Homocysteine levels of rats chronically treated with nicotine

Sigara içilmesi kardiyovasküler ve serebrovasküler sistemlerde zararlı etkilere neden olur ve toksisitesinden ötürü sigaradaki nikotin tıbben önemli bir bileşiktir. Homosistein, metioninin metabolizması sırasında oluşan bir ara üründür ve homosisteinin artışı aterosklerozun patogenezinde rol oynar. Bu çalışmada 20 gün boyunca nikotin (0.35, 1.05 ve 2.1 mg/kg, subkutan) uygulanan dişi ve erkek sıçanlarda nikotinin homosistein düzeylerine etkisi araştırılmıştır. Hayvanlardan intrakardiyak olarak kan alınmış ve serum homosistein düzeyleri immünolojik yöntem ("enzyme imunoassay", EIA) ile, maruz kalınan nikotin miktarının bir göstergesi olarak kotinin düzeyleri ise immünolojik floresans polarizasyon yöntemi ("fluorescence polarization immunoassay", FPIA) He ölçülmüştür. Sıçanlara nikotin uygulaması serum kotinin düzeylerinde doza bağımlı bir artışa neden olmuştur. Kontrol ve nikotin tedavi gruplarında dişi sıçanların homosistein düzeyleri erkek sıçanlardan yüksek bulunmuştur, ancak nikotin uygulaması her iki cinste de homosistein düzeylerinde anlamlı bir değişiklik yapmamıştır. Bu bulgular, kronik olarak nikotine maruz kalış He plazma homosistein düzeyleri arasında direkt bir ilişki olmadığını göstermektedir.

Anahtar Kelimeler:

Sigara içme, Nikotin, Modeller, teorik, Homosistein, Modeller, hayvan, Sıçanlar

Kronik olarak nikotin uygulanmış sıçanlardaki homosistein düzeyleri

Cigarette smoking has detrimental effects on the cardiovascular and cerebrovascular systems and among the contents of tobacco, nicotine shows considerable medical significance because of its toxicity. Homocysteine is an intermediate metabolite of methionine and increased levels of homocysteine may underlie atherosclerosis. In the present study, we investigated the relationship between the levels of homocysteine and nicotine in female and male rats which were treated with nicotine (0.35, 1.05 and 2.1 mg/kg, subcutaneously) for 20 days. Blood was obtained by cardiac puncture and serum cotinine levels, as an index of nicotine exposed, and homocysteine levels were measured by "Enzyme lmmunoassay (EIA)" and "Fluorescence Polarization lmmunoassay (FPIA)" respectively. Nicotine administration resulted in a dose dependent increase in the serum cotinine levels of the rats. Homocysteine levels were higher in female rats than male rats both in control and nicotine-administered groups, however nicotine treatment did not significantly change homocysteine levels in either sex. These results suggest that there is no direct correlation between plasma homocysteine levels and chronic nicotine exposure.

Keywords:

Smoking, Nicotine, Models, Theoretical, Homocysteine, Models, Animal, Rats,

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