CANDAN ÇİÇEK, Ayşe Kübra KARABOĞA ARSLAN, EYLEM ULAŞ SAZ, HÜSNÜ PULLUKÇU, GÜRSEL ÇOK

Üst ve alt solunum yolu enfeksiyonu olan hastalarda solunum virüslerinin multipleks PCR yöntemi ile eşzamanlı saptanması

Amaç: Bu çalışmada, üst ve alt solunum yolu enfeksiyonu olan hastalarda multipleks PCR tanı yöntemi ile kısa sürede solunum virüslerinin saptanması amaçlan dı . Gereç ve Yöntem : Bu çalışmay a, Ocak 2007-Ağustos 2013 tarihleri arasında, üst veya alt solunum yolu enfeksiyonu ön tanısı alan, toplam 1708 (1220 çocuk , 488 erişkin ) hasta (%45. 1 kadı n, %54. 9 erkek, yaş aralığı 5 gün -94 yaş ) dahil edildi. Hastaların solunum yolu örneklerindeki solunum virüsleri , mültipleks PCR yöntemi ile araştırıldı . Bulgular: Toplam 1708 hastanın, 620sinde (%36.3) bir veya birden fazla solunum virüsü pozitif, 1088 inde (%63.7) negatif bulundu. Bu hastaların 63ünde (%3.7) çoklu etken saptandı. Çoklu etken saptanan örneklerin %89.5i çocuk hastalardı. Solunum yolu örneklerinin 387si (%22.7) poliklinik hastalarından , 1321i (%77.3) yatan hastalardan elde edildi. Poli klinik hastalarının %48.5i, yatan hastaların %32.7sinde (p

Simultaneous detection of respiratory viruses using multiplex PCR method in patients with upper and lower respiratory tract infection

Aim: In this study, we aimed to determine the respiratory viruses in a short time by multiplex PCR method in patients with upper and lower respiratory tract infections. Materials and Methods: In this report, between January 2007 and August 2013, a total of 1708 patients (1220 children, 488 adult; 45,1% female, 54.9% male , age range: 5 days-94 years) prediagnosed as having upper or lower respiratory tract infections were enrolled in the study. Respiratory viruses were investigated by multiplex PCR method in respiratory samples of these patients.Results: In a total of 1708 patients, 620 (36.3%) of them were positive for one or more respiratory viruses and 1088 (63.7%) were negative. In 63 of the patients (3.7%) multiple viruses were detected. Most of the samples (89.5%) containing multiple agents were detected in children. Respiratory specimens were obtained from 387 (22. 7%) outpatients and 1321 (77.3%) inpatients. One or more respiratory viruses were detected in 39.2% of pediatric patients, 29.1% of adult patients (p<0. 000), 48.5% of outpatients and 32.7% of inpatients. In all samples, influenza A(H1N1)pdm09 virus, RSV, rhinovirus and influenza A virus were found be positive in 12.1%, 9.0%, 3.7% and 3.0%, respectively. Conclusion: Respiratory viruses were positive 40% in patients with acute respiratory tract infections. Multiple viral infections were detected in approximately 4% patients. Almost 90% of these patients were found to be of the pediatric patients. If influenza A(H1N1)pdm09 virus is kept separate, the three most common pathogen were RSV, rhinovirus and influenza A virus, respectively. Respiratory viruses were de tected more frequently in children compared to adult patients and in outpatient compared to hospitalized patients.

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