Tülin GUVEN GOKMEN, Elçin GÜNAYDIN, Osman SEZER, Pınar AYVAZOĞLU DEMİR, Armağan Erdem ÜTÜK

Adana İlinde Sığırlarda Anaplasma marginale ve Anaplasma centrale’nin Serolojik ve Moleküler Yöntemler ile Araştırılması

Anaplazmozis tropikal ve subtropikal iklim bölgelerinde vektörler tarafından taşınan yaygın bir hastalıktır. Özellikle büyükbaş hayvancılık işletmelerinde son yıllarda sık tespit edilmeye başlanmıştır. Bu çalışmanın amacı, Adana ilindeki sığırlarda Anaplasma spp. prevalansını belirlemekti. Bu amaçla Adana'nın farklı iklim bölgelerine sahip on beş ilçesinde sığırlardan 187 kan örneği alınarak kompetetif ELISA (cELISA) ve Nested-PCR yöntemleriyle incelenmiştir. Sığırlarda seropozitiflik%38,5 (72/187) olarak belirlenmiştir. Ayrıca moleküler prevalans, Nested-PCR yöntemleriyle Anaplasma centrale için%1,6 (3/187), Anaplasma marjinale için%3,2 (6/187) olarak tespit edilmiştir. Bu çalışma ile Türkiye'nin Adana ilinde sığır anaplazmozisi ile ilgili epidemiyolojik verileri ayrıntılı bir şekilde ele alınmış ve elde edilen verilerin hastalığın önlenmesine ve kontrol programlarına katkıda bulunacağı düşünülmüştür.

Anahtar Kelimeler:

A.centrale, A.marginale, cELISA, Nested-PCR, Adana

Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods

Anaplasmosis is a common disease in tropical and subtropical climate zone and is transmitted by vectors. Especially in large cattle management systems, it has started to be detected frequently in recent years. The aim of this study was to determine the prevalence of Anaplasma spp. in cattle in Adana province. For this aim, 187 blood samples were collected from cattle from fifteen districts of Adana that have different climatic zones and examined by Competitive ELISA (cELISA) and Nested-PCR methods. Seropositivity was determined as 38.5% (72/187) in cattle. The molecular prevalence was detected as 1.6% (3/187) for Anaplasma centrale and 3.2% (6/187) for Anaplasma marginale by Nested-Polymerase Chain Reaction (PCR) methods. In this study, epidemiological data related to bovine anaplasmosis in Adana province of Turkey were discussed in detail and it was thought that the obtained data would contribute to disease prevention and control programs.

Keywords:

A.centrale, A.marginale, cELISA, Nested-PCR, Adana,

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