ÖZDAL F. KURT, H. S. VATANSEVER, İ. TUĞLU, S. İ. DELİLOĞLU GÜRHAN

Farklı implant materyalleri üzerinde osteoblasta farklılaştırılmış kemik iliği stromal kök hücrelerinin (KİSKH) kemik tamirindeki rolü

Amaç: Doku mühendisliği yardımı ile materyaller üzerinde kemik iliği stromal kök hücrelerinin etkileşmesinin yeni kemik oluşumuna katkı sağlayacağı düşünülmektedir. Bu çalışmada, farklı dolgu materyalleri üzerinde, medyum ile osteoblasta farklılaştırılan kemik iliği stromal kök hücrelerinin, in vivo ortamda kemik yara modeline implantasyonları sonucunda yara iyileşmesindeki etkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Erişkin erkek sıçan tibiasından elde edilen kemik iliği hücreleri, α-MEM, %10 fetal bovine serum, penisilin, streptomisin ve gentamisin içeren kontrol besi ortamında (KBO) üç farklı dolgu materyalleri olan Cellulose Tip I (BM1), Cellulose Tip II (BM2) ve Gelatine (BM3) üzerinde kültüre edildi. Yüzeye tutunmayan hücreler besi ortamının alınmasıyla uzaklaştırıldı ve materyal yüzeyine tutunmuş olan hücreler deksametazone, β-gliserofosfat ve askorbik asit içeren osteoblastik besi ortamı (OBO) ile inkübe edildi. Farklılaşan hücreler, osteonektin (ON) ve osteokalsin (OK) markırları ile osteoblasta dönme yetenekleri, vonkossa (VK) ve alkalen fosfataz (ALP) aktiviteleri ile kemikleşme ve mineralizasyon kapasiteleri saptandı. Tamirdeki etkinlikleri histolojik ve morfometrik yöntemle araştırıldı. Bulgular: Değişik materyaller üzerinde farklılaşan hücrelerin adhesyon, çoğalma ve farklılaşma kapasitelerinin arttığı ve biyomateryallerin üzerlerindeki hücreler ile implantasyonları sonrasında deneysel yara bölgesinde daha iyi tedavi yaptıkları bulundu. Sonuç: Kullanılan materyaller ile hücreler arasındaki olumlu etkileşmenin anlaşılması, gelecekte hastaların yaşam kalitesinde bir umut olacak olan doku mühendisliğinin klinik tedavideki önemi için büyük yarar sağlayacaktır.

The bone repair role of bone marrow stromal cells (BMSC) differentiated to osteoblast on the different implant materials

Introduction: It has been thought that tissue engineering could be helpful in the wound healing of the bone by the bone marrow stromal cells (BMSC) on the biomaterials. In this study, the effect of differentiated BMSC on the different biomaterials for in vivo wound healing of experimental bone defect by the implantation will be investigated. Materials and Methods: Bone marrow stromal cells from mature male rat tibia were cultured on the three different (Cellulose Type I, Cellulose type II, Gelatine) biomaterials with culture medium including α-MEM, 10% foetal bovine serum, penicillin, streptomycin, amphotericin and gentamycine. Nonadhesive cells were removed by medium change and attach cells in the culture were incubated by osteoblastic medium including ascorbic acid, dexamethasone and β-glycerophosphate. The differentiated cells were determined by osteoblastic markers such as osteonectin and osteocalcin and were analyzed by vonkossa and alkalen phosphates for their mineralization and bone formation capacity. Their healing capacity was investigated by the histologic and morphometric methods. Results: The capacity of adhesion, proliferation and differentiation of bone marrow stromal cells was positively enhanced by these biomaterials and implantation of these biomaterials with differentiated cells produced better healing on the experimental bone defect. Conclusion: Understanding of the positive interactions between used biomaterials and cells will be helpful for the importance of tissue engineering on the clinical treatment which will promise a better quality of life for patients in the future.

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