Arı Poleni Bazlı Gümüş Nanopartiküllerin Sentezi, Karakterizasyonu ve Antidiyabetik Potansiyeli

Arı poleni içerdiği yağ, karhohidrat, lipit ve fenolik bileşenler sayesinde hem önemli bir besin kaynağı olmakta hem de apiterapi uygulamalarında yer almaktadır. Yapılan bu çalışma ile arı poleninin içerdiği bileşenlerin potansiyel indirgeme güçlerinden faydalanılarak çevre dostu, biyouyumlu arı poleni temelli gümüş nanopartiküller sentezlendi (BP- AgNPs). Elde edilen nanopartiküller spektroskopik ve mikroskobik teknikler kullanılarak karakterize edildi. Gümüş nanopartiküllerin 348 nm’ de maksimum absorbans verdiği tespit edildi. Partikül boyutlanırınn ortalama 40-60 nm arasında değiştiği görüldü. Elde edilen BP-AgNPs’ in α-amilaz ve α-glukozidaz enzimleri üzerine inhibisyon etkisi araştırıldı. IC50 değerleri α-amilaz ve α-glukozidaz enzimleri için sırasıyla 2.56 ± 0.10ve 2.13 ± 0.11 mg/mL olarak tespit edildi. Elde edilen BP- AgNPs’in Diabetes mellitus tedavisinde destekleyici olarak kullanılabileceği belirlendi.

Anahtar Kelimeler:

Yeşil sentez, enzim inhibisyonu, nanopartikül, Diabetes mellitus, çevre dostu

Synthesis, Characterization and Antidiabetic Potential of Bee Pollen Based Silver Nanoparticles

Bee pollen is both an important food source and a part of apitherapy applications because of the components it contains such as fat, carbohydrate, lipid and phenolics. In this study, environmentally friendly, biocompatible bee pollen-based silver nanoparticles (BP-AgNPs) were synthesized by utilizing the potential reducing powers of the components contained in bee pollen. The resulting nanoparticles were characterized using spectroscopic and microscopic techniques. It was determined that silver nanoparticles gave maximum absorbance at 348 nm. It was observed that the particle sizes ranged between 40-60 nm on average. The inhibition effect of the obtained BP-AgNPs on α-amylase and α-glucosidase enzymes was investigated. IC50 values were determined as 2.56 ± 0.10 and 2.13 ± 0.11 mg/mL for α-amylase and α-glucosidase enzymes, respectively. It was determined that obtained BP-AgNPs could be used as a support in the treatment of Diabetes mellitus.

Keywords:

Green synthesis, enzyme inhibition, nanoparticles, Diabetes mellitus, eco-friendly,

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