Fatma TANELİ, Cüneyt GÜNŞAR, Ahmet VAR, Cevval ULMAN, Ece ONUR, ZEKİ ARI, Bekir Sami UYANIK, Can TANELİ

Evaluation of the contralateral testis after unilateral orchidectomy by lactate dehydrogenase-C4 isoenzyme activity in rats

Torsiyon, varikosel, ve vas deferens obstruksiyonuna bağlı kontralateral testisdeki akut hasarı inceleyen bir çok araştırma yapılmıştır. Çalışmamızda, tek taraflı orşidektomi operasyonu sonrası kontralateral testiküler fonksiyonunun uzun dönem sonuçlarını incelemeyi amaçladık. Çalışmamızda 20 adet 200-250 gr ağırlığında Wistar albino erkek rat kullanılmıştır. On rafa unilateral orşidektomi uygulanmış, 10 rafa ise Sham operasyonu yapılarak control grubu olarak kullanılmıştır. 30 gün sonra ratlar sakrifiye edilmiş ve testisleri çıkarılmıştır. Testiküler fonksiyon testis dokusunda laktat dehidrogenaz izoenzimi aracılığıyla değerlendirilmiştir. Testiküler LDH-C4 izoenzimi agaroz jel elektroforez metoduyla analiz edilmiştir. LDH-C4 izoenzim aktiviteleri çalışma grubunda Sham kontroluna göre anlamlı(p

Anahtar Kelimeler:

Orşiektomi, İzoenzimler, Sıçan, Wistar, Testis, Laktat dehidrogenazlar

Ratlarda unilateral orkidektomi sonrası kontralateral testisin laktat dehidrogenaz-C4 izoenzimi ile değerlendirilmesi

There are numerous experimental studies on contralateral testicular damage induced by torsion, vahcocele and vas deference obstruction. In the present study, we aimed to investigate contralateral testicular function after unilateral orchidectomy. The study comprised 20 Wistar Albino, male rats, 200-250 g in weight. In ten rats unilateral orchidectomy was performed while the remaining were Sham operated and taken as controls. After a period of 30 days, rats were sacrificed and all testes were harvested. Testicular function was evaluated with lactate dehydragenase-C4 (LDH-C4) isoenzyme in the testicular tissue. Testicular LDH-C4 isoenzyme was assessed by agarous gel electrophoresis method. LDH-C4 isoenzyme activities were found significantly (p<0.05) decreased in the study group (7.42+6.69 vs 10.07+6.92 lU/mg wet tissue) compared with Sham controls. In conclusion, LDH-C4 isoenzyme could be a sensitive biochemical parameter showing the injury formed in the contralateral testis. The present experimental study showed that in unilateral orchidectomy the biochemical alterations were demonstrated in the contralateral testis at one month postoperatively. In a standard orchidectomy, spermatic cord ligation, vas deference obstruction, even spermatic cord torsion are inevitable; thus, during orchidectomy operations unintentional twisting of the spermatic cord, accordingly, does not seem to be appropriate.

Keywords:

Orchiectomy, Isoenzymes, Rats, Wistar, Testis, Lactate Dehydrogenases,

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