Epileptik nöbet geçiren gebe sıçanların yenidoğanlarında karaciğer dokusunun histolojik incelenmesi
Günümüze kadar olan literatürler tarandığında, annenin gebelikte geçirdiği epileptik nöbetin yenidoğanlar üzerine olası etkilerini inceleyen çalışmaların yetersiz sayıda olduğunu görüyoruz. Sağlıklı yeni nesillere sahip olma hedeflerine katkıda bulunmak adına deneysel olarak gebe ratlarda oluşturulan epileptik model ile gebelikte geçirilen epileptik nöbetin, yenidoğan karaciğer dokularına etkilerinin incelenmesi amaçlanmıştır. Bu amaçla 225-250 gr ağırlıklı erişkin sıçanlar herbirinde altı sıçan olmak üzere üç gruba ayrıldı. Birinci gruptaki sıçanların hipokampal CA3 bölgesine gebeliğin 13. gününde stereotaksik girişimle 400 IU penisilin-G uygulandı ve sıçanların akut grand mal epileptik nöbet geçirilmeleri sağlandı. İkinci gruba intrahipokampal serum fizyolojik uygulandı (sham grup). Üçüncü grup ise kontrol olarak cerrahi işlem yapılmadan bırakıldı. Tüm gruplardaki sıçanların yavruları doğdukları gün intrakardiyak perfüzyon ile tespit edildi ve yenidoğan karaciğer dokuları ışık ve elektron mikroskopik olarak değerlendirildi. Kontrol grubunda, yenidoğan karaciğer dokusuna özgü histogenezis sürecinde normal gelişim ve maturasyon devamını gösteren bulgular saptanırken epileptik sıçanlara ait yenidoğanların karaciğer dokularının histolojisi gecikmiş maturasyon ile uyumluydu.
Histological study of liver tissue from newborn rats of mothers with epileptic seizures during pregnancy
Databases show insufficient number of studies investigating the possible effects of mother's epileptic seizures during pregnancy on the newborns. In the name of contributing to the idea of having healthy new generations this study was planned to investigate the histological alterations in the liver tissues of newborn rats from mothers experiencing epileptic seizures during pregnancy. For this purpose; adult rats (225-250 gr) were separated into three groups each having 6 rats. First group was injected with 400 III penicilin-G into the intrahippocampal CA3 region with stereotaxic device on the 13th day of pregnancy and these rats experienced acute grand mal epileptic seizure after penicilline administration. Second group was injected with intrahippocampal saline (sham group) and the third group served as the control group. Pups were sacrificed by lethal dose anesthesia on the neonatal first day and perfused with intracardiac fixative. Liver tissues was dissected, both light and electron microscopy were performed on liver slices. While, liver tissue of the newborn rats from control group showed normal hepatogenesis and hepatocellular maturation, histology of liver tissue of pups from epileptic mothers confirmed delayed maturation.
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