İn vitro glukoz kataraktı oluşturulmuş tavşan lenslerindeki glutatyon ve lipid peroksidasyon düzeylerine taurin'in etkisi

Bu çalışmamızda, taurinin antikataraktojenik etkiye sahip olup olmadığını in vitro glukoz kataraktı oluşturulmuş tavşan lenslerindeki redükte glutatyon (GSH) ve lipid peroksidasyon (LPO) düzeylerine göre saptamaya çalıştık. Bu amaçla, lens örnekleri korteks ve nükleus olarak iki kısıma ayrıldı; daha sonra kontrol, katarakt ve taurin çalışma grupları başlıkları altında GSH ve LPO düzeyleri belirlendi. Bulgularımıza göre, katarakt ve taurin gruplarına ait nükleus örneklerindeki GSH düzeyleri kontrollere kıyasla anlamlı bir şekilde değişmemiştir (sıra ile p=0.413 ve p= 0.792); ancak korteks örneklerinde katarakt grubunun GSH düzeyleri kontrollere kıyasla anlamlı bir şekilde azalmıştır (p=0.033). Korteks örneklerinin taurin grubundaki GSH düzeyleri ise kontrollere kıyasla farklı değildir (p=0.295). Nükleus örneklerinin kontrol, katarakt ve taurin gruplarına ait LPO düzeyleri arasında anlamlı bir farklılık yokken, korteks örneklerinin katarakt gruplarında kontrollere kıyasla LPO düzeylerinin anlamlı bir şekilde arttığı dikkat çekmektedir (p=0.022). Diğer yandan taurin grubunda elde edilen LPO düzeyleri kontrollere oldukça yakın sonuçlar vermiştir. Taurin grubunun GSH ve LPO sonuçlarına göre, lens membran lipidlerini oksidatif hasara karşı koruduğu gözlenmektedir. Sonuç olarak, taurinin antikataraktojenik bir madde olduğu söylenebilir.

Anahtar Kelimeler:

Lipid peroksidasyonu, Glutatyon, Taurin, Tavşanlar, Katarakt

The effects of taurine on the levels of glutathione and lipid peroxidation on in vitro glucose induced cataractous rabbit lenses

In this our study, we try to determine whether taurine has anticataractogenic action by the levels of glutathione (GSH) and lipid peroxidation (LPO) in vitro glucose-catarct induced rabbit lenses. With this aim, lens samples were divided into two parts as cortex and nucleus, then the GSH and LPO levels were determined under the headings of control, cataract and taurine study groups. According to our results, in the nucleus samples the GSH levels in the cataract and taurine groups showed no remarkable differences as compared to the controls (respectively p=0.413 and p=0.792), however, in the cortex samples the GSH levels in the cataract group decreased significantly relative to the controls (p=0.033). There was no considerable difference between the GSH levels in the taurine and the control groups of the cortex samples (p=0.295). Concerning the LPO levels, the control, cataract and taurine groups of the nucleus samples displayed no significant differences, whereas in the cortex samples, the cataract group was found to have significantly increased LPO levels as compared to the controls (p=0.022). On the other hand, LPO levels of the taurine group tended to yield similar results to those of the control group. According to GSH and LPO results in the taurine groups, it can be postulated that taurine may protect lens membrane lipids against oxidative destruction. In conclusion, it may be postulated that taurine is an anticataractogenic substance.

Keywords:

Lipid Peroxidation, Glutathione, Taurine, Rabbits, Cataract,

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