SERAP USLU, Gülperi ÖKTEM, Fati̇h OLTULU, Kenan DEMİR, Arzu İRBAN, Gulcin BASDEMİR, Ümit İNCE, AYŞEGÜL UYSAL

Evaluation of chondrogenesis and osteogenesis via Wnt/β-Catenin, S100 immunoexpression and histomorphometry in fetal rats following maternal uterine artery ligation

Aim: The aim of this study is to investigate the effects of intrauterine growth retardation depending on maternal uterine artery ligation model, Wnt/β-catenin and S100 expression immunohistochemistry and histomorphometrically on growth plate and bone tissue of fetal rats. Materials and Methods: Maternal rats were randomly divided into 3 groups (n=5). No surgery or anesthesia were applied in control group. Bilaterally the maternal uterine arteries were ligated on gestational day 18 in experimental group. Although all surgical procedures were performed in sham group, the uterine artery ligation were not made. Fetuses were taken on gestational day 20, thicknesses of growth plate and zones, trabecular number and thickness and cortical thickness were evaluated with the histomorphometrically in samples from left proximal tibia. The expressions of βcatenin and S100 immunohistochemically were evaluated in the growth plate. Results: Thicknesses of growth plate (p

Maternal uterin arter ligasyon modeli uygulanan fetal sıçanlarda kondrogenez ve osteogenezin Wnt/β-Catenin, S100 immünoekspresyon ve histomorfometri ile değerlendirilmesi

Amaç: Bu çalışmanın amacı, sıçanlarda maternal uterin arter ligasyonuna bağlı olarak gelişen intrauterin büyüme geriliği modelinde büyüme plağı ve kemik dokuda Wnt/β-catenin ve S100 ekspresyonlarının immünohistokimyasal ve histomorfometrik olarak değerlendirilmesidir. Gereç ve Yöntem: Gebe sıçanlar üç gruba ayrıldı (n=5). Kontrol grubuna cerrahi veya anestezi uygulanmadı. Deney grubunda, gebelik 18. gününde maternal uterin arterlere bilateral ligasyon yapıldı. Sham grubuna uterin arter ligasyonu dışındaki tüm cerrahi işlemler uygulandı. Fetuslar gebeliğin 20. gününde alındı, sol proksimal tibiadan büyüme plağı ve zonlarının kalınlıkları, trabeküler sayı ve kalınlıkları, kortikal kalınlıklar histomorfometrik olarak ölçüldü. Büyüme plağında β-catenin ve S100 ekspresyonları immunohistokimyasal olarak değerlendirildi. Bulgular: Deney grubunda büyüme plağı (p

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