Anacak Günay YETİK, A. EROL, G.M. ÇINAR, D.J. CATRAVAS

Sıçan torasik aortları vasküler reaktive cevapları bozulmadan soğukta saklanabilir

Vasküler dokuların uzun süre canlılığının bozulmadan saklanabilmesi, hem organ transplantasyonunun hem de farmakolojik araştırmaların verimliliği açısından önemlidir. Böylece ilaçların uzun süreli etkilerini değerlendirebilmek, örnek sayısını artırmak ve insan damarlarında çalışma olanağını artırmak mümkün olabilmektedir. Bu nedenle çalışmamızda sıçan aortlarında, endotel ve vasküler düz kas fonksiyonlarının +4 °C de, Krebs-bikarbonat çözeltisinde saklamak sureti ile korunup korunamayacağını araştırmayı hedefledik. Bu amaçla sıçan torasik aortik halkaları izolasyondan hemen sonra ya da +4°C’de, Krebs-bikarbonat solüsyonunda 1 veya 15 saat bekletildikten sonra vasküler reaktivite cevapları değerlendirildi. Endotele bağımlı gevşeme cevapları 10-6 M fenilefrin ile önkastırma sonrasında asetilkolin (10-9-10-5 M), düz kasa bağımlı gevşeme cevapları ise tromboksan agonisti U46619 (3.10-8 M) ile prekontraksiyon sonrasında nitrogliserin (10-9-10-4 M) verilerek alındı. Taze izole edilmiş, +4°C’de 1 ya da 15 saat saklanmış damarların fenilefrin ve U46619‘a karşı verdikleri maksimum kasılma cevapları, endotele veya düz kasa bağımlı maksimum gevşeme cevapları ve duyarlılıkları arasında herhangi bir fark görülmedi. Bu sonuçlar, kullanılan damar türüne göre uygun solüsyon ve uygun süre seçilerek damarların vasküler fonksiyonlarının bozulmadan saklanabileceği ideal şartların sağlanabildiğini ve sıçan aortunun endotele ve düz kasa bağımlı kasılma ve gevşeme cevaplarının korunabileceği ideal şartların 15 saate kadar +4 °C’de Krebsbikarbonat solüsyonunda saklamak sureti ile elde edilebildiğini ortaya koymaktadır

Anahtar Kelimeler:

Kas kasılması, Kas gevşemesi, Koruyucular, farmasötik, Kas, düz, Gevşeme, Sıçanlar, Aort, Endotel, Soğuk ısı

Rat thoracic aorta can be cold-preserved without any change in vascular reactivity

It is important to preserve vascular functions of isolated blood vessel for productivity of both organ transplantation and pharmacological and physiological research. Thus it is possible to investigate long-term effect of drugs, increase sample number taken from the same animal and study on human blood vessel. The aim of this study was, therefore, to determine whether vascular smooth muscle and endothelial cell function could be preserved in thoracic aorta after storage in Krebs-bicarbonate solution at 4°C. Vascular reactivity has been evaluated in rat thoracic aortic rings; either fresh isolated or preserved in Krebs-bicarbonate solution at 4° C for 1 or 15 hour. Endothelium-dependent relaxation responses were evaluated by acetylcholine (10-9-10-5 M), after precontraction with phenylephrine (10-6 M) and endothelium-independent relaxations were obtained by nitroglycerine (10-9-10-4 M) following U46619-induced precontraction. There were no statistically significant differences in contractile and relaxant responses between all groups.

Keywords:

Muscle Contraction, Muscle Relaxation, Preservatives, Pharmaceutical, Muscle, Smooth, Relaxation, Rats, Aorta, Endothelium, Cold Temperature,

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