidation levels and NA+/K+-
ATPase in adult rat brain. Neurochem Res. 30, 619-624.
10. Champe PC, Harvey RA, (1997). Glikozaminoglikanlar.
Tokullugil A, Dirican M, Ulukaya E,. Lippincott’s illustrated
reviews serisinden: Biyokimya. İkinci baskı, Nobel
Tıp Kitabevi, İstanbul. p. 147-156.
11. Canoruç N, Çiçek R, Atamer A, Dursun M, Turgut C,
Güneli E, Canoruç F, (2001). Protective effects of vitamin E
selenium and allopurinol against stres-induced ulcer formation
in rats. Turk J med Sci. 31,199-203.
12. Chavan S, Sava L, Saxena V, Pillai S, Sontakke A, Ingole
D, (2005). Reduced glutathione: Importance of specimen
collection. Indian Journal of Clinical Biochemistry. 20(1),
150-152.
13. Dickson DW, (1997). The pathogenesis of senile plaques. J
Neuropath Exp. Neurol. 56, 321-339.
14. Dossing, M, Aelum, JB, Hansen, SH, Lundqvist, GR,
Andersen, NT, (1983). Urinary hippuric acid and orthocresol
excretion in man during experimental exposure to
Tolüene. Br J Ind Med. 40, 470-473.
15. Dündaröz MR, Turkbay T, Akay C, Sarıcı SU, Aydın A,
Denli M, Gökçay E, (2003). Antioxidant enzymes and lipid
peroxidation in adolescent with inhalant abuse. Turk J
Pediatr. 45(1),43-45.
16. Faust RA, (1994). Toxicity Summary for Tolüene. OAK
Ridge Reservation Enviromental Restoration Program.
Chemical Hazard Evaluation Group Biomedical and
Environmental Information Analysis Section Healt
Sciences Research Division. http://riskassessment.ornl.
gov/documents/Tolüene.pdf. Erişim tarihi: 25.10.2016.
17. Georganopoulou DG, Chang L, Nam JM, ve ark., (2005).
Nanoparticle-based detection in cerebral spinal fluid of a
soluble pathogenic biomarker for Alzheimer’s disease. Proc
Natl Acad Sci USA. 102, 2273.
18. Gong Y, Chang L, Viola KL, ve ark., (2003). Alzheimer’s
Disease affected brain: presence of oligomeric A beta ligands
(ADDLs) suggests a molecular basis for reversible
memoory loss. Proc Natl Acad Sci USA. 100, 10417.
19. Guzelian P, Mills S, Fallon HJ, (1988). Liver structure and
function in print workers exposed to Tolüene. J Occup Med.
30, 791-796.
20. Ilgazlı A, Şengül C, Maral H, Özden M, Erçin C, (2004).
The effects of thinner inhalation on superoxide dismutase
activities, malondialdehyde and glutathione levels in rat
lungs. Clin Chim Acta. 343(1-2),141-4.
21. Jones HE, (1997). Neurobehavioral consequences of intermittent
prenatal exposure to high consentrations of Tolüene.
Neurotoxicology and Teratology. 4, 305-13.
22. Kanter M, (2011). Protective effects of thymoquinone on
the neuronal injury in frontal cortex after chronic Tolüene
exposure. J Mol Histol. 42(1), 39-46.
23. Karagözler AA, Mehmet N, Batçıoğlu K, (2002). Effects of
long-term exposure on blood cytokine levels and antioxidant
enzyme activities in house painters. J Toxicol Environ
Health A. 65(17),1237-46.
24. Knowles RB, Wyart C, Buldyrev SV, Cruz L, Urbanc B,
Hasselmo ME, Stanley HE, Hyman BT, (1999). Plaqueinduced
neurite abnormalities: implications for disruption
of neural networks in Alzheimer’s disease. Proc Natl Acad
Sci U S A. 96,5274-5279.
25. Kuyumcu A, Duzgun AP, Ozmen MM, Besler HT,
(2004). Travma ve enfeksiyonda nitrik oksidin rolü. Ulus
Travma Dergisi. 10(3),149-159.
26. Matthew BG, Jourd’heuil D, Wink DA, (1999). Nitric oxide
I. Physiological chemistry of nitric oxide and its metabolites:
implications in inflammation. Am. J. Physiol.
276,315-321.
27. Mohan IK, Dsa UN, (1997). Oxidant stress, antioxidants
and essential fatty acids in bronchial asthma. Med. Sci.
Res. 25,307-309.
28. Murray RK, Granner DK, Mayes RA, Rodwell VW, (1996).
Fizyolojik öneme sahip lipidler. Dikmen N, Özgünen
T, Harper’ın Biyokimyası, Yirmidördüncü baskı, Barış
Kitabevi, İstanbul.
29. Naslund J, Haroutunian V, Mohs R, ve ark., (2000).
Correlation between elevated levels of amyloid beta-peptide
in the brain and cognitive decline. JAMA. 283, 1571.
30. OEHHA (Office of Environmental Health Hazard
Assessment), (1999). Public Health Goal for Tolüene in
Drinking Water. http://oehha.ca.gov/water/phg/pdf/
tolu_f.pdf. Erişim tarihi: 20.10.2016.
31. Palmer RMJ, Ferrige AG, Moncada S, (1993). Nitric oxide
release accounts fort he biological activity of endotheliumderived
relaxing factor. Nature. 327,524-6.
32. Park CK, Kwon KT, Lee DS, Jo CM, Tak WY, Kweon YO,
ve ark., (2003). A case of toxic hepatitis induced by habitual
glue sniffing. Korean J Hepatol. 4, 332-336.
33. Park E, Park Y, Gwak Y, (1998). Oxidative damage in
tissues of rats exposed to cigarette smoke. Free Radical
Biology & Medicine. 25(1),79–86.
34. Park JJ, Chang H, Jung JY, Jung SJ, Song KH, Suh WN,
ve ark., (2006). A Case of Tolüene-induced Renal Tubular
Acidosis Presented with Hypokalemic Paralysis. www.jksem.
org. 17(6), 656-658.
35. Tomei F, Giuntoli P, Biagi M, Baccolo TP, Tomao E, Rosati
MV, (1999). Liver damage among shoe repairers. Am J Ind
Med. 36, 541-547.
36. Thomas JA, (1999). Including glutathione, a peptide for
cellular defense against oxidative stress. Free Radical
Biology and Medicine. 27,916-921.
37. Tsatsakis AM, Dolapsakis G, Troulakis G, Christodoulou
P, Relakis K, Trikilis N, ve ark., (1997). Fatal and nonfatal
outcome by accidental intoxication with paint thinner.
J Clin Forensic Med. 4, 133-137.
38. Ulakoğlu EZ, Saygı A, Gümüştüş MK, Zor E, Öztek İ,
Kökoğlu E, (1998). Alterations in superoxide dismutase,
lipid peroxidation and glutathione levels in thinner inhaled
rat lungs: Relationship between histopathological properties.
Pharmacological Research. 38(3),209-214.