Fatih OLTULU, Hüseyin AKTUĞ, AYŞEGÜL UYSAL, Gülperi ÖKTEM, Nevbahar TURGAN, Nefise Ülkü YAVAŞOĞLU KARABAY, ALTUĞ YAVAŞOĞLU

Deneysel tirotoksikoz modelinde sıçan ovaryum dokusu rejenerasyon yeteneği ve kapasitesi

Amaç: Bu çalışmada, deneysel olarak tirotoksikoz oluşturulan sıçanlarda, tirotoksikoza bağlı olarak ovaryum yapısında, özellikle tiroid hormon reseptörleri içeren oosit, kumulus hücreleri ve gran ü losa hücrelerinde oluşabilecek histolojik değişimler ile doku rejenerasyon yeteneği ve kapasitesinin , kök hücre belirteçleri açısından imm ü nohistokimyasal olarak araştırılması amaçlan dı . Gereç ve Yöntem: Her biri 8 sıçandan oluşan 2 çalışma grubu oluşturul du. Tirotoksikoz grubu dişi sıçanlara 10 gün i ntraperitoneal 500µgr/kg/gün 3,3',5-Triiodo-L-thyronine enjekte edil di . On birinci gün tüm hayvanlara anestezi uygulan dı , biyokimyasal değerlendirmeler için 5 cc kan alındıktan sonra dekapite edilerek ovaryumları çıkarıl dı . Ovaryumlar immünohistokimyasal incelemeler için %4 lük paraformaldehit fiksatifi içine alın dı . Rutin immünohistokimyasal takip ve boyamalar yapıl arak tüm örnekler ış ık mikroskobunda değerlendiril di . Bulgular: İmmünohistokimyasal boyamalar sonucunda c -kit ve Thy -1 ekspresyonu kontrol grubuna göre tirotoksikoz grubunda belirgin düzeyde azalmış, Nanog ekspresyonu ise tirotoksikoz grubunda artmış olarak bulundu. Tirotoksi kozlu sıçanlarda c -kit ve Thy -1in azalması , tirotoksikozda stromal büyüme desteğinin azalmasına ve mikroçevre bozunumuna neden ol du. Sonuç: Tirotoksikozda Nanog ekspresyonunun artışı, foliküler yapıları ve oositleri korumak adına dokunun katabolik süreç (tiroid hormonlarının hemen tüm hücrelerde metabolizmayı hızlandırmasına bağlı olarak) artışına rağmen reaktif olarak mikroçevreyi koruma çabası olarak yorumlan dı .

Thyrotoxicosis in experimental rat model of ovarian tissue regeneration capability and capacity

Aim: In this study, it was aimed to investigate the possible histological changes in the ovarian structure and particularly in the oocytes, cumulus cells and granulosa cells containing thyroid hormone receptors, tissue regeneration capability and capacity in terms of stem cell markers immunohistochemically by experimentally-induced thyrotoxicosis in rats. Materials and Methods: Two study groups were formed (eight rats for each group); 500µgr/kg/day 3,3',5-Triiodo-L- thyronine was injected intraperitoneally to female rats in thyrotoxicosis group during ten days. In 11 th day, all animals were anesthesized, 5 cc blood was drawn for biochemical evaluation and then all were decapitated in order to remove ovaries. Ovaries were placed in 4% paraformaldehyde fixative for immunohistochemical investigation. Immunohystochemical processing and staining were performed routinely and all samples were evaluated by light microscopy.Results: As a result of immunohistochemical staining, c-kit and Thy-1 expressions were found to be significantly decreased in the thyrotoxicosis group compared with the control group, whereas Nanog expression was increased in the thyrotoxicosis group. Decrease in c-kit and Thy-1 expression in the thyrotoxicosis group cause d a reduction in the stimulatory effects of the growth factors on oocytes, cumulus and granulosa cells. Conclusion: Decreased levels of c-kit and Thy-1 in rat model of thyrotoxicosis lead to a decrease in stromal growth support and deterioration of microenvironment. Elevated levels of Nanog expression in thyrotoxicosis was interpreted as an effort to protect the microenvironment for the sake of follicles and oocytes despite the increase in catabolic processes (as a result of increased metabolism in almost all cells due to thyroid hormones).

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